Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris

One isolate of Metarhizium anisopliae (Ma 72) previously selected for its virulence against rice-stem bugs, Tibraca limbativentris, was studied. Its conidial production and quality parameters in several solid substrates, as well as its reusability, were evaluated. Conidial germination percentages (%...

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Autores principales: Kruger, Raúl, Posadas, Julieta Beatriz, Angulo Lewylle, Maricel, Mini, Jorge Ignacio, Lecuona, Roberto Eduardo
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: IDOSI Publications 2024
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/18123
https://www.idosi.org/wasj/wasj32(7)14/8.pdf
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author Kruger, Raúl
Posadas, Julieta Beatriz
Angulo Lewylle, Maricel
Mini, Jorge Ignacio
Lecuona, Roberto Eduardo
author_browse Angulo Lewylle, Maricel
Kruger, Raúl
Lecuona, Roberto Eduardo
Mini, Jorge Ignacio
Posadas, Julieta Beatriz
author_facet Kruger, Raúl
Posadas, Julieta Beatriz
Angulo Lewylle, Maricel
Mini, Jorge Ignacio
Lecuona, Roberto Eduardo
author_sort Kruger, Raúl
collection INTA Digital
description One isolate of Metarhizium anisopliae (Ma 72) previously selected for its virulence against rice-stem bugs, Tibraca limbativentris, was studied. Its conidial production and quality parameters in several solid substrates, as well as its reusability, were evaluated. Conidial germination percentages (%G) in two experimental formulations - broken white rice and parboil rice - were evaluated at different storage times (0, 50 and 100 days) at 6°C and 20°C. Ma 72 was able to produce conidia in all the substrates. When used for the first time, the broken white rice presented the highest production parameters, with 4.4 x10 10 conidia/g conidial powder. Its production parameter was even higher than that of parboil rice (control). Nevertheless, when the substrates were reused, parboil rice presented the highest value. In both experimental formulations, the%G decreased from 97% to less than 87.32% and 85.11% at 6°C after 50 and 100 days of room storage, respectively. At 20°C the%G presented values below 51.42% and 16.47% after 50 and 100 days of storage, respectively. Only parboil rice could be reused successfully, but at a considerable cost. In contrast, broken white rice might be suitable as an economical substrate but would not be reused, thus probably increasing production wastes. Using broken white rice as part of a wettable powder formulation may reduce these wastes.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA181232024-06-12T11:29:27Z Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris Kruger, Raúl Posadas, Julieta Beatriz Angulo Lewylle, Maricel Mini, Jorge Ignacio Lecuona, Roberto Eduardo Arroz Oryza sativa Plagas de Plantas Control Biológico Metarhizium anisopliae Hongos Entomopatogenos Rice Plant Pests Biological Control Entomogenous Fungi Tibraca limbativentris One isolate of Metarhizium anisopliae (Ma 72) previously selected for its virulence against rice-stem bugs, Tibraca limbativentris, was studied. Its conidial production and quality parameters in several solid substrates, as well as its reusability, were evaluated. Conidial germination percentages (%G) in two experimental formulations - broken white rice and parboil rice - were evaluated at different storage times (0, 50 and 100 days) at 6°C and 20°C. Ma 72 was able to produce conidia in all the substrates. When used for the first time, the broken white rice presented the highest production parameters, with 4.4 x10 10 conidia/g conidial powder. Its production parameter was even higher than that of parboil rice (control). Nevertheless, when the substrates were reused, parboil rice presented the highest value. In both experimental formulations, the%G decreased from 97% to less than 87.32% and 85.11% at 6°C after 50 and 100 days of room storage, respectively. At 20°C the%G presented values below 51.42% and 16.47% after 50 and 100 days of storage, respectively. Only parboil rice could be reused successfully, but at a considerable cost. In contrast, broken white rice might be suitable as an economical substrate but would not be reused, thus probably increasing production wastes. Using broken white rice as part of a wettable powder formulation may reduce these wastes. Instituto de Microbiología y Zoología Agrícola Fil: Kruger, Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Posadas, Julieta Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Angulo Lewylle, Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Mini, Jorge I. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Lecuona, Roberto Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2024-06-12T11:24:32Z 2024-06-12T11:24:32Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18123 https://www.idosi.org/wasj/wasj32(7)14/8.pdf 1818-4952 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf IDOSI Publications World Applied Sciences Journal 32 (7) : 1242-1251. (June 2014)
spellingShingle Arroz
Oryza sativa
Plagas de Plantas
Control Biológico
Metarhizium anisopliae
Hongos Entomopatogenos
Rice
Plant Pests
Biological Control
Entomogenous Fungi
Tibraca limbativentris
Kruger, Raúl
Posadas, Julieta Beatriz
Angulo Lewylle, Maricel
Mini, Jorge Ignacio
Lecuona, Roberto Eduardo
Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title_full Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title_fullStr Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title_full_unstemmed Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title_short Solid Substrate Production and Formulation of an Isolate of Metarhizium anisopliae for Biological Control of Stem Bug Tibraca limbativentris
title_sort solid substrate production and formulation of an isolate of metarhizium anisopliae for biological control of stem bug tibraca limbativentris
topic Arroz
Oryza sativa
Plagas de Plantas
Control Biológico
Metarhizium anisopliae
Hongos Entomopatogenos
Rice
Plant Pests
Biological Control
Entomogenous Fungi
Tibraca limbativentris
url http://hdl.handle.net/20.500.12123/18123
https://www.idosi.org/wasj/wasj32(7)14/8.pdf
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