Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina
The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopic observati...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18058 https://link.springer.com/article/10.1007/s00436-023-08036-6 https://doi.org/10.1007/s00436-023-08036-6 |
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| author | Bentancourt Rossoli, Judith Victoria Moré, Gastón Andrés Soto Cabrera, Agustina Moore, Prando Dadin Morrell, Eleonora Lidia Pedrana, Julieta Scioli, Maria Valeria Campero, Lucia Maria Basso, Walter Hecker, Yanina Scioscia, Nathalia Paula |
| author_browse | Basso, Walter Bentancourt Rossoli, Judith Victoria Campero, Lucia Maria Hecker, Yanina Moore, Prando Dadin Morrell, Eleonora Lidia Moré, Gastón Andrés Pedrana, Julieta Scioli, Maria Valeria Scioscia, Nathalia Paula Soto Cabrera, Agustina |
| author_facet | Bentancourt Rossoli, Judith Victoria Moré, Gastón Andrés Soto Cabrera, Agustina Moore, Prando Dadin Morrell, Eleonora Lidia Pedrana, Julieta Scioli, Maria Valeria Campero, Lucia Maria Basso, Walter Hecker, Yanina Scioscia, Nathalia Paula |
| author_sort | Bentancourt Rossoli, Judith Victoria |
| collection | INTA Digital |
| description | The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopic observation, respectively. PCR-sequencing targeting the 18S rRNA, cox1, and ITS1 regions was performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6–100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae, and Necromys lasiurus. Eight sequences showed 99.5–99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3–96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical to each other and showed a high similarity with S. strixi (99.2–99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not lead to an accurate species identification. Possible reasons are the detection of new species or the limited molecular information available from previously described Sarcocystis spp. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as definitive hosts. This study represents the first molecular identification of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18058 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA180582024-06-07T10:24:29Z Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina Bentancourt Rossoli, Judith Victoria Moré, Gastón Andrés Soto Cabrera, Agustina Moore, Prando Dadin Morrell, Eleonora Lidia Pedrana, Julieta Scioli, Maria Valeria Campero, Lucia Maria Basso, Walter Hecker, Yanina Scioscia, Nathalia Paula Sarcocistosis Sarcocystis Roedores Histología Argentina Rodents Histology PCR Sarcocystosis The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopic observation, respectively. PCR-sequencing targeting the 18S rRNA, cox1, and ITS1 regions was performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6–100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae, and Necromys lasiurus. Eight sequences showed 99.5–99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3–96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical to each other and showed a high similarity with S. strixi (99.2–99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not lead to an accurate species identification. Possible reasons are the detection of new species or the limited molecular information available from previously described Sarcocystis spp. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as definitive hosts. This study represents the first molecular identification of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America. EEA Balcarce Fil: Bentancourt Rossoli, Judith Victoria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moré, Gastón Andrés. University of Bern. Vetsuisse Faculty. Institute of Parasitology. Department of Infectious Diseases and Pathobiology; Suiza Fil: Soto Cabrera, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Pedrana, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scioli, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Basso, Walter. University of Bern. Vetsuisse Faculty. Institute of Parasitology, Department of Infectious Diseases and Pathobiology; Suiza Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Scioscia, Nathalia Paula. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Sanidad Producción y Ambiente; Argentina 2024-06-07T10:07:39Z 2024-06-07T10:07:39Z 2024 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18058 https://link.springer.com/article/10.1007/s00436-023-08036-6 0932-0113 (Print) 1432-1955 (Online) https://doi.org/10.1007/s00436-023-08036-6 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Parasitology Research 123 : article number 31 (2024) |
| spellingShingle | Sarcocistosis Sarcocystis Roedores Histología Argentina Rodents Histology PCR Sarcocystosis Bentancourt Rossoli, Judith Victoria Moré, Gastón Andrés Soto Cabrera, Agustina Moore, Prando Dadin Morrell, Eleonora Lidia Pedrana, Julieta Scioli, Maria Valeria Campero, Lucia Maria Basso, Walter Hecker, Yanina Scioscia, Nathalia Paula Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title | Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title_full | Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title_fullStr | Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title_full_unstemmed | Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title_short | Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina |
| title_sort | identification of sarcocystis spp in synanthropic muridae and wild cricetidae rodents from argentina |
| topic | Sarcocistosis Sarcocystis Roedores Histología Argentina Rodents Histology PCR Sarcocystosis |
| url | http://hdl.handle.net/20.500.12123/18058 https://link.springer.com/article/10.1007/s00436-023-08036-6 https://doi.org/10.1007/s00436-023-08036-6 |
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