Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle
A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After ini...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18583 https://www.sciencedirect.com/science/article/abs/pii/S0378113524001913 https://doi.org/10.1016/j.vetmic.2024.110169 |
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| author | Martinez, Mara Leila Esteban, Micaela Sanchez, Maria Cristina Saraullo, Vanina Rosa Hamer, Micaela Samartino, Luis Ernesto Brihuega, Bibiana Felicitas |
| author_browse | Brihuega, Bibiana Felicitas Esteban, Micaela Hamer, Micaela Martinez, Mara Leila Samartino, Luis Ernesto Sanchez, Maria Cristina Saraullo, Vanina Rosa |
| author_facet | Martinez, Mara Leila Esteban, Micaela Sanchez, Maria Cristina Saraullo, Vanina Rosa Hamer, Micaela Samartino, Luis Ernesto Brihuega, Bibiana Felicitas |
| author_sort | Martinez, Mara Leila |
| collection | INTA Digital |
| description | A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies were detected in 100% and 90% of the animals by IgG-ELISA and MAT, respectively. Both serological MAT and ELISA tests revealed interferences of vaccine antibodies. Disease diagnosis with ELISA and MAT methods should be made two and a half months and four months, respectively, after vaccination to avoid interference of vaccine antibodies. On the other hand, our results suggest that IgG-ELISA may be a useful method to assess the development of IgG antibodies induced by Leptospira vaccine. |
| format | Artículo |
| id | INTA18583 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA185832024-07-19T13:34:25Z Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle Martinez, Mara Leila Esteban, Micaela Sanchez, Maria Cristina Saraullo, Vanina Rosa Hamer, Micaela Samartino, Luis Ernesto Brihuega, Bibiana Felicitas Cattle ELISA Vaccines Leptospirosis Antibodies Immunodiagnosis Agglutination Tests Ganado Bovino Vacuna Anticuerpo Inmunodiagnóstico Reacciones de Aglutinación A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20. After initial vaccination, specific antibodies against Leptospira spp. were detected in 90% of the animals by IgG-ELISA and 60% by MAT, while after booster, antibodies were detected in 100% and 90% of the animals by IgG-ELISA and MAT, respectively. Both serological MAT and ELISA tests revealed interferences of vaccine antibodies. Disease diagnosis with ELISA and MAT methods should be made two and a half months and four months, respectively, after vaccination to avoid interference of vaccine antibodies. On the other hand, our results suggest that IgG-ELISA may be a useful method to assess the development of IgG antibodies induced by Leptospira vaccine. Instituto de Patobiología Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Samartino, Luis Ernesto. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2024-07-19T13:13:59Z 2024-07-19T13:13:59Z 2024 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18583 https://www.sciencedirect.com/science/article/abs/pii/S0378113524001913 0378-1135 https://doi.org/10.1016/j.vetmic.2024.110169 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario info:eu-repograntAgreement/INTA/2019-RIST-E6-I112-001, Red de Recursos Genéticos info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Microbiology : 110169 (Available online 9 July 2024) |
| spellingShingle | Cattle ELISA Vaccines Leptospirosis Antibodies Immunodiagnosis Agglutination Tests Ganado Bovino Vacuna Anticuerpo Inmunodiagnóstico Reacciones de Aglutinación Martinez, Mara Leila Esteban, Micaela Sanchez, Maria Cristina Saraullo, Vanina Rosa Hamer, Micaela Samartino, Luis Ernesto Brihuega, Bibiana Felicitas Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title | Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title_full | Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title_fullStr | Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title_full_unstemmed | Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title_short | Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| title_sort | study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle |
| topic | Cattle ELISA Vaccines Leptospirosis Antibodies Immunodiagnosis Agglutination Tests Ganado Bovino Vacuna Anticuerpo Inmunodiagnóstico Reacciones de Aglutinación |
| url | http://hdl.handle.net/20.500.12123/18583 https://www.sciencedirect.com/science/article/abs/pii/S0378113524001913 https://doi.org/10.1016/j.vetmic.2024.110169 |
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