Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis
Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributabl...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18504 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1359205/full https://doi.org/10.3389/fvets.2024.1359205 |
| _version_ | 1855486185766387712 |
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| author | Encinas, Micaela Ferrara Muñiz, Ximena Sammarruco, Romina Ayelén Ruiz Menna, Victoria Aymara Garro, Carlos Javier Delgado, Fernando Oscar Macías, Analía Magnano, Gabriel Zumarraga, Martin Jose Garbaccio, Sergio Gabriel Eirin, Maria Emilia |
| author_browse | Delgado, Fernando Oscar Eirin, Maria Emilia Encinas, Micaela Ferrara Muñiz, Ximena Garbaccio, Sergio Gabriel Garro, Carlos Javier Macías, Analía Magnano, Gabriel Ruiz Menna, Victoria Aymara Sammarruco, Romina Ayelén Zumarraga, Martin Jose |
| author_facet | Encinas, Micaela Ferrara Muñiz, Ximena Sammarruco, Romina Ayelén Ruiz Menna, Victoria Aymara Garro, Carlos Javier Delgado, Fernando Oscar Macías, Analía Magnano, Gabriel Zumarraga, Martin Jose Garbaccio, Sergio Gabriel Eirin, Maria Emilia |
| author_sort | Encinas, Micaela |
| collection | INTA Digital |
| description | Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case–control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds. |
| format | Artículo |
| id | INTA18504 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA185042024-07-15T12:19:08Z Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis Encinas, Micaela Ferrara Muñiz, Ximena Sammarruco, Romina Ayelén Ruiz Menna, Victoria Aymara Garro, Carlos Javier Delgado, Fernando Oscar Macías, Analía Magnano, Gabriel Zumarraga, Martin Jose Garbaccio, Sergio Gabriel Eirin, Maria Emilia Bovine Tuberculosis Diagnosis Blood Tuberculin PCR Cattle Tuberculosis Bovina Diagnóstico Sangre Tuberculina Ganado Bovino Mycobacterium bovis False-negative Falso-negativo Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case–control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis. Detection of the IS6110 target through touchdown blood-PCR (IS6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases (p = 0.134). Most cases identified by the IS6110 TD-PCR exhibited focalized lesions (p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis, further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds. Instituto de Biotecnología Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Encinas, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Sammarruco, Romina Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruiz Menna, Victoria Aymara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Ruiz Menna, Victoria Aymara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Delgado, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-15T10:50:29Z 2024-07-15T10:50:29Z 2024-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18504 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1359205/full 2297-1769 https://doi.org/10.3389/fvets.2024.1359205 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 11 : 1359205 (May 2024) |
| spellingShingle | Bovine Tuberculosis Diagnosis Blood Tuberculin PCR Cattle Tuberculosis Bovina Diagnóstico Sangre Tuberculina Ganado Bovino Mycobacterium bovis False-negative Falso-negativo Encinas, Micaela Ferrara Muñiz, Ximena Sammarruco, Romina Ayelén Ruiz Menna, Victoria Aymara Garro, Carlos Javier Delgado, Fernando Oscar Macías, Analía Magnano, Gabriel Zumarraga, Martin Jose Garbaccio, Sergio Gabriel Eirin, Maria Emilia Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title | Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title_full | Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title_fullStr | Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title_full_unstemmed | Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title_short | Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis |
| title_sort | limited usefulness of the is6110 touchdown pcr in blood for tuberculin skin test false negative cattle with serological response to mycobacterium bovis |
| topic | Bovine Tuberculosis Diagnosis Blood Tuberculin PCR Cattle Tuberculosis Bovina Diagnóstico Sangre Tuberculina Ganado Bovino Mycobacterium bovis False-negative Falso-negativo |
| url | http://hdl.handle.net/20.500.12123/18504 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1359205/full https://doi.org/10.3389/fvets.2024.1359205 |
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