Discovery of a resistance gene cluster associated with smut resistance in peanut
Peanut smut, caused by Thecaphora frezzii Carranza & J.C. Lindq., is an emerging threat for the global peanut industry. The disease’s destructive potential can be exemplified by pod incidence values as high as 70% and yield losses reaching 30%. Because fungicides have shown moderate but highly varia...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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American Peanut Research and Education Society
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/18691 https://peanutscience.com/article/id/1615/ https://doi.org/10.3146/0095-3679-51-PS23-6 |
| _version_ | 1855037897765289984 |
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| author | Chamberlin, Kelly D. Bennett, Rebecca S. Baldessari, Jorge Javier De La Barrera, Guillermo Cordes, Guillermo Grandon, Nancy Gabriela Mamani, Eva Maria Celia Rodriguez, Ana Valeria Morchetti, Sergio Holbrook, Corley C. Ozias-Akins, Peggy Ye, Chu Tallury, Shyam P. Clevenger, Josh Korani, W. Scheffler, Brian Youngblood, Ramey Callahan Simpson, Sheron |
| author_browse | Baldessari, Jorge Javier Bennett, Rebecca S. Chamberlin, Kelly D. Clevenger, Josh Cordes, Guillermo De La Barrera, Guillermo Grandon, Nancy Gabriela Holbrook, Corley C. Korani, W. Mamani, Eva Maria Celia Morchetti, Sergio Ozias-Akins, Peggy Rodriguez, Ana Valeria Scheffler, Brian Simpson, Sheron Tallury, Shyam P. Ye, Chu Youngblood, Ramey Callahan |
| author_facet | Chamberlin, Kelly D. Bennett, Rebecca S. Baldessari, Jorge Javier De La Barrera, Guillermo Cordes, Guillermo Grandon, Nancy Gabriela Mamani, Eva Maria Celia Rodriguez, Ana Valeria Morchetti, Sergio Holbrook, Corley C. Ozias-Akins, Peggy Ye, Chu Tallury, Shyam P. Clevenger, Josh Korani, W. Scheffler, Brian Youngblood, Ramey Callahan Simpson, Sheron |
| author_sort | Chamberlin, Kelly D. |
| collection | INTA Digital |
| description | Peanut smut, caused by Thecaphora frezzii Carranza & J.C. Lindq., is an emerging threat for the global peanut industry. The disease’s destructive potential can be exemplified by pod incidence values as high as 70% and yield losses reaching 30%. Because fungicides have shown moderate but highly variable levels of control levels, development and deployment of smut resistant cultivars are the best strategies for disease management. Screening for smut-resistant germplasm requires years of field trials and is currently the only option for breeders because genetic markers for resistance have not yet been developed. The objectives of this study were to perform whole genome sequencing (WGS) on populations developed for smut resistance mapping and subsequently fine map discovered QTL associated with smut resistance. An expedited strategy was employed by phenotyping in the F3 generation. We phenotyped 200 families with 3 plants per family for smut resistance in infested fields during the 2019/2020 season in General Deheza (32°45′23″S 63°47′20″W), Argentina. Each individual was sequenced using iGenomXRipTide library preparation and Illumina NovaSeq sequencing to yield approximately 1 times genome coverage. Analysis of the phenotype and genotype data using Khufu resulted in the identification of a single major smut resistance QTL on chromosome 12 (B02). Chromosome level genome sequences were assembled for the resistant parent (Ascasubi) and susceptible parent (Granoleico) using PacBio HIFI sequencing. A validation population was sequenced using Khufu to validate the QTL region and analysis confirmed the major locus on chromosome 12. The identified variation will be used to develop smut resistant varieties quickly using molecular assisted breeding strategies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18691 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | American Peanut Research and Education Society |
| publisherStr | American Peanut Research and Education Society |
| record_format | dspace |
| spelling | INTA186912024-07-29T10:55:05Z Discovery of a resistance gene cluster associated with smut resistance in peanut Chamberlin, Kelly D. Bennett, Rebecca S. Baldessari, Jorge Javier De La Barrera, Guillermo Cordes, Guillermo Grandon, Nancy Gabriela Mamani, Eva Maria Celia Rodriguez, Ana Valeria Morchetti, Sergio Holbrook, Corley C. Ozias-Akins, Peggy Ye, Chu Tallury, Shyam P. Clevenger, Josh Korani, W. Scheffler, Brian Youngblood, Ramey Callahan Simpson, Sheron Arachis hypogaea Cacahuete Carbón Groundnuts Smuts Plant Diseases Enfermedades de las Plantas Maní Carbón del Maní Peanuts Peanut smut, caused by Thecaphora frezzii Carranza & J.C. Lindq., is an emerging threat for the global peanut industry. The disease’s destructive potential can be exemplified by pod incidence values as high as 70% and yield losses reaching 30%. Because fungicides have shown moderate but highly variable levels of control levels, development and deployment of smut resistant cultivars are the best strategies for disease management. Screening for smut-resistant germplasm requires years of field trials and is currently the only option for breeders because genetic markers for resistance have not yet been developed. The objectives of this study were to perform whole genome sequencing (WGS) on populations developed for smut resistance mapping and subsequently fine map discovered QTL associated with smut resistance. An expedited strategy was employed by phenotyping in the F3 generation. We phenotyped 200 families with 3 plants per family for smut resistance in infested fields during the 2019/2020 season in General Deheza (32°45′23″S 63°47′20″W), Argentina. Each individual was sequenced using iGenomXRipTide library preparation and Illumina NovaSeq sequencing to yield approximately 1 times genome coverage. Analysis of the phenotype and genotype data using Khufu resulted in the identification of a single major smut resistance QTL on chromosome 12 (B02). Chromosome level genome sequences were assembled for the resistant parent (Ascasubi) and susceptible parent (Granoleico) using PacBio HIFI sequencing. A validation population was sequenced using Khufu to validate the QTL region and analysis confirmed the major locus on chromosome 12. The identified variation will be used to develop smut resistant varieties quickly using molecular assisted breeding strategies. EEA Manfredi Fil: Chamberlin, K.D. USDA. Agricultural Research Service. Wheat, Peanut and Other Field Crops Research Unit; Estados Unidos Fil: Bennetti, Rebecca S. USDA-ARS, Peanut and Small Grains Research Unit, Stillwater, Oklahoma Fil: Baldessari, Jorge Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: De la Barrera, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Cordes, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Grandon, Nancy Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Mamani, Eva María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Rodriguez, Ana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Morchetti, Sergio. Aceitera General Deheza; Argentina Fil: Holbrook, C.C. USDA-ARS. Crop Genetics and Breeding Research Unit; Estados Unidos Fil: Ozias-Akins, P. University of Georgia. Institute of Plant Breeding, Genetics, and Genomics. National Environmentally Sound Production Agriculture Laboratory; Estados Unidos Fil: Chu, Y. University of Georgia. Institute of Plant Breeding, Genetics, and Genomics. National Environmentally Sound Production Agriculture Laboratory; Estados Unidos Fil: Tallury, S. P. USDA-ARS. Plant Genetic Resources Conservation Unit; Estados Unidos Fil: Clevenger, J. HudsonAlpha Institute for Biotechnology; Estados Unidos Fil: Korani, W. HudsonAlpha Institute for Biotechnology; Estados Unidos Fil: Scheffler, B. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil. Youngblood, R. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Simpson, S. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos 2024-07-29T10:33:58Z 2024-07-29T10:33:58Z 2024-06-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18691 https://peanutscience.com/article/id/1615/ Chamberlin, K. y Bennett, R. y Baldessari, J. y De la Barrera, G. y Cordes, GG y Grandon, NG y Mamani, EM y Rodriquez, A. y Morchetti, S. y Holbrook, CC y Ozias-Akins, P. y Chu, Y. y Tallury, S. y Clevenger, J. y Korani, W. y Scheffler, B. y Youngblood, RC y Simpson, S. (2024) “Descubrimiento de un grupo de genes de resistencia asociado con la resistencia al carbón en el maní”, Peanut Science 51(1), págs. 59-65. doi: https://doi.org/10.3146/0095-3679-51-PS23-6 https://doi.org/10.3146/0095-3679-51-PS23-6 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Peanut Research and Education Society Peanut Science 51 (1) : 59-65. (2024) |
| spellingShingle | Arachis hypogaea Cacahuete Carbón Groundnuts Smuts Plant Diseases Enfermedades de las Plantas Maní Carbón del Maní Peanuts Chamberlin, Kelly D. Bennett, Rebecca S. Baldessari, Jorge Javier De La Barrera, Guillermo Cordes, Guillermo Grandon, Nancy Gabriela Mamani, Eva Maria Celia Rodriguez, Ana Valeria Morchetti, Sergio Holbrook, Corley C. Ozias-Akins, Peggy Ye, Chu Tallury, Shyam P. Clevenger, Josh Korani, W. Scheffler, Brian Youngblood, Ramey Callahan Simpson, Sheron Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title | Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title_full | Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title_fullStr | Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title_full_unstemmed | Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title_short | Discovery of a resistance gene cluster associated with smut resistance in peanut |
| title_sort | discovery of a resistance gene cluster associated with smut resistance in peanut |
| topic | Arachis hypogaea Cacahuete Carbón Groundnuts Smuts Plant Diseases Enfermedades de las Plantas Maní Carbón del Maní Peanuts |
| url | http://hdl.handle.net/20.500.12123/18691 https://peanutscience.com/article/id/1615/ https://doi.org/10.3146/0095-3679-51-PS23-6 |
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