Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations
Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molec...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Cambridge University Press
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/17935 https://www.cambridge.org/core/journals/plant-genetic-resources/article/abs/genetic-characterization-of-sunflower-breeding-resources-from-argentina-assessing-diversity-in-key-openpollinated-and-composite-populations/B6792D5DA3E0E2D689BF34B84449224A https://doi.org/10.1017/S1479262113000075 |
| _version_ | 1855486071814488064 |
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| author | Moreno, Maria Valeria Nishinakamasu, Veronica Loray, M.A. Alvarez, Daniel Gieco, Jorge Omar Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_browse | Alvarez, Daniel Gieco, Jorge Omar Heinz, Ruth Amelia Hopp, Horacio Esteban Lia, Veronica Viviana Loray, M.A. Moreno, Maria Valeria Nishinakamasu, Veronica Paniego, Norma Beatriz |
| author_facet | Moreno, Maria Valeria Nishinakamasu, Veronica Loray, M.A. Alvarez, Daniel Gieco, Jorge Omar Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_sort | Moreno, Maria Valeria |
| collection | INTA Digital |
| description | Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnologı´a Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were
recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates of FST revealed very high levels of differentiation among accessions (FST ¼ 0.413, P , 0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections. |
| format | Artículo |
| id | INTA17935 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | INTA179352024-05-29T10:25:16Z Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations Moreno, Maria Valeria Nishinakamasu, Veronica Loray, M.A. Alvarez, Daniel Gieco, Jorge Omar Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Cultivated Land Sunflowers Germplasm Genetic Diversity Plant Breeding Genetic Variation Genetic Markers Tierras Cultivadas Girasol Germoplasma Diversidad Genética Fitomejoramiento Variación Genética Marcadores Genéticos Argentina Molecular Markers Agromorphological Descriptors Marcadores Moleculares Descriptores Agromorfológicos Open-pollinated (OPs) and composite populations (CPs) represent a valuable resource for sunflower breeding programmes. However, little is known about the levels and distribution of genetic variation within each of these populations. In this study, quantitative and qualitative traits along with molecular markers were used to evaluate 14 populations from the Instituto Nacional de Tecnologı´a Agropecuaria (INTA) sunflower germplasm collection. These populations were chosen to represent historically important accessions that still play a central role within the INTA sunflower breeding programme due to their extensive variation in diverse agronomically important traits. Nine quantitative and eight qualitative agro-morphological descriptors were recorded and compared with those of a larger set of accessions representative of the phenotypic diversity of the sunflower collection. Molecular characterization was conducted on a total of 311 individuals using 16 microsatellite markers. Overall, the average gene diversity was 0.56 and the average number of alleles per locus was 6.25. No statistically significant differences in genetic diversity were detected between the OPs and CPs. Global estimates of FST revealed very high levels of differentiation among accessions (FST ¼ 0.413, P , 0.05). Population structure analyses were consistent with the observed levels of differentiation and identified two major groups. The results of this work show that high global diversity is preserved within the accessions analysed here. Additionally, this study provides a set of reliable and discriminant markers for the cost-effective molecular characterization of sunflower accessions, along with the guidelines for the delineation of sampling strategies for OPs and CPs, thus aiding the efficient management and exploitation of sunflower germplasm collections. EEA Manfredi Fil: Moreno, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Nishinakamasu, Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Loray, M. A. Instituto Nacional de Semillas (INASE), Buenos Aires; Argentina Fil: Alvarez, Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Gieco, Jorge Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Grupo de Biotecnología en Cultivo. Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2024-05-29T10:09:44Z 2024-05-29T10:09:44Z 2013-03-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17935 https://www.cambridge.org/core/journals/plant-genetic-resources/article/abs/genetic-characterization-of-sunflower-breeding-resources-from-argentina-assessing-diversity-in-key-openpollinated-and-composite-populations/B6792D5DA3E0E2D689BF34B84449224A 1479-2621 (Print) 1479-263X (Online) https://doi.org/10.1017/S1479262113000075 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Cambridge University Press Plant Genetic Resources 11 (3) : 238-249. (2013) |
| spellingShingle | Cultivated Land Sunflowers Germplasm Genetic Diversity Plant Breeding Genetic Variation Genetic Markers Tierras Cultivadas Girasol Germoplasma Diversidad Genética Fitomejoramiento Variación Genética Marcadores Genéticos Argentina Molecular Markers Agromorphological Descriptors Marcadores Moleculares Descriptores Agromorfológicos Moreno, Maria Valeria Nishinakamasu, Veronica Loray, M.A. Alvarez, Daniel Gieco, Jorge Omar Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title | Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title_full | Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title_fullStr | Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title_full_unstemmed | Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title_short | Genetic characterization of sunflower breeding resources from Argentina: assessing diversity in key open-pollinated and composite populations |
| title_sort | genetic characterization of sunflower breeding resources from argentina assessing diversity in key open pollinated and composite populations |
| topic | Cultivated Land Sunflowers Germplasm Genetic Diversity Plant Breeding Genetic Variation Genetic Markers Tierras Cultivadas Girasol Germoplasma Diversidad Genética Fitomejoramiento Variación Genética Marcadores Genéticos Argentina Molecular Markers Agromorphological Descriptors Marcadores Moleculares Descriptores Agromorfológicos |
| url | http://hdl.handle.net/20.500.12123/17935 https://www.cambridge.org/core/journals/plant-genetic-resources/article/abs/genetic-characterization-of-sunflower-breeding-resources-from-argentina-assessing-diversity-in-key-openpollinated-and-composite-populations/B6792D5DA3E0E2D689BF34B84449224A https://doi.org/10.1017/S1479262113000075 |
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