Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polero...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18293 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13934 https://doi.org/10.1111/ppa.13934 |
| _version_ | 1855486145786281984 |
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| author | Delfosse, Veronica Cecilia Debat, Humberto Julio Zavallo, Diego Alvarez, Liliana Gomez Talquenca, Gonzalo Bonacic Kresic, Iván Asurmendi, Sebastian Distefano, Ana Julia |
| author_browse | Alvarez, Liliana Asurmendi, Sebastian Bonacic Kresic, Iván Debat, Humberto Julio Delfosse, Veronica Cecilia Distefano, Ana Julia Gomez Talquenca, Gonzalo Zavallo, Diego |
| author_facet | Delfosse, Veronica Cecilia Debat, Humberto Julio Zavallo, Diego Alvarez, Liliana Gomez Talquenca, Gonzalo Bonacic Kresic, Iván Asurmendi, Sebastian Distefano, Ana Julia |
| author_sort | Delfosse, Veronica Cecilia |
| collection | INTA Digital |
| description | Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton. |
| format | Artículo |
| id | INTA18293 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA182932024-06-28T10:53:41Z Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus Delfosse, Veronica Cecilia Debat, Humberto Julio Zavallo, Diego Alvarez, Liliana Gomez Talquenca, Gonzalo Bonacic Kresic, Iván Asurmendi, Sebastian Distefano, Ana Julia Begomovirus Cotton Algodón Gossypium Polerovirus Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. Cotton blue disease is caused by cotton leafroll dwarf virus (CLRDV), a polerovirus transmitted by aphids. This virus is controlled by sowing cotton varieties resistant to CLRDV, although, in recent years an atypical variant (CLRDV-at), that breaks the resistance, has been identified. An outbreak of a disease occurred in cotton fields of the province of Chaco, in the north-west of Argentina, in the 2014–2015 growing season. NuOpal cotton plants (a variety resistant to CLRDV and susceptible to CLRDV-at) showed mosaic symptoms and had virus-transmitting insect vectors, such as aphids and whiteflies (Bemisia tabaci). The symptoms were common for virus infections but different from those caused by CLRDV or CLRDV-at. According to a reverse transcription-PCR analysis with polerovirus primers and sequencing, the plants contained CLRDV-at. To identify the complete virome of these plants, we performed a massive RNA-based deep sequencing assay using Illumina technology. As a result, a new begomovirus that infects cotton in Argentina was identified. Structural and functional annotation indicated that its sequences corresponded to complete DNA components A and B of a novel New World bipartite begomovirus. Genetic distance and evolutionary analyses support that the detected sequences correspond to a new virus, which we propose to name cotton mosaic virus (CoMV). In addition, we report the first begomovirus and polerovirus coinfection in cotton. Instituto de Biotecnología Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina Fil: Debat, Humberto Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez Talquenca, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Distefano, Ana Julia. Universidad Nacional de Luján; Argentina 2024-06-28T10:01:38Z 2024-06-28T10:01:38Z 2024-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18293 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13934 1365-3059 https://doi.org/10.1111/ppa.13934 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Pathology 00 : 1–11 (First published: 22 May 2024) |
| spellingShingle | Begomovirus Cotton Algodón Gossypium Polerovirus Delfosse, Veronica Cecilia Debat, Humberto Julio Zavallo, Diego Alvarez, Liliana Gomez Talquenca, Gonzalo Bonacic Kresic, Iván Asurmendi, Sebastian Distefano, Ana Julia Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title | Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title_full | Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title_fullStr | Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title_full_unstemmed | Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title_short | Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| title_sort | coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus |
| topic | Begomovirus Cotton Algodón Gossypium Polerovirus |
| url | http://hdl.handle.net/20.500.12123/18293 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13934 https://doi.org/10.1111/ppa.13934 |
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