Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina
This study presents the results of the molecular detection of tick-borne microorganisms in Amblyomma tigrinum Koch collected near the city of Viedma, Río Negro, Argentina. Ticks were collected in their non-parasitic stage, on pet dogs and on Lycalopex gymnocercus (Pampa fox). Also, six tick samples...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer Link
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18534 https://link.springer.com/article/10.1007/s10493-023-00874-4 https://doi.org/10.1007/s10493-023-00874-4 |
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| author | Winter, Marina Sebastian, Patrick Tarragona, Evelina Luisa Flores, Fernando Sebastián Abate, Sergio Damián Nava, Santiago |
| author_browse | Abate, Sergio Damián Flores, Fernando Sebastián Nava, Santiago Sebastian, Patrick Tarragona, Evelina Luisa Winter, Marina |
| author_facet | Winter, Marina Sebastian, Patrick Tarragona, Evelina Luisa Flores, Fernando Sebastián Abate, Sergio Damián Nava, Santiago |
| author_sort | Winter, Marina |
| collection | INTA Digital |
| description | This study presents the results of the molecular detection of tick-borne microorganisms in Amblyomma tigrinum Koch collected near the city of Viedma, Río Negro, Argentina. Ticks were collected in their non-parasitic stage, on pet dogs and on Lycalopex gymnocercus (Pampa fox). Also, six tick samples from humans were analyzed. All ticks were morphologically identified to species level and genomic DNA was extracted. The DNA samples were examined by end point PCR assays to amplified DNA of Anaplasma sp., Babesia sp., Ehrlichia sp., Rickettsia sp. and Theileria sp. Although all tested DNA samples from the collected ticks resulted negative to the detection of Piroplasmida and Rickettsia spp., 16 samples (16.5%, including all hosts) were positive in the 16S rDNA gene PCR that detects bacteria from the Anaplasmataceae family. Phylogenetic analysis of seven obtained partial sequences resulted in the identification of three bacteria: two Ehrlichia spp. (related to Ehrlichia sp. strain Iberá and strain Viedma) and Candidatus Anaplasma boleense. The latter finding represents the first detection of this novel Candidatus species in A. tigrinum. Based on the results of this study, it must be assumed that the diversity of bacteria of the Anaplasmataceae family in Argentina is greater than previously thought, and that these bacteria can infect a wide range of domestic and wild animals. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18534 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer Link |
| publisherStr | Springer Link |
| record_format | dspace |
| spelling | INTA185342024-07-17T11:58:22Z Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina Winter, Marina Sebastian, Patrick Tarragona, Evelina Luisa Flores, Fernando Sebastián Abate, Sergio Damián Nava, Santiago Ehrlichia Wildlife South America Vida Silvestre América del Sur Amblyomma Ticks Garrapatas Anaplasma Amblyomma tigrinum Región Patagónica This study presents the results of the molecular detection of tick-borne microorganisms in Amblyomma tigrinum Koch collected near the city of Viedma, Río Negro, Argentina. Ticks were collected in their non-parasitic stage, on pet dogs and on Lycalopex gymnocercus (Pampa fox). Also, six tick samples from humans were analyzed. All ticks were morphologically identified to species level and genomic DNA was extracted. The DNA samples were examined by end point PCR assays to amplified DNA of Anaplasma sp., Babesia sp., Ehrlichia sp., Rickettsia sp. and Theileria sp. Although all tested DNA samples from the collected ticks resulted negative to the detection of Piroplasmida and Rickettsia spp., 16 samples (16.5%, including all hosts) were positive in the 16S rDNA gene PCR that detects bacteria from the Anaplasmataceae family. Phylogenetic analysis of seven obtained partial sequences resulted in the identification of three bacteria: two Ehrlichia spp. (related to Ehrlichia sp. strain Iberá and strain Viedma) and Candidatus Anaplasma boleense. The latter finding represents the first detection of this novel Candidatus species in A. tigrinum. Based on the results of this study, it must be assumed that the diversity of bacteria of the Anaplasmataceae family in Argentina is greater than previously thought, and that these bacteria can infect a wide range of domestic and wild animals. Universidad Nacional de Río Negro (PI-JI-40-C-869). Asociación Cooperadora INTA Rafaela. Agencia Nacional de Promoción Científica y Tecnológica (PICT 2019-970). EEA Rafaela Fil: Winter, Marina. Universidad Nacional de Río Negro. Centro de Investigaciones y Transferencia de Río Negro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Río Negro; Argentina Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT); Argentina Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT); Argentina Fil: Abate, Sergio Damian. Universidad Nacional de Río Negro. Centro de Investigaciones y Transferencia de Río Negro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Río Negro; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina 2024-07-17T11:43:39Z 2024-07-17T11:43:39Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18534 https://link.springer.com/article/10.1007/s10493-023-00874-4 1572-9702 (electronic) 0168-8162 https://doi.org/10.1007/s10493-023-00874-4 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Link Experimental and Applied Acarology 92 : 151-159 (January 2024) |
| spellingShingle | Ehrlichia Wildlife South America Vida Silvestre América del Sur Amblyomma Ticks Garrapatas Anaplasma Amblyomma tigrinum Región Patagónica Winter, Marina Sebastian, Patrick Tarragona, Evelina Luisa Flores, Fernando Sebastián Abate, Sergio Damián Nava, Santiago Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title | Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title_full | Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title_fullStr | Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title_full_unstemmed | Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title_short | Tick-borne microorganisms in Amblyomma tigrinum (Acari: Ixodidae) from the Patagonian region of Argentina |
| title_sort | tick borne microorganisms in amblyomma tigrinum acari ixodidae from the patagonian region of argentina |
| topic | Ehrlichia Wildlife South America Vida Silvestre América del Sur Amblyomma Ticks Garrapatas Anaplasma Amblyomma tigrinum Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/18534 https://link.springer.com/article/10.1007/s10493-023-00874-4 https://doi.org/10.1007/s10493-023-00874-4 |
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