Transmissibility of Mycobacterium pinnipedii in a murine model
The causative agent of tuberculosis in pinnipeds is Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex (MTC). The natural hosts are pinnipeds; however, other non-marine mammals, including humans, can also be infected. The transmissibility of a pathogen is related to its vir...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Frontiers Media
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18458 https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1328981/full https://doi.org/10.3389/fcimb.2024.1328981 |
| _version_ | 1855486175966396416 |
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| author | Marfil, Maria Jimena Blanco, Federico Carlos Colombatti Olivieri, Maria Alejandra Eirin, Maria Emilia Zumarraga, Martin Jose |
| author_browse | Blanco, Federico Carlos Colombatti Olivieri, Maria Alejandra Eirin, Maria Emilia Marfil, Maria Jimena Zumarraga, Martin Jose |
| author_facet | Marfil, Maria Jimena Blanco, Federico Carlos Colombatti Olivieri, Maria Alejandra Eirin, Maria Emilia Zumarraga, Martin Jose |
| author_sort | Marfil, Maria Jimena |
| collection | INTA Digital |
| description | The causative agent of tuberculosis in pinnipeds is Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex (MTC). The natural hosts are pinnipeds; however, other non-marine mammals, including humans, can also be infected. The transmissibility of a pathogen is related to its virulence. The transmissibility of a M. pinnipedii strain (i.e., 1856) was investigated in a murine model and compared with that of two Mycobacterium bovis strains (i.e., 534 and 04-303) with different reported virulence. Non-inoculated mice (sentinels) were co-housed with intratracheally inoculated mice. Detailed inspection of mice to search for visible tuberculosis lesions in the lungs and spleen was performed, and bacillus viability at 30, 60, and 90 days post-inoculation (dpi) was assayed. A transmissibility of 100% was recorded at 30 dpi in sentinel mice co-housed with the inoculated mice from the M. pinnipedii and M. bovis 04-303 groups, as evidenced by the recovery of viable M. pinnipedii and M. bovis from the lungs of sentinel mice. Mice inoculated with M. pinnipedii (1856) and M. bovis (534) survived until euthanized, whereas five of the M. bovis 04-303-inoculated mice died at 17 dpi. This study constitutes the first report of the transmissibility of a M. pinnipedii strain in mice and confirms the utility of this experimental model to study virulence features such as the transmission of poorly characterized MTC species. |
| format | Artículo |
| id | INTA18458 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA184582024-07-11T10:32:34Z Transmissibility of Mycobacterium pinnipedii in a murine model Marfil, Maria Jimena Blanco, Federico Carlos Colombatti Olivieri, Maria Alejandra Eirin, Maria Emilia Zumarraga, Martin Jose Mycobacterium bovis Mycobacterium tuberculosis Mycobacterium Mycobacterium pinnipedii Modelo Murino Murine Model The causative agent of tuberculosis in pinnipeds is Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex (MTC). The natural hosts are pinnipeds; however, other non-marine mammals, including humans, can also be infected. The transmissibility of a pathogen is related to its virulence. The transmissibility of a M. pinnipedii strain (i.e., 1856) was investigated in a murine model and compared with that of two Mycobacterium bovis strains (i.e., 534 and 04-303) with different reported virulence. Non-inoculated mice (sentinels) were co-housed with intratracheally inoculated mice. Detailed inspection of mice to search for visible tuberculosis lesions in the lungs and spleen was performed, and bacillus viability at 30, 60, and 90 days post-inoculation (dpi) was assayed. A transmissibility of 100% was recorded at 30 dpi in sentinel mice co-housed with the inoculated mice from the M. pinnipedii and M. bovis 04-303 groups, as evidenced by the recovery of viable M. pinnipedii and M. bovis from the lungs of sentinel mice. Mice inoculated with M. pinnipedii (1856) and M. bovis (534) survived until euthanized, whereas five of the M. bovis 04-303-inoculated mice died at 17 dpi. This study constitutes the first report of the transmissibility of a M. pinnipedii strain in mice and confirms the utility of this experimental model to study virulence features such as the transmission of poorly characterized MTC species. Instituto de Biotecnología Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-11T10:22:21Z 2024-07-11T10:22:21Z 2024-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18458 https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1328981/full 2235-2988 https://doi.org/10.3389/fcimb.2024.1328981 eng info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Cellular and Infection Microbiology 14 : 1328981 (March 2024) |
| spellingShingle | Mycobacterium bovis Mycobacterium tuberculosis Mycobacterium Mycobacterium pinnipedii Modelo Murino Murine Model Marfil, Maria Jimena Blanco, Federico Carlos Colombatti Olivieri, Maria Alejandra Eirin, Maria Emilia Zumarraga, Martin Jose Transmissibility of Mycobacterium pinnipedii in a murine model |
| title | Transmissibility of Mycobacterium pinnipedii in a murine model |
| title_full | Transmissibility of Mycobacterium pinnipedii in a murine model |
| title_fullStr | Transmissibility of Mycobacterium pinnipedii in a murine model |
| title_full_unstemmed | Transmissibility of Mycobacterium pinnipedii in a murine model |
| title_short | Transmissibility of Mycobacterium pinnipedii in a murine model |
| title_sort | transmissibility of mycobacterium pinnipedii in a murine model |
| topic | Mycobacterium bovis Mycobacterium tuberculosis Mycobacterium Mycobacterium pinnipedii Modelo Murino Murine Model |
| url | http://hdl.handle.net/20.500.12123/18458 https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1328981/full https://doi.org/10.3389/fcimb.2024.1328981 |
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