Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immu...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18798 https://www.sciencedirect.com/science/article/pii/S0168170224001369 https://doi.org/10.1016/j.virusres.2024.199443 |
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| author | Hill, Holly Reddick, David Caspe, Sergio Gaston Ramage, Clifford Frew, David Rocchi, Mara S. Opriessnig, Tanja McNeilly, Tom Nathan |
| author_browse | Caspe, Sergio Gaston Frew, David Hill, Holly McNeilly, Tom Nathan Opriessnig, Tanja Ramage, Clifford Reddick, David Rocchi, Mara S. |
| author_facet | Hill, Holly Reddick, David Caspe, Sergio Gaston Ramage, Clifford Frew, David Rocchi, Mara S. Opriessnig, Tanja McNeilly, Tom Nathan |
| author_sort | Hill, Holly |
| collection | INTA Digital |
| description | Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18798 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA187982024-11-14T16:41:24Z Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs Hill, Holly Reddick, David Caspe, Sergio Gaston Ramage, Clifford Frew, David Rocchi, Mara S. Opriessnig, Tanja McNeilly, Tom Nathan Cerdo Virus Peste Porcina Reproducción Animal Enfermedades Respiratorias Swine Pestivirus Swine Fever Virus Animal Reproduction Respiratory Diseases Atypical Porcine Pestivirus Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology. EEA Mercedes Fil: Hill, Holly. Moredun Research Institute; Reino Unido Fil: Reddick, David. Moredun Scientific; Reino Unido Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido Fil: Ramage, Clifford. Moredun Scientific; Reino Unido Fil: Frew, David. Moredun Research Institute; Reino Unido Fil: Rocchi, Mara S. Moredun Research Institute; Reino Unido Fil: Opriessnig, Tanja. Moredun Research Institute; Reino Unido Fil: Opriessnig, Tanja. Iowa State University. College of Veterinary Medicine. Department of Veterinary Diagnostic and Production Animal Medicine; Estados Unidos Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido 2024-08-05T11:44:48Z 2024-08-05T11:44:48Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18798 https://www.sciencedirect.com/science/article/pii/S0168170224001369 0168-1702 https://doi.org/10.1016/j.virusres.2024.199443 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Virus Research 348 : 199443. (October 2024) |
| spellingShingle | Cerdo Virus Peste Porcina Reproducción Animal Enfermedades Respiratorias Swine Pestivirus Swine Fever Virus Animal Reproduction Respiratory Diseases Atypical Porcine Pestivirus Hill, Holly Reddick, David Caspe, Sergio Gaston Ramage, Clifford Frew, David Rocchi, Mara S. Opriessnig, Tanja McNeilly, Tom Nathan Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title | Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title_full | Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title_fullStr | Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title_full_unstemmed | Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title_short | Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| title_sort | enhancing the understanding of coinfection outcomes impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs |
| topic | Cerdo Virus Peste Porcina Reproducción Animal Enfermedades Respiratorias Swine Pestivirus Swine Fever Virus Animal Reproduction Respiratory Diseases Atypical Porcine Pestivirus |
| url | http://hdl.handle.net/20.500.12123/18798 https://www.sciencedirect.com/science/article/pii/S0168170224001369 https://doi.org/10.1016/j.virusres.2024.199443 |
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