Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymat...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/19057 https://www.mdpi.com/2071-1050/16/16/6722 https://doi.org/10.3390/su16166722 |
| _version_ | 1855486292032225280 |
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| author | Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo |
| author_browse | Bárzana, Eduardo Campos, Eleonora Gírio, Francisco Hernández-Meléndez, Oscar Marques, Susana Montiel, Carmina Ontañon, Ornella Mailen Tavares, João |
| author_facet | Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo |
| author_sort | Montiel, Carmina |
| collection | INTA Digital |
| description | The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM). |
| format | Artículo |
| id | INTA19057 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA190572024-08-22T10:05:29Z Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract from native Moesziomyces aphidis PYCC 5535T (MaPYCC 5535T), for the enzymatic hydrolysis of pretreated blue agave bagasse (BAB) at the high solids load of 20% (w/v). Three different combinations of cellulase and xylanases were evaluated. When Cellic® CTec2 was used at a dosage of 10 FPU/g oven-dried solids (ODS) supplemented with XynA or MaPYCC 5535T at an endo-xylanase dosage of 100 U/g ODS, increases in the xylose yield of 30% and 33%, respectively, were obtained. When applying in-house xylanases alone (at an endo-xylanase dosage of 100 U/g ODS), xylan in BAB was selectively hydrolyzed into xylose with 5% yield with MaPYCC 5535T, while no xylose was detected with XynA. Interestingly, a synergic effect of Cellic® CTec 2 with both xylanases was observed when using a low dosage of 1 FPU/g ODS (allowing for some liquefaction of the reaction mixture), promoting xylose and glucose release by either xylanase. A higher concentration of monomeric sugars was obtained with 10 FPU/g ODS of Cellic® Ctec 2 supplemented with 100 U/g ODS of MaPYCC 5535T, followed by XynA. The improvement in saccharification through the synergistic combination of in-house xylanases and commercial cellulases allows for the obtention of sugar-rich hydrolysates, which enhances the technical sustainability of the process. Hydrolysates were then fermented using recombinant Cellux 4TM yeast to yield 45 g/L ethanol, representing an increase of about 30% with respect to the control obtained with only the commercial cellulase cocktail. The surface modification of agave biomass with the different combinations of enzymes was evidenced by scanning electron microscopy (SEM). Instituto de Biotecnología Fil: Montiel, Carmina. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México Fil: Hernández-Meléndez, Oscar. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Ingeniería Química; México Fil: Marques, Susana. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Gírio, Francisco. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Tavares, João. Laboratório Nacional de Energia e Geologia. Unidade de Bioenergia e Biorrefinarias; Portugal Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bárzana, Eduardo. Universidad Nacional Autónoma de México. Facultad de Química. Departamento de Alimentos y Biotecnología; México 2024-08-22T09:58:05Z 2024-08-22T09:58:05Z 2024-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19057 https://www.mdpi.com/2071-1050/16/16/6722 2071-1050 https://doi.org/10.3390/su16166722 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Sustainability 16 (16) : 6722 (Agosto 2024) |
| spellingShingle | Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática Montiel, Carmina Hernández-Meléndez, Oscar Marques, Susana Gírio, Francisco Tavares, João Ontañon, Ornella Mailen Campos, Eleonora Bárzana, Eduardo Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title | Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title_full | Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title_fullStr | Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title_full_unstemmed | Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title_short | Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| title_sort | application of in house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production |
| topic | Agave Hidrólisis Enzimática Fermentación Celulasa Bioetanol Enzymatic Hydrolysis Fermentation Cellulase Bioethanol Xylanase Enzymatic Xilanasa Enzimática |
| url | http://hdl.handle.net/20.500.12123/19057 https://www.mdpi.com/2071-1050/16/16/6722 https://doi.org/10.3390/su16166722 |
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