First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria
Sarcocystis are Apicomplexan protozoa with a dixenous life cycle that includes a predator and a prey as definitive and intermediate hosts, respectively. Domestic and wild pigs are intermediate hosts of S. suihominis, with formation of sarcocysts in their muscles, while humans and non-human primates...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/18234 https://link.springer.com/article/10.1007/s00436-024-08160-x https://doi.org/10.1007/s00436-024-08160-x |
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| author | Obadiah, Happiness Igwe Wieser, Sara Nathaly Nzelu, Ifeoma Nancy Olaolu, Olushola Samuel Jagab, Hafsat Shaiabu Obishakin, Emmanuel Tumininu Omudu, Edward Agbo Atu, Bernard Ortwer Byanet, Obadiah Schnittger, Leonhard Florin-Christensen, Monica |
| author_browse | Atu, Bernard Ortwer Byanet, Obadiah Florin-Christensen, Monica Jagab, Hafsat Shaiabu Nzelu, Ifeoma Nancy Obadiah, Happiness Igwe Obishakin, Emmanuel Tumininu Olaolu, Olushola Samuel Omudu, Edward Agbo Schnittger, Leonhard Wieser, Sara Nathaly |
| author_facet | Obadiah, Happiness Igwe Wieser, Sara Nathaly Nzelu, Ifeoma Nancy Olaolu, Olushola Samuel Jagab, Hafsat Shaiabu Obishakin, Emmanuel Tumininu Omudu, Edward Agbo Atu, Bernard Ortwer Byanet, Obadiah Schnittger, Leonhard Florin-Christensen, Monica |
| author_sort | Obadiah, Happiness Igwe |
| collection | INTA Digital |
| description | Sarcocystis are Apicomplexan protozoa with a dixenous life cycle that includes a predator and a prey as definitive and intermediate hosts, respectively. Domestic and wild pigs are intermediate hosts of S. suihominis, with formation of sarcocysts in their muscles, while humans and non-human primates act as final hosts. After ingesting raw or undercooked sarcocyst-infested pork, signs of gastroenteritis including inappetence, nausea, vomiting, and diarrhea may develop in humans. Moreover, excretion of infective forms with human feces leads to dissemination of the parasite in the environment. In this study, macroscopic sarcocysts of white color, oval shape, and a diameter of approximately 3–8 mm were found in the skeletal muscle of a slaughtered domestic pig (Sus scrofa domesticus) destined for human consumption in an abattoir of Makurdi, Benue State, Nigeria. Sarcocyst DNA was used as template to PCR amplify the near-complete length of the 18S rRNA gene and a fragment of the cytochrome c oxidase subunit 1 (cox-1) gene. Amplicons were sequenced and used to construct phylogenetic trees with selected available Sarcocystis spp. sequences. In both cases, the placement of the analyzed sequences with S. suihominis was strongly supported, confirming the species identity of this macroscopic sarcocyst-forming parasite. This constitutes the first molecular identification of S. suihominis in Nigeria and the African continent. Proximity between pigs and humans, and poor sanitary conditions frequently encountered in pig farms of Nigeria might favor the dissemination of this zoonotic parasite, posing a threat to public health. |
| format | Artículo |
| id | INTA18234 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA182342024-06-24T10:39:46Z First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria Obadiah, Happiness Igwe Wieser, Sara Nathaly Nzelu, Ifeoma Nancy Olaolu, Olushola Samuel Jagab, Hafsat Shaiabu Obishakin, Emmanuel Tumininu Omudu, Edward Agbo Atu, Bernard Ortwer Byanet, Obadiah Schnittger, Leonhard Florin-Christensen, Monica Pork Phylogenetic Analysis Parasites Carne de Cerdo Análisis Filogenético Parásitos Nigeria Sarcocystis suihominis Sarcocystis are Apicomplexan protozoa with a dixenous life cycle that includes a predator and a prey as definitive and intermediate hosts, respectively. Domestic and wild pigs are intermediate hosts of S. suihominis, with formation of sarcocysts in their muscles, while humans and non-human primates act as final hosts. After ingesting raw or undercooked sarcocyst-infested pork, signs of gastroenteritis including inappetence, nausea, vomiting, and diarrhea may develop in humans. Moreover, excretion of infective forms with human feces leads to dissemination of the parasite in the environment. In this study, macroscopic sarcocysts of white color, oval shape, and a diameter of approximately 3–8 mm were found in the skeletal muscle of a slaughtered domestic pig (Sus scrofa domesticus) destined for human consumption in an abattoir of Makurdi, Benue State, Nigeria. Sarcocyst DNA was used as template to PCR amplify the near-complete length of the 18S rRNA gene and a fragment of the cytochrome c oxidase subunit 1 (cox-1) gene. Amplicons were sequenced and used to construct phylogenetic trees with selected available Sarcocystis spp. sequences. In both cases, the placement of the analyzed sequences with S. suihominis was strongly supported, confirming the species identity of this macroscopic sarcocyst-forming parasite. This constitutes the first molecular identification of S. suihominis in Nigeria and the African continent. Proximity between pigs and humans, and poor sanitary conditions frequently encountered in pig farms of Nigeria might favor the dissemination of this zoonotic parasite, posing a threat to public health. Instituto de Patobiología Fil: Obadiah, Happiness Igwe. Benue State University. Department of Biological Sciences; Nigeria Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nzelu, Ifeoma Nancy. Joseph Sarwuan Tarka University. College of Veterinary Medicine. Department of Veterinary Public Health and Preventive Medicine; Nigeria Fil: Olaolu, Olushola Samuel. National Veterinary Research Institute. Biotechnology Center; Nigeria Fil: Jagab, Hafsat Shaiabu. National Veterinary Research Institute. Biotechnology Center; Nigeria Fil: Obishakin, Emmanuel Tumininu. National Veterinary Research Institute. Biotechnology Center; Nigeria Fil: Omudu, Edward Agbo. Benue State University. Department of Biological Sciences; Nigeria Fil: Atu, Bernard Ortwer. Benue State University. Department of Biological Sciences; Nigeria Fil: Byanet, Obadiah. University of Arizona. College of Veterinary Medicine; Estados Unidos Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-06-24T10:32:51Z 2024-06-24T10:32:51Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18234 https://link.springer.com/article/10.1007/s00436-024-08160-x 1432-1955 https://doi.org/10.1007/s00436-024-08160-x eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Parasitology Research 123 : 142 (Published: 23 February 2024) |
| spellingShingle | Pork Phylogenetic Analysis Parasites Carne de Cerdo Análisis Filogenético Parásitos Nigeria Sarcocystis suihominis Obadiah, Happiness Igwe Wieser, Sara Nathaly Nzelu, Ifeoma Nancy Olaolu, Olushola Samuel Jagab, Hafsat Shaiabu Obishakin, Emmanuel Tumininu Omudu, Edward Agbo Atu, Bernard Ortwer Byanet, Obadiah Schnittger, Leonhard Florin-Christensen, Monica First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title | First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title_full | First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title_fullStr | First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title_full_unstemmed | First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title_short | First molecular detection of Sarcocystis suihominis in a domestic pig of Nigeria |
| title_sort | first molecular detection of sarcocystis suihominis in a domestic pig of nigeria |
| topic | Pork Phylogenetic Analysis Parasites Carne de Cerdo Análisis Filogenético Parásitos Nigeria Sarcocystis suihominis |
| url | http://hdl.handle.net/20.500.12123/18234 https://link.springer.com/article/10.1007/s00436-024-08160-x https://doi.org/10.1007/s00436-024-08160-x |
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