Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes
Snakin/GASA proteins share 12 cysteines in conserved positions in the C-terminal region. Most of them were involved in different aspects of plant growth and development, while a small number of these peptides were reported to have antimicrobial activity or participate in abiotic stress tolerance. In...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18364 https://link.springer.com/article/10.1007/s00299-023-03108-4 https://doi.org/10.1007/s00299-023-03108-4 |
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| author | Nahirñak, Vanesa Almasia, Natalia Ines Lia, Veronica Viviana Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Hopp, Horacio Esteban Lia, Veronica Viviana Nahirñak, Vanesa Vazquez Rovere, Cecilia |
| author_facet | Nahirñak, Vanesa Almasia, Natalia Ines Lia, Veronica Viviana Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_sort | Nahirñak, Vanesa |
| collection | INTA Digital |
| description | Snakin/GASA proteins share 12 cysteines in conserved positions in the C-terminal region. Most of them were involved in different aspects of plant growth and development, while a small number of these peptides were reported to have antimicrobial activity or participate in abiotic stress tolerance. In potato, 18 Snakin/GASA genes were identified and classified into three groups based on phylogenetic analysis. Snakin-1 and Snakin-2 are members of subfamilies I and II, respectively, and were reported to be implicated not only in defense against pathogens but also in plant development. In this work, we present the first in-depth characterization of Snakin-3, a member of the subfamily III within the Snakin/GASA gene family of potato. Transient co-expression of Snakin-3 fused to the green fluorescent protein and organelle markers revealed that it is located in the endoplasmic reticulum. Furthermore, expression analyses via pSnakin-3::GUS transgenic plants showed GUS staining mainly in roots and vascular tissues of the stem. Moreover, GUS expression levels were increased after inoculation with Pseudomonas syringae pv. tabaci or Pectobacterium carotovorum subsp. carotovorum and also after auxin treatment mainly in roots and stems. To gain further insights into the function of Snakin-3 in planta, potato overexpressing lines were challenged against P. carotovorum subsp. carotovorum showing enhanced tolerance to this bacterial pathogen. In sum, here we report the first functional characterization of a Snakin/GASA gene from subfamily III in Solanaceae. Our findings provide experimental evidence on promoter activity and subcellular localization and reveal a role of potato Snakin-3 in plant defense. |
| format | Artículo |
| id | INTA18364 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA183642024-07-03T09:56:07Z Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes Nahirñak, Vanesa Almasia, Natalia Ines Lia, Veronica Viviana Hopp, Horacio Esteban Vazquez Rovere, Cecilia Potatoes Defence Mechanisms Antimicrobials Disease Resistance Peptides Papa Mecanismos de Defensa Antimicrobianos Resistencia a la Enfermedad Péptidos Solanum tuberosum Snakin-3 Snakin/GASA Snakin/GASA proteins share 12 cysteines in conserved positions in the C-terminal region. Most of them were involved in different aspects of plant growth and development, while a small number of these peptides were reported to have antimicrobial activity or participate in abiotic stress tolerance. In potato, 18 Snakin/GASA genes were identified and classified into three groups based on phylogenetic analysis. Snakin-1 and Snakin-2 are members of subfamilies I and II, respectively, and were reported to be implicated not only in defense against pathogens but also in plant development. In this work, we present the first in-depth characterization of Snakin-3, a member of the subfamily III within the Snakin/GASA gene family of potato. Transient co-expression of Snakin-3 fused to the green fluorescent protein and organelle markers revealed that it is located in the endoplasmic reticulum. Furthermore, expression analyses via pSnakin-3::GUS transgenic plants showed GUS staining mainly in roots and vascular tissues of the stem. Moreover, GUS expression levels were increased after inoculation with Pseudomonas syringae pv. tabaci or Pectobacterium carotovorum subsp. carotovorum and also after auxin treatment mainly in roots and stems. To gain further insights into the function of Snakin-3 in planta, potato overexpressing lines were challenged against P. carotovorum subsp. carotovorum showing enhanced tolerance to this bacterial pathogen. In sum, here we report the first functional characterization of a Snakin/GASA gene from subfamily III in Solanaceae. Our findings provide experimental evidence on promoter activity and subcellular localization and reveal a role of potato Snakin-3 in plant defense. Instituto de Biotecnología Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lia, Veronica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-03T09:51:00Z 2024-07-03T09:51:00Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18364 https://link.springer.com/article/10.1007/s00299-023-03108-4 1432-203X https://doi.org/10.1007/s00299-023-03108-4 eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001, Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Plant Cell Reports 43 (2) : 47 (February 2024) |
| spellingShingle | Potatoes Defence Mechanisms Antimicrobials Disease Resistance Peptides Papa Mecanismos de Defensa Antimicrobianos Resistencia a la Enfermedad Péptidos Solanum tuberosum Snakin-3 Snakin/GASA Nahirñak, Vanesa Almasia, Natalia Ines Lia, Veronica Viviana Hopp, Horacio Esteban Vazquez Rovere, Cecilia Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title | Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title_full | Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title_fullStr | Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title_full_unstemmed | Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title_short | Unveiling the defensive role of Snakin‑3, a member of the subfamily III of Snakin/GASA peptides in potatoes |
| title_sort | unveiling the defensive role of snakin 3 a member of the subfamily iii of snakin gasa peptides in potatoes |
| topic | Potatoes Defence Mechanisms Antimicrobials Disease Resistance Peptides Papa Mecanismos de Defensa Antimicrobianos Resistencia a la Enfermedad Péptidos Solanum tuberosum Snakin-3 Snakin/GASA |
| url | http://hdl.handle.net/20.500.12123/18364 https://link.springer.com/article/10.1007/s00299-023-03108-4 https://doi.org/10.1007/s00299-023-03108-4 |
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