Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis
Brucella melitensis is the etiological agent of small ruminant brucellosis and abortion is the only noticeable clinical sign in most cases. Vaccination with the attenuated Rev.1 strain is the best option to prevent this clinical manifestation and subsequently control the transmission of the disease....
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Alemán |
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Ediciones INTA
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/19047 https://doi.org/10.58149/52b1-vw79 |
| _version_ | 1855486290148982784 |
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| author | Rossetti, Carlos Alberto Rossi, Ursula Amaranta Delgado, Fernando Oscar Castaño Zubieta, Mirta Raquel Foster, Camila Nayla |
| author_browse | Castaño Zubieta, Mirta Raquel Delgado, Fernando Oscar Foster, Camila Nayla Rossetti, Carlos Alberto Rossi, Ursula Amaranta |
| author_facet | Rossetti, Carlos Alberto Rossi, Ursula Amaranta Delgado, Fernando Oscar Castaño Zubieta, Mirta Raquel Foster, Camila Nayla |
| author_sort | Rossetti, Carlos Alberto |
| collection | INTA Digital |
| description | Brucella melitensis is the etiological agent of small ruminant brucellosis and abortion is the only noticeable clinical sign in most cases. Vaccination with the attenuated Rev.1 strain is the best option to prevent this clinical manifestation and subsequently control the transmission of the disease. However, colonization of the genital tract in pregnant small ruminants is a common adverse effect observed in this and other brucellosis vaccine strains. Guinea pigs have demonstrated to be an excellent model for testing the immune-protection and efficacy of Rev.1 vaccine, but studies addressing the effects of this vaccine on pregnancy have not been fully explored. The goal of this study was to characterize the effects of subcutaneous inoculation of the B. melitensis Rev.1 on pregnant guinea pigs to evaluate the possibility of establishing a suitable laboratory animal model to test and compare the safety on pregnancy of novel vaccine candidates against small ruminant brucellosis. Mid-term pregnant guinea pigs were inoculated subcutaneously with three different concentrations of the Rev.1 strain and euthanized at late-term gestation (>50 days). Blood samples were taken for sero-response before the pregnant guinea pigs were euthanized, and samples for bacteriology and histopathology were collected during necropsy. The Rev.1 strain was more consistently isolated from the spleen, chorioallantoic placentas and fetal organs of animals inoculated with ≥107 CFU of Rev.1 than from those inoculated with a lower dose. Histological alterations varied from mild to moderate presence of inflammatory cells in the spleen, mammary gland and pregnant uterus. In conclusion, placental colonization and vertical transmission were observed in pregnant guinea pigs after being inoculated subcutaneously at mid gestation with Rev.1, which is similar to what was reported in pregnant small ruminants vaccinated against brucellosis. Therefore, the pregnant guinea pig would be a useful model to initially asses the safety of vaccine candidates in pregnancy and compare them with the currently available commercial vaccine for brucellosis in small ruminants. |
| format | Artículo |
| id | INTA19047 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Alemán |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Ediciones INTA |
| publisherStr | Ediciones INTA |
| record_format | dspace |
| spelling | INTA190472025-02-20T11:53:01Z Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis Rossetti, Carlos Alberto Rossi, Ursula Amaranta Delgado, Fernando Oscar Castaño Zubieta, Mirta Raquel Foster, Camila Nayla Cobaya Vacunación Brucelosis Control de Enfermedades Pequeños Rumiantes Vacuna Guinea Pigs Vaccination Brucella melitensis Brucellosis Diseases Control Small Ruminants Vaccines Brucella melitensis is the etiological agent of small ruminant brucellosis and abortion is the only noticeable clinical sign in most cases. Vaccination with the attenuated Rev.1 strain is the best option to prevent this clinical manifestation and subsequently control the transmission of the disease. However, colonization of the genital tract in pregnant small ruminants is a common adverse effect observed in this and other brucellosis vaccine strains. Guinea pigs have demonstrated to be an excellent model for testing the immune-protection and efficacy of Rev.1 vaccine, but studies addressing the effects of this vaccine on pregnancy have not been fully explored. The goal of this study was to characterize the effects of subcutaneous inoculation of the B. melitensis Rev.1 on pregnant guinea pigs to evaluate the possibility of establishing a suitable laboratory animal model to test and compare the safety on pregnancy of novel vaccine candidates against small ruminant brucellosis. Mid-term pregnant guinea pigs were inoculated subcutaneously with three different concentrations of the Rev.1 strain and euthanized at late-term gestation (>50 days). Blood samples were taken for sero-response before the pregnant guinea pigs were euthanized, and samples for bacteriology and histopathology were collected during necropsy. The Rev.1 strain was more consistently isolated from the spleen, chorioallantoic placentas and fetal organs of animals inoculated with ≥107 CFU of Rev.1 than from those inoculated with a lower dose. Histological alterations varied from mild to moderate presence of inflammatory cells in the spleen, mammary gland and pregnant uterus. In conclusion, placental colonization and vertical transmission were observed in pregnant guinea pigs after being inoculated subcutaneously at mid gestation with Rev.1, which is similar to what was reported in pregnant small ruminants vaccinated against brucellosis. Therefore, the pregnant guinea pig would be a useful model to initially asses the safety of vaccine candidates in pregnancy and compare them with the currently available commercial vaccine for brucellosis in small ruminants. Brucella melitensis es el agente etiológico de la brucelosis de los pequeños rumiantes, y el aborto es el signo clínico destacable. La vacunación con la cepa atenuada Rev.1 es la mejor opción para prevenir esa manifestación clínica y controlar la transmisión de la enfermedad. Sin embargo, la colonización del tracto genital es un efecto adverso que se observa tanto en esta como en otras cepas vacunales de Brucella. Los cobayos han demostrado ser un excelente modelo para evaluar la protección inmune y la eficacia de la vacuna Rev.1, pero no se han llevado a cabo estudios en esta especie para medir la seguridad de la vacuna sobre la preñez. El objetivo de este estudio fue caracterizar los efectos de la inoculación subcutánea (SC) de B. melitensis Rev.1 en cobayas gestantes, con el fin de establecer un modelo animal de laboratorio para evaluar y comparar la seguridad de candidatos vacunales contra la brucelosis de los pequeños rumiantes. Los animales fueron inoculados SC con tres diferentes concentraciones de Rev.1 y sacrificados al final de la gestación (>50 días). Para la necropsia se tomaron muestras para serología, bacteriología e histopatología. La cepa Rev.1 fue aislada de forma más consistente del bazo, membrana corioalantiodea y órganos fetales de aquellos animales inoculados con ≥107 UFC de Rev.1 que de los inoculados con una dosis más baja. Las alteraciones histológicas variaron de leve a moderada la presencia de células inflamatorias en el bazo, glándula mamaria y útero gestante. En conclusión, la colonización placentaria y la transmisión vertical fueron observadas en las cobayas preñadas luego de una inoculación subcutánea a mitad de la gestación, similar a lo observado en pequeños rumiantes vacunados contra la brucelosis. Por lo tanto, el modelo de cobaya gestante podria ser útil para evaluar la seguridad de los candidatos vacunales contra la brucelosis durante la preñez antes de testearlas en los hospedadores naturales. Instituto de Patobiología Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossi, Ursula Amaranta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Rossi, Ursula Amaranta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Castaño Zubieta, Mirta Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-08-21T14:54:29Z 2024-08-21T14:54:29Z 2024-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/19047 0325-8718 1669-2314 https://doi.org/10.58149/52b1-vw79 deu info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Ediciones INTA RIA 50 (2) : 100-109. (agosto 2024) |
| spellingShingle | Cobaya Vacunación Brucelosis Control de Enfermedades Pequeños Rumiantes Vacuna Guinea Pigs Vaccination Brucella melitensis Brucellosis Diseases Control Small Ruminants Vaccines Rossetti, Carlos Alberto Rossi, Ursula Amaranta Delgado, Fernando Oscar Castaño Zubieta, Mirta Raquel Foster, Camila Nayla Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title | Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title_full | Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title_fullStr | Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title_full_unstemmed | Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title_short | Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| title_sort | subcutaneous vaccination of pregnant guinea pigs with brucella melitensis rev 1 a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis |
| topic | Cobaya Vacunación Brucelosis Control de Enfermedades Pequeños Rumiantes Vacuna Guinea Pigs Vaccination Brucella melitensis Brucellosis Diseases Control Small Ruminants Vaccines |
| url | http://hdl.handle.net/20.500.12123/19047 https://doi.org/10.58149/52b1-vw79 |
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