Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus
RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific control of viruses including foot-and-mouth disease virus (FMDV), the causa...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Peer J Inc.
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18512 https://peerj.com/articles/11227/ https://doi.org/10.7717/peerj.11227 |
| _version_ | 1855037870620803072 |
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| author | Curra, Anabella Paola Cacciabue, Marco Polo Domingo Gravisaco, María José Asurmendi, Sebastian Taboga, Oscar Alberto Gismondi, Maria Ines |
| author_browse | Asurmendi, Sebastian Cacciabue, Marco Polo Domingo Curra, Anabella Paola Gismondi, Maria Ines Gravisaco, María José Taboga, Oscar Alberto |
| author_facet | Curra, Anabella Paola Cacciabue, Marco Polo Domingo Gravisaco, María José Asurmendi, Sebastian Taboga, Oscar Alberto Gismondi, Maria Ines |
| author_sort | Curra, Anabella Paola |
| collection | INTA Digital |
| description | RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific control of viruses including foot-and-mouth disease virus (FMDV), the causative agent of a devastating animal disease with high economic impact. The aim of this work was to assess the antiviral activity of different small RNA shuttles targeting the FMDV RNA-dependent RNA polymerase coding sequence (3D). Three target sequences were predicted within 3D considering RNA accessibility as a major criterion. The silencing efficacy of short-hairpin RNAs (shRNAs) and artificial microRNAs (amiRNAs) targeting the selected sequences was confirmed in fluorescent reporter assays. Furthermore, BHK-21 cells transiently expressing shRNAs or amiRNAs proved 70 to >95% inhibition of FMDV growth. Interestingly, dual expression of amiRNAs did not improve FMDV silencing. Lastly, stable cell lines constitutively expressing amiRNAs were established and characterized in terms of antiviral activity against FMDV. As expected, viral replication in these cell lines was delayed. These results show that the target RNA-accessibility-guided approach for RNAi design rendered efficient amiRNAs that constrain FMDV replication. The application of amiRNAs to complement FMDV vaccination in specific epidemiological scenarios shall be explored further. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18512 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Peer J Inc. |
| publisherStr | Peer J Inc. |
| record_format | dspace |
| spelling | INTA185122024-07-15T14:21:22Z Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus Curra, Anabella Paola Cacciabue, Marco Polo Domingo Gravisaco, María José Asurmendi, Sebastian Taboga, Oscar Alberto Gismondi, Maria Ines Foot-and-mouth Disease Aphthovirus Antiviral Agents RNA Fiebre Aftosa Virus Fiebre Aftosa Viricida ARN RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific control of viruses including foot-and-mouth disease virus (FMDV), the causative agent of a devastating animal disease with high economic impact. The aim of this work was to assess the antiviral activity of different small RNA shuttles targeting the FMDV RNA-dependent RNA polymerase coding sequence (3D). Three target sequences were predicted within 3D considering RNA accessibility as a major criterion. The silencing efficacy of short-hairpin RNAs (shRNAs) and artificial microRNAs (amiRNAs) targeting the selected sequences was confirmed in fluorescent reporter assays. Furthermore, BHK-21 cells transiently expressing shRNAs or amiRNAs proved 70 to >95% inhibition of FMDV growth. Interestingly, dual expression of amiRNAs did not improve FMDV silencing. Lastly, stable cell lines constitutively expressing amiRNAs were established and characterized in terms of antiviral activity against FMDV. As expected, viral replication in these cell lines was delayed. These results show that the target RNA-accessibility-guided approach for RNAi design rendered efficient amiRNAs that constrain FMDV replication. The application of amiRNAs to complement FMDV vaccination in specific epidemiological scenarios shall be explored further. Instituto de Biotecnología Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Curra, Anabella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cacciabue, Marco Polo Domingo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Cacciabue, Marco Polo Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Gravisaco, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-15T14:06:11Z 2024-07-15T14:06:11Z 2021-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18512 https://peerj.com/articles/11227/ 2167-8359 https://doi.org/10.7717/peerj.11227 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Peer J Inc. PeerJ 9 : e11227 (June 2021) |
| spellingShingle | Foot-and-mouth Disease Aphthovirus Antiviral Agents RNA Fiebre Aftosa Virus Fiebre Aftosa Viricida ARN Curra, Anabella Paola Cacciabue, Marco Polo Domingo Gravisaco, María José Asurmendi, Sebastian Taboga, Oscar Alberto Gismondi, Maria Ines Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title | Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title_full | Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title_fullStr | Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title_full_unstemmed | Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title_short | Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus |
| title_sort | antiviral efficacy of short hairpin rnas and artificial micrornas targeting foot and mouth disease virus |
| topic | Foot-and-mouth Disease Aphthovirus Antiviral Agents RNA Fiebre Aftosa Virus Fiebre Aftosa Viricida ARN |
| url | http://hdl.handle.net/20.500.12123/18512 https://peerj.com/articles/11227/ https://doi.org/10.7717/peerj.11227 |
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