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In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection

The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bo...

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Main Authors: Ynsaurralde Rivolta, Amanda Eugenia, Gambini, Andrés, Alberio, Virgilia, Savy, Virginia, Ratner, Laura Daniela, Guberman, Alejandra, Vazquez Echegaray, Camila, Gismondi, Maria Ines, Curra, Anabella Paola, Bevacqua, Romina Jimena, Salamone, Daniel Felipe
Format: Artículo
Language:Inglés
Published: Wiley 2024
Subjects:
Embriones Animales
Ganado Bovino
Experimentación in Vitro
Genética
Animal Embryos
Cattle
In Vitro Experimentation
Genetics
Blastocysts
Online Access:http://hdl.handle.net/20.500.12123/18064
https://onlinelibrary.wiley.com/doi/10.1111/rda.14627
https://doi.org/10.1111/rda.14627
Summary:The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies: (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.

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