First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina
The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only Sou...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18692 https://www.sciencedirect.com/science/article/abs/pii/S1090023324001436 https://doi.org/10.1016/j.tvjl.2024.106204 |
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| author | Olguin Perglione, Cecilia Politzki, Romina Paula Alvarez, Irene Ruiz, Vanesa |
| author_browse | Alvarez, Irene Olguin Perglione, Cecilia Politzki, Romina Paula Ruiz, Vanesa |
| author_facet | Olguin Perglione, Cecilia Politzki, Romina Paula Alvarez, Irene Ruiz, Vanesa |
| author_sort | Olguin Perglione, Cecilia |
| collection | INTA Digital |
| description | The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies.
None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18692 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA186922024-07-30T09:19:00Z First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina Olguin Perglione, Cecilia Politzki, Romina Paula Alvarez, Irene Ruiz, Vanesa Horses Animal Diseases Phylogeny Caballo Enfermedades de los Animales Parvoviridae Hepatitis Filogenia Argentina Equine Parvovirus-Hepatitis Serum Pools Parvovirus Equino Hepatitis The novel Equine Parvovirus-Hepatitis (EqPV-H) was first identified in the serum and liver of a horse that died of equine serum hepatitis, also known as Theiler's disease. Several reports in recent years strongly suggest that EqPV-H is the etiologic agent of Theiler's disease. Brazil is the only South American country where infection with this virus has been reported. This study investigated the presence of EqPV-H DNA in horse serum pools (n=51), commercial horse serum batches (n=5) and individual serum samples from donor horses (n=175) from Argentina. All serum samples were analyzed by quantitative polymerase chain reaction (qPCR) and samples with positive or indeterminate results were further analyzed by NS1 nested-PCR for phylogenetic studies. None of the serum pools was positive by qPCR but 9/51 pools were indeterminate (one or both test sample’s Ct values were higher than the limit of detection). The NS1 nested-PCR detected the EqPV-H DNA in 8 of these indeterminate samples (15.7 % of serum pools). Three of the commercial horse serum batches (60 %) contained EqPV-H DNA, detected either by qPCR and/or nested-PCR. From the 175 individual horse serum samples, three (1.71 %) were positive for EqPV-H by both techniques. The genetic analysis of the 12 partial NS1 sequences obtained showed that the local isolates were similar to EqPV-H sequences from Germany and China. This study provides the first evidence of the presence of EqPV-H in horses and in horse sera commercially available in Argentina and emphasizes the importance of controlling the biosecurity of commercial equine sera as well as any other blood-derived biological products of equine origin. Instituto de Virología Fil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Olguin Perglione, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Politzki, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Virología e Innovaciones Tecnológicas. Laboratorio de Virus Adventicios (IVIT); Argentina 2024-07-29T10:38:21Z 2024-07-29T10:38:21Z 2024-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18692 https://www.sciencedirect.com/science/article/abs/pii/S1090023324001436 1090-0233 https://doi.org/10.1016/j.tvjl.2024.106204 eng info:eu-repo/semantics/reference/hdl/20.500.12123/18696 info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Elsevier Veterinary journal 306 : 106204. (22 July 2024) |
| spellingShingle | Horses Animal Diseases Phylogeny Caballo Enfermedades de los Animales Parvoviridae Hepatitis Filogenia Argentina Equine Parvovirus-Hepatitis Serum Pools Parvovirus Equino Hepatitis Olguin Perglione, Cecilia Politzki, Romina Paula Alvarez, Irene Ruiz, Vanesa First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title | First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title_full | First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title_fullStr | First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title_full_unstemmed | First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title_short | First report of Equine Parvovirus-Hepatitis (EqPV-H) in Argentina |
| title_sort | first report of equine parvovirus hepatitis eqpv h in argentina |
| topic | Horses Animal Diseases Phylogeny Caballo Enfermedades de los Animales Parvoviridae Hepatitis Filogenia Argentina Equine Parvovirus-Hepatitis Serum Pools Parvovirus Equino Hepatitis |
| url | http://hdl.handle.net/20.500.12123/18692 https://www.sciencedirect.com/science/article/abs/pii/S1090023324001436 https://doi.org/10.1016/j.tvjl.2024.106204 |
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