Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion
The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/18557 https://www.sciencedirect.com/science/article/pii/S0929139323001828 https://doi.org/10.1016/j.apsoil.2023.104984 |
| _version_ | 1855037878315253760 |
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| author | Verdenelli, Romina A. Dominchin, María F. Barbero, Florencia Magali Perez Brandan, Carolina Aoki, Antonio Vargas Gil, Silvina Meriles, José Manuel |
| author_browse | Aoki, Antonio Barbero, Florencia Magali Dominchin, María F. Meriles, José Manuel Perez Brandan, Carolina Vargas Gil, Silvina Verdenelli, Romina A. |
| author_facet | Verdenelli, Romina A. Dominchin, María F. Barbero, Florencia Magali Perez Brandan, Carolina Aoki, Antonio Vargas Gil, Silvina Meriles, José Manuel |
| author_sort | Verdenelli, Romina A. |
| collection | INTA Digital |
| description | The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18557 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA185572025-05-07T12:56:26Z Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion Verdenelli, Romina A. Dominchin, María F. Barbero, Florencia Magali Perez Brandan, Carolina Aoki, Antonio Vargas Gil, Silvina Meriles, José Manuel Carbendazim Iprodione Enzyme Activity Water Erosion Fungicides Carbendazima Actividad Enzimática Erosión Hídrica Fungicida PLFA Microbial Abundance The impact of both fungicide and water erosion on soil microbial communities has been little studied, and far less in subhumid-dry climates. To help fill this knowledge gap, we evaluated the influence of two broad-spectrum fungicides applied to soil subjected to different degrees of water erosion on microbial population and function. Soil samples were taken from an experimental site under three water erosion levels: an agricultural land subjected to minimum water erosion by terrace farming (MIN), an agricultural land subjected to moderate water erosion without terrace farming (MOD), and a land with no water erosion control treatment (non-eroded, NON). Three dosages of both carbendazim and iprodione were applied to microcosms, and the responses of microbial community and function were examined after 30-day exposure. Phospholipid fatty acid (PLFA) analysis, soil enzyme activities, and microbial abundances estimated by quantitative real-time PCR (qPCR) were used to evaluate the impact of carbendazim and iprodione fungicides applied at three dosages on microbial community structure and enzyme activities. The lowest values of fluorescein diacetate hydrolase (FDA), dehydrogenase, urease, and glucosidase activities were registered in soil under moderate erosion, while the highest ones were observed in non-eroded soils. Both carbendazim and iprodione decreased C- and P-related activities, even when those fungicides were applied at field rate doses. Thus, phosphomonoesterase, glucosidase, and xylosidase in soil treated with fungicides applied at recommended field rate decreased by 46 %, 61 %, and 34 % compared to soil without fungicide application. The lowest values of PLFA biomarkers for Gram-positive bacteria, Gram-negative bacteria, and arbuscular mycorrhizal fungi were registered in soils treated with the highest dose of both carbendazim and iprodione. Thus, Gram-positive bacteria, Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungal PLFA biomarkers in soils without fungicide application were about 36 %, 3 %, 47 %, and 82 % higher than those soils treated with the highest doses of fungicides, respectively. In addition, the highest doses of fungicide application tended to greater decrease total PLFAs in both non-eroded and minimum eroded compared to moderate eroded soils. Similarly, the response of bacterial and fungal populations to fungicide treatment was more pronounced in non-eroded soils than in eroded soils. This study illustrates how the interactions between fungicides and water erosion levels affect soil enzyme activities, microbial abundances, and microbial community structure in agroecosystems. Instituto de Patología Vegetal Fil: Verdenelli, Romina A. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina Fil: Verdenelli, Romina A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Dominchin, María F. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina Fil: Dominchin, María F. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Barbero, Florencia M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina Fil: Barbero, Florencia M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aoki, Antonio. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Meriles, José M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Ciencia y Tecnología de Los Alimentos; Argentina Fil: Meriles, José M. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Multidisciplinario de Biología Vegetal; Argentina 2024-07-18T09:57:18Z 2024-07-18T09:57:18Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18557 https://www.sciencedirect.com/science/article/pii/S0929139323001828 0929-1393 1873-0272 (online) https://doi.org/10.1016/j.apsoil.2023.104984 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Applied Soil Ecology 190 : 104984 (October 2023) |
| spellingShingle | Carbendazim Iprodione Enzyme Activity Water Erosion Fungicides Carbendazima Actividad Enzimática Erosión Hídrica Fungicida PLFA Microbial Abundance Verdenelli, Romina A. Dominchin, María F. Barbero, Florencia Magali Perez Brandan, Carolina Aoki, Antonio Vargas Gil, Silvina Meriles, José Manuel Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title | Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title_full | Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title_fullStr | Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title_full_unstemmed | Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title_short | Effect of two broad-spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| title_sort | effect of two broad spectrum fungicides on the microbial communities of a soil subjected to different degrees of water erosion |
| topic | Carbendazim Iprodione Enzyme Activity Water Erosion Fungicides Carbendazima Actividad Enzimática Erosión Hídrica Fungicida PLFA Microbial Abundance |
| url | http://hdl.handle.net/20.500.12123/18557 https://www.sciencedirect.com/science/article/pii/S0929139323001828 https://doi.org/10.1016/j.apsoil.2023.104984 |
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