Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology
Wastewater-based epidemiology (WBE) has gained prominence worldwide as a powerful tool in public health. This study aimed to monitor the circulation of Hepatitis E Virus (HEV) from wastewater samples collected during a six-year period and compare these results with clinical surveillance in the centr...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/18503 https://www.sciencedirect.com/science/article/abs/pii/S0043135424009047 https://doi.org/10.1016/j.watres.2024.122004 |
| _version_ | 1855486185570304000 |
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| author | Fantilli, Anabella Clara Masachessi, Gisela Di Cola, Guadalupe Castro, Gonzalo Sicilia, Paola Marinzalda, María de los Angeles Cachi, Ariana Mariela Moreno, Claudia Borda, Mariel Ibarra, Gustavo Rojas, Ricardo Manuel Parreño, Gladys Viviana Barbás, María Gabriela Nates, Silvia Viviana Pisano, María Belén Ré, Viviana Elizabeth |
| author_browse | Barbás, María Gabriela Borda, Mariel Cachi, Ariana Mariela Castro, Gonzalo Di Cola, Guadalupe Fantilli, Anabella Clara Ibarra, Gustavo Marinzalda, María de los Angeles Masachessi, Gisela Moreno, Claudia Nates, Silvia Viviana Parreño, Gladys Viviana Pisano, María Belén Rojas, Ricardo Manuel Ré, Viviana Elizabeth Sicilia, Paola |
| author_facet | Fantilli, Anabella Clara Masachessi, Gisela Di Cola, Guadalupe Castro, Gonzalo Sicilia, Paola Marinzalda, María de los Angeles Cachi, Ariana Mariela Moreno, Claudia Borda, Mariel Ibarra, Gustavo Rojas, Ricardo Manuel Parreño, Gladys Viviana Barbás, María Gabriela Nates, Silvia Viviana Pisano, María Belén Ré, Viviana Elizabeth |
| author_sort | Fantilli, Anabella Clara |
| collection | INTA Digital |
| description | Wastewater-based epidemiology (WBE) has gained prominence worldwide as a powerful tool in public health. This study aimed to monitor the circulation of Hepatitis E Virus (HEV) from wastewater samples collected during a six-year period and compare these results with clinical surveillance in the central region of Argentina. From 2017 to 2022, 1008 raw wastewater samples were analyzed, including four wastewater treatment plants from four cities (n=319), and 7 local neighborhood collector sewers in Córdoba city (n=689). Serum and/or stool samples from patients suspected of HEV infection were also analyzed (n=48). HEV molecular detection and viral load quantification were performed by real time RT-qPCR, and genetic characterization by two RT-Nested PCRs (targeting partial ORF-1 and ORF-2 genomic regions), sequencing and phylogenetic analysis.
Fifty-three (5.3%) wastewater samples were RNA-HEV positive by real time RT-qPCR, with variations according to the location and year (0.0% - 21.6%). Out of these, ORF-2 genomic region was amplified in 20 samples (37.7%) and ORF-1 partial region in 12 (22.6%), and eighteen sequences were obtained. Throughout the study period, two (4.2%) HEV confirmed infections were reported, and one sequence was obtained. Phylogenetic analyses for both genomic regions showed that all the isolates were genotype HEV-3 clade abchijklm.
Our study detected HEV in wastewater over a six-year period, despite a low number of clinical cases, emphasizing WBE as a valuable tool that complements clinical surveillance, by detecting pathogens' presence; identifying their transmission, circulation dynamics and excretion hotspots; and revealing changes in their genomic diversity. |
| format | Artículo |
| id | INTA18503 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA185032024-07-15T12:18:16Z Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology Fantilli, Anabella Clara Masachessi, Gisela Di Cola, Guadalupe Castro, Gonzalo Sicilia, Paola Marinzalda, María de los Angeles Cachi, Ariana Mariela Moreno, Claudia Borda, Mariel Ibarra, Gustavo Rojas, Ricardo Manuel Parreño, Gladys Viviana Barbás, María Gabriela Nates, Silvia Viviana Pisano, María Belén Ré, Viviana Elizabeth Hepatitis Wastewater Epidemiology Surveillance Systems Agua Residual Epidemiología Sistemas de Vigilancia Argentina Hepatitis E Virus Virus Hepatitis E Wastewater-based epidemiology (WBE) has gained prominence worldwide as a powerful tool in public health. This study aimed to monitor the circulation of Hepatitis E Virus (HEV) from wastewater samples collected during a six-year period and compare these results with clinical surveillance in the central region of Argentina. From 2017 to 2022, 1008 raw wastewater samples were analyzed, including four wastewater treatment plants from four cities (n=319), and 7 local neighborhood collector sewers in Córdoba city (n=689). Serum and/or stool samples from patients suspected of HEV infection were also analyzed (n=48). HEV molecular detection and viral load quantification were performed by real time RT-qPCR, and genetic characterization by two RT-Nested PCRs (targeting partial ORF-1 and ORF-2 genomic regions), sequencing and phylogenetic analysis. Fifty-three (5.3%) wastewater samples were RNA-HEV positive by real time RT-qPCR, with variations according to the location and year (0.0% - 21.6%). Out of these, ORF-2 genomic region was amplified in 20 samples (37.7%) and ORF-1 partial region in 12 (22.6%), and eighteen sequences were obtained. Throughout the study period, two (4.2%) HEV confirmed infections were reported, and one sequence was obtained. Phylogenetic analyses for both genomic regions showed that all the isolates were genotype HEV-3 clade abchijklm. Our study detected HEV in wastewater over a six-year period, despite a low number of clinical cases, emphasizing WBE as a valuable tool that complements clinical surveillance, by detecting pathogens' presence; identifying their transmission, circulation dynamics and excretion hotspots; and revealing changes in their genomic diversity. Instituto de Virología Fil: Fantilli, Anabella Clara. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Fantilli, Anabella Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Masachessi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Cola, Guadalupe. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Di Cola, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castro, Gonzalo. Ministerio de Salud de la Provincia de Córdoba. Departamento Laboratorio Central; Argentina Fil: Sicilia, Paola. Ministerio de Salud de la Provincia de Córdoba. Departamento Laboratorio Central; Argentina Fil: Marinzalda, María de los Angeles. Universidad de la Defensa Nacional. Facultad de la Fuerza Aérea. Instituto Nacional de Medicina Aeronáutica y Espacial; Argentina Fil: Marinzalda, María de los Angeles. Planta Municipal de tratamiento de efluente cloacales Bajo Grande. Laboratorio de análisis fisicoquímicos, bacteriológicos EDAR Bajo Grande; Argentina Fil: Cachi, Ariana Mariela. Universidad de la Defensa Nacional. Facultad de la Fuerza Aérea. Instituto Nacional de Medicina Aeronáutica y Espacial; Argentina Fil: Cachi, Ariana Mariela. Planta Municipal de tratamiento de efluente cloacales Bajo Grande. Laboratorio de análisis fisicoquímicos, bacteriológicos EDAR Bajo Grande; Argentina Fil: Moreno, Claudia. Ministerio de Salud de la Provincia de Córdoba. Departamento Laboratorio Central; Argentina Fil: Borda, Mariel. Ministerio de Salud de la Provincia de Córdoba. Departamento Laboratorio Central; Argentina Fil: Ibarra, Gustavo. Planta Municipal de tratamiento de efluente cloacales Bajo Grande. Laboratorio de análisis fisicoquímicos, bacteriológicos EDAR Bajo Grande; Argentina Fil: Rojas, Ricardo Manuel. Cooperativa Integral Regional de Provisión de Servicios Públicos, Vivienda y Consumo Limitada (COOPI); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Barbás, María Gabriela. Ministerio de Salud de la Provincia de Córdoba. Departamento Laboratorio Central; Argentina Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Pisano, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología “Dr. J. M. Vanella”; Argentina Fil: Ré, Viviana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-15T10:22:45Z 2024-07-15T10:22:45Z 2024-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18503 https://www.sciencedirect.com/science/article/abs/pii/S0043135424009047 1879-2448 https://doi.org/10.1016/j.watres.2024.122004 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Water Research 261 : 122004 (Septiembre 2024) |
| spellingShingle | Hepatitis Wastewater Epidemiology Surveillance Systems Agua Residual Epidemiología Sistemas de Vigilancia Argentina Hepatitis E Virus Virus Hepatitis E Fantilli, Anabella Clara Masachessi, Gisela Di Cola, Guadalupe Castro, Gonzalo Sicilia, Paola Marinzalda, María de los Angeles Cachi, Ariana Mariela Moreno, Claudia Borda, Mariel Ibarra, Gustavo Rojas, Ricardo Manuel Parreño, Gladys Viviana Barbás, María Gabriela Nates, Silvia Viviana Pisano, María Belén Ré, Viviana Elizabeth Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title | Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title_full | Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title_fullStr | Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title_full_unstemmed | Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title_short | Integrated hepatitis e virus monitoring in central Argentina : a six-year analysis of clinical surveillance and wastewater-based epidemiology |
| title_sort | integrated hepatitis e virus monitoring in central argentina a six year analysis of clinical surveillance and wastewater based epidemiology |
| topic | Hepatitis Wastewater Epidemiology Surveillance Systems Agua Residual Epidemiología Sistemas de Vigilancia Argentina Hepatitis E Virus Virus Hepatitis E |
| url | http://hdl.handle.net/20.500.12123/18503 https://www.sciencedirect.com/science/article/abs/pii/S0043135424009047 https://doi.org/10.1016/j.watres.2024.122004 |
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