Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina
Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamic...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18362 https://link.springer.com/article/10.1007/s00705-024-06028-0 https://doi.org/10.1007/s00705-024-06028-0 |
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| author | Lozano Calderon, Laura Camila Cabanne, Gustavo Sebastián Marcos, Andrea Galdo Novo, Sabrina Torres, Carolina Perez, Andres Pybus, Oliver G. Konig, Guido Alberto |
| author_browse | Cabanne, Gustavo Sebastián Galdo Novo, Sabrina Konig, Guido Alberto Lozano Calderon, Laura Camila Marcos, Andrea Perez, Andres Pybus, Oliver G. Torres, Carolina |
| author_facet | Lozano Calderon, Laura Camila Cabanne, Gustavo Sebastián Marcos, Andrea Galdo Novo, Sabrina Torres, Carolina Perez, Andres Pybus, Oliver G. Konig, Guido Alberto |
| author_sort | Lozano Calderon, Laura Camila |
| collection | INTA Digital |
| description | Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18362 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA183622024-07-03T09:37:23Z Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina Lozano Calderon, Laura Camila Cabanne, Gustavo Sebastián Marcos, Andrea Galdo Novo, Sabrina Torres, Carolina Perez, Andres Pybus, Oliver G. Konig, Guido Alberto Aphthovirus Phylogenetics Phylogeography Virus Fiebre Aftosa Filogenética Filogeografía Foot-and-mouth disease is a highly contagious disease affecting cloven-hoofed animals, resulting in considerable economic losses. Its causal agent is foot-and-mouth disease virus (FMDV), a picornavirus. Due to its error-prone replication and rapid evolution, the transmission and evolutionary dynamics of FMDV can be studied using genomic epidemiological approaches. To analyze FMDV evolution and identify possible transmission routes in an Argentinean region, field samples that tested positive for FMDV by PCR were obtained from 21 farms located in the Mar Chiquita district. Whole FMDV genome sequences were obtained by PCR amplification in seven fragments and sequencing using the Sanger technique. The genome sequences obtained from these samples were then analyzed using phylogenetic, phylogeographic, and evolutionary approaches. Three local transmission clusters were detected among the sampled viruses. The dataset was analyzed using Bayesian phylodynamic methods with appropriate coalescent and relaxed molecular clock models. The estimated mean viral evolutionary rate was 1.17 × 10− 2 substitutions/site/year. No significant differences in the rate of viral evolution were observed between farms with vaccinated animals and those with unvaccinated animals. The most recent common ancestor of the sampled sequences was dated to approximately one month before the first reported case in the outbreak. Virus transmission started in the south of the district and later dispersed to the west, and finally arrived in the east. Different transmission routes among the studied herds, such as non-replicating vectors and close contact contagion (i.e., aerosols), may be responsible for viral spread. Instituto de Biotecnología Fil: Lozano Calderon, Laura Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lozano Calderon, Laura Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cabanne, Gustavo Sebastián. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina Fil: Cabanne, Gustavo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marcos, Andrea. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Coordinación general de Epidemiología y Análisis de Riesgo; Argentina Fil: Galdo Novo, Sabrina. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección de Laboratorio Animal; Argentina Fil: Torres, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM); Argentina Fil: Perez, Andres. University of Minnesota. Department of Veterinary Population Medicine; Estados Unidos Fil: Pybus, Oliver G. University of Oxford. Department of Biology; Reino Unido Fil: Pybus, Oliver G. The Royal Veterinary College. Department of Pathobiology and Population Sciences; Reino Unido Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Konig, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-03T09:32:16Z 2024-07-03T09:32:16Z 2024-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18362 https://link.springer.com/article/10.1007/s00705-024-06028-0 1432-8798 https://doi.org/10.1007/s00705-024-06028-0 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Archives of Virology 169 (5) : 101 (May 2024) |
| spellingShingle | Aphthovirus Phylogenetics Phylogeography Virus Fiebre Aftosa Filogenética Filogeografía Lozano Calderon, Laura Camila Cabanne, Gustavo Sebastián Marcos, Andrea Galdo Novo, Sabrina Torres, Carolina Perez, Andres Pybus, Oliver G. Konig, Guido Alberto Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title | Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title_full | Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title_fullStr | Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title_full_unstemmed | Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title_short | Phylodynamic analysis of foot-and-mouth disease virus evolution in Mar Chiquita, Argentina |
| title_sort | phylodynamic analysis of foot and mouth disease virus evolution in mar chiquita argentina |
| topic | Aphthovirus Phylogenetics Phylogeography Virus Fiebre Aftosa Filogenética Filogeografía |
| url | http://hdl.handle.net/20.500.12123/18362 https://link.springer.com/article/10.1007/s00705-024-06028-0 https://doi.org/10.1007/s00705-024-06028-0 |
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