Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis
Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measur...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer Nature
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18233 https://www.nature.com/articles/s41598-024-52089-1 https://doi.org/10.1038/s41598-024-52089-1 |
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| author | Echeverria, Gustavo Zumarraga, Martin Jose Proaño‑Pérez, Freddy Barceló Blasco, Francisco Waard, Jacobus H. de |
| author_browse | Barceló Blasco, Francisco Echeverria, Gustavo Proaño‑Pérez, Freddy Waard, Jacobus H. de Zumarraga, Martin Jose |
| author_facet | Echeverria, Gustavo Zumarraga, Martin Jose Proaño‑Pérez, Freddy Barceló Blasco, Francisco Waard, Jacobus H. de |
| author_sort | Echeverria, Gustavo |
| collection | INTA Digital |
| description | Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18233 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA182332024-06-24T10:30:12Z Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis Echeverria, Gustavo Zumarraga, Martin Jose Proaño‑Pérez, Freddy Barceló Blasco, Francisco Waard, Jacobus H. de Tuberculin Bovine Tuberculosis Diagnosis Tuberculina Tuberculosis Bovina Diagnóstico Although several brands of tuberculin purified protein derivatives (PPDs) are available for diagnosing bovine tuberculosis (bTB), comparative studies to determine their diagnostic accuracy are infrequent. In Ecuador we compared two different PPD brands for bTB diagnosis using skin testing and measuring skin thickness increase. Additionally, we evaluated four PPD brands, including those used for skin testing, in the Bovine Tuberculosis Interferon Gamma Test (IFN-γ test) measuring IFN-γ induction in whole blood. The study included 17 naturally tuberculosis-infected PPD and IFN-γ test positive bovines. Both the field and laboratory results showed significant differences in classifying the 17 bovines as bTB positive or negative. We hypothesize that several factors, such as the genetic background of the cows, sensitization to environmental mycobacteria, M. bovis strains involved in the bTB infection, and the manufacturing procedures of the PPDs, could have influenced the immune reaction toward the different tuberculin PPD brands. Our study emphasizes the necessity for comparative studies aimed at determining the diagnostic accuracy of PPD brands for bTB diagnosis as well as the development of standardized methods for PPD production and potency determination. Instituto de Biotecnología Fil: Echeverria, Gustavo. Universidad Central del Ecuador. Instituto de Investigación en Zoonosis-CIZ; Ecuador Fil: Echeverria, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Echeverria, Gustavo. BioGENA. División Investigación y Desarrollo; Ecuador Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Proaño‑Pérez, Freddy. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia; Ecuador Fil: Barceló Blasco, Francisco. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador Fil: Waard, Jacobus H. de. Universidad de las Américas. Facultad de Ciencias de la Salud. One Health Research Group; Ecuador 2024-06-24T10:27:20Z 2024-06-24T10:27:20Z 2024-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18233 https://www.nature.com/articles/s41598-024-52089-1 2045-2322 https://doi.org/10.1038/s41598-024-52089-1 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Scientifc Reports 14 : 5155 (Published: 02 March 2024) |
| spellingShingle | Tuberculin Bovine Tuberculosis Diagnosis Tuberculina Tuberculosis Bovina Diagnóstico Echeverria, Gustavo Zumarraga, Martin Jose Proaño‑Pérez, Freddy Barceló Blasco, Francisco Waard, Jacobus H. de Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title | Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title_full | Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title_fullStr | Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title_full_unstemmed | Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title_short | Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis |
| title_sort | assessing the impact of various tuberculin ppd brands on bovine tuberculosis diagnosis |
| topic | Tuberculin Bovine Tuberculosis Diagnosis Tuberculina Tuberculosis Bovina Diagnóstico |
| url | http://hdl.handle.net/20.500.12123/18233 https://www.nature.com/articles/s41598-024-52089-1 https://doi.org/10.1038/s41598-024-52089-1 |
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