Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances
Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, A...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/18404 https://www.sciencedirect.com/science/article/abs/pii/S0048969724033217 https://doi.org/10.1016/j.scitotenv.2024.173174 |
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| author | Bahl, María Florencia Salgado Costa, Carolina Demetrio, Pablo Martín Mac Loughlin, Tomás Mariano Arruti, María Emilia Brodeur, Celine Marie Natale, Guillermo Sebastián |
| author_browse | Arruti, María Emilia Bahl, María Florencia Brodeur, Celine Marie Demetrio, Pablo Martín Mac Loughlin, Tomás Mariano Natale, Guillermo Sebastián Salgado Costa, Carolina |
| author_facet | Bahl, María Florencia Salgado Costa, Carolina Demetrio, Pablo Martín Mac Loughlin, Tomás Mariano Arruti, María Emilia Brodeur, Celine Marie Natale, Guillermo Sebastián |
| author_sort | Bahl, María Florencia |
| collection | INTA Digital |
| description | Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined. |
| format | Artículo |
| id | INTA18404 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA184042024-07-05T14:59:08Z Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances Bahl, María Florencia Salgado Costa, Carolina Demetrio, Pablo Martín Mac Loughlin, Tomás Mariano Arruti, María Emilia Brodeur, Celine Marie Natale, Guillermo Sebastián Rana Anfibio Contaminación Medio Ambiente Frogs Amphibians Contamination Environment Biomarcadores Biomarkers Leptodactylus luctator Amphibians are the most threatened group of vertebrates because they have certain biological and ecological characteristics that make them sensitive to environmental changes. The aim of this study was to evaluate the health status of field-collected adult frogs of Leptodactylus luctator (Amphibia, Anura) living in sites with different anthropogenic disturbances (florihorticulture, petrochemical industry and sewage discharges) and a reference site without any detectable influence of such activities. To this end, a battery of 21 biomarkers (hematological, biochemical and individual biomarkers) was studied using a multivariate approach that allows us to evaluate the relationship between them and provide information on their usefulness. The frogs at the florihorticulture, petrochemical and sewage discharges sites exhibited several biomarkers far from homeostasis. In addition, we identified 11 of 21 biomarkers that were useful indicators of the health status of the frogs and allowed discrimination between study sites in the following order: lymphocytes (98 %), neutrophils (45 %), hemoglobin (42 %), monocytes (41 %), fat body index (35 %), eosinophils (35 %), hepatosomatic index (33 %), mean corpuscular hemoglobin (32 %), thrombocytes (27 %), catalase in liver (26 %), and GST in liver (26 %). The results suggest that hematological biomarkers contribute the most to site separation, whereas biochemical biomarkers contribute the least. The integral interpretation of the results also allowed us to diagnose the different health status of L. luctator: The frogs from the petrochemical industry were the most negatively affected, followed by the frogs from the sewages discharges and finally the frogs from the florihorticulture and reference sites. This is the first field study with anurans in which so many biomarkers were examined. Instituto de Recursos Biológicos Fil: Bahl, M.F. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina Fil: Salgado Costa, C. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina Fil: Demetrio, P.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina Fil: Mac Loughlin, T.M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina Fil: Arruti, M.E. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Natale, G.S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente (CIM); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones del Medio Ambiente (CIM); Argentina 2024-07-05T13:52:30Z 2024-07-05T13:52:30Z 2024-07-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18404 https://www.sciencedirect.com/science/article/abs/pii/S0048969724033217 0048-9697 https://doi.org/10.1016/j.scitotenv.2024.173174 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Science of The Total Environment 933 : 173174. (July 2024) |
| spellingShingle | Rana Anfibio Contaminación Medio Ambiente Frogs Amphibians Contamination Environment Biomarcadores Biomarkers Leptodactylus luctator Bahl, María Florencia Salgado Costa, Carolina Demetrio, Pablo Martín Mac Loughlin, Tomás Mariano Arruti, María Emilia Brodeur, Celine Marie Natale, Guillermo Sebastián Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title | Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title_full | Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title_fullStr | Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title_full_unstemmed | Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title_short | Integration of a battery of biomarkers to evaluate the health status of field-collected frogs of Leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| title_sort | integration of a battery of biomarkers to evaluate the health status of field collected frogs of leptodactylus luctator living in ecosystems with different anthropogenic disturbances |
| topic | Rana Anfibio Contaminación Medio Ambiente Frogs Amphibians Contamination Environment Biomarcadores Biomarkers Leptodactylus luctator |
| url | http://hdl.handle.net/20.500.12123/18404 https://www.sciencedirect.com/science/article/abs/pii/S0048969724033217 https://doi.org/10.1016/j.scitotenv.2024.173174 |
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