Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability
In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefits, there have been no recent significant advances in enhancing their efficacy. Notably, Bradyrhizobiu...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/19060 https://link.springer.com/article/10.1007/s11240-024-02764-y https://doi.org/10.1007/s11240-024-02764-y |
| _version_ | 1855486292635156480 |
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| author | Serantes, Maria Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Brambilla, Silvina Maricel Serantes, Maria Laura Soto, Gabriela Cynthia Stritzler, Margarita |
| author_facet | Serantes, Maria Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_sort | Serantes, Maria Laura |
| collection | INTA Digital |
| description | In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefits, there have been no recent significant advances in enhancing their efficacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, efforts have focused on selecting spontaneous mutations in genes associated with denitrification and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system offers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants. |
| format | Artículo |
| id | INTA19060 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA190602024-08-22T10:28:42Z Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability Serantes, Maria Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel Inoculation Rhizobiaceae Bacteria Soybeans Gene Editing CRISPR Inoculación Soja Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Inoculants Inoculantes In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefits, there have been no recent significant advances in enhancing their efficacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, efforts have focused on selecting spontaneous mutations in genes associated with denitrification and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system offers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants. Instituto de Biotecnología Fil: Serantes, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Serantes, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2024-08-22T10:19:47Z 2024-08-22T10:19:47Z 2024-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19060 https://link.springer.com/article/10.1007/s11240-024-02764-y https://doi.org/10.1007/s11240-024-02764-y eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Plant Cell, Tissue and Organ Culture (PCTOC) 157 : 35 (Published online: 22 April 2024) |
| spellingShingle | Inoculation Rhizobiaceae Bacteria Soybeans Gene Editing CRISPR Inoculación Soja Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Inoculants Inoculantes Serantes, Maria Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title | Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title_full | Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title_fullStr | Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title_full_unstemmed | Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title_short | Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
| title_sort | genome editing of soybean inoculant using crispr cas9 system enhancing agricultural sustainability |
| topic | Inoculation Rhizobiaceae Bacteria Soybeans Gene Editing CRISPR Inoculación Soja Edición de Genes Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Inoculants Inoculantes |
| url | http://hdl.handle.net/20.500.12123/19060 https://link.springer.com/article/10.1007/s11240-024-02764-y https://doi.org/10.1007/s11240-024-02764-y |
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