Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance
The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding prot...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1778 https://link.springer.com/article/10.1007%2Fs00299-016-1956-4 |
| _version_ | 1855034853261574144 |
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| author | Genero, Melisa Gismondi, Mauro Monti, Laura Gabilondo, Julieta Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Bustamante, Claudia Anabel |
| author_browse | Andreo, Carlos Santiago Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Gabilondo, Julieta Genero, Melisa Gismondi, Mauro Lara, María Valeria Monti, Laura |
| author_facet | Genero, Melisa Gismondi, Mauro Monti, Laura Gabilondo, Julieta Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Bustamante, Claudia Anabel |
| author_sort | Genero, Melisa |
| collection | INTA Digital |
| description | The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach cultivars. The resistant (‘Springlady’) and susceptible (‘Flordaking’) cultivars displayed differences in the level of expression of some of the selected genes during fruit softening and in woolly versus non-woolly fruits. From these genes, the level of expression of PpXyl, which encodes for a putative β-xylosidase, was the one that presented the highest correlation (negative) with the susceptibility to woolliness. PpXyl expression was also analysed in a cultivar (‘Rojo 2’) with intermediate susceptibility to woolliness, reinforcing the conclusion about the correlation of PpXyl expression to the presence of woolliness symptom. Moreover, the level of expression of PpXyl correlated to protein level detected by Western blot. Analyses of the promoter region of the PpXyl gene (1637 bp) isolated from the three cultivars showed no differences suggesting that cis-elements from other regions of the genome and/or trans elements could be responsible of the differential PpXyl expression patterns. Overall, the results obtained indicate that PpXyl may act as a good indicator of woolliness tolerance and that the regulation of expression of this gene in different cultivars does not depend on sequences upstream the coding sequence. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1778 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA17782019-06-06T17:09:07Z Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance Genero, Melisa Gismondi, Mauro Monti, Laura Gabilondo, Julieta Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Bustamante, Claudia Anabel Durazno Peaches Prunus persica Almacenamiento en Frío Cold Storage Metabolismo Metabolism Fisiología ´Postcosecha Postharvest Physiology The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach cultivars. The resistant (‘Springlady’) and susceptible (‘Flordaking’) cultivars displayed differences in the level of expression of some of the selected genes during fruit softening and in woolly versus non-woolly fruits. From these genes, the level of expression of PpXyl, which encodes for a putative β-xylosidase, was the one that presented the highest correlation (negative) with the susceptibility to woolliness. PpXyl expression was also analysed in a cultivar (‘Rojo 2’) with intermediate susceptibility to woolliness, reinforcing the conclusion about the correlation of PpXyl expression to the presence of woolliness symptom. Moreover, the level of expression of PpXyl correlated to protein level detected by Western blot. Analyses of the promoter region of the PpXyl gene (1637 bp) isolated from the three cultivars showed no differences suggesting that cis-elements from other regions of the genome and/or trans elements could be responsible of the differential PpXyl expression patterns. Overall, the results obtained indicate that PpXyl may act as a good indicator of woolliness tolerance and that the regulation of expression of this gene in different cultivars does not depend on sequences upstream the coding sequence. EEA San Pedro Fil: Genero, Marisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro San Pedro; Argentina Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Lara, María V.Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Drincovich, María Fabiana .Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Bustamante, Claudia A.Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina 2017-12-06T13:49:30Z 2017-12-06T13:49:30Z 2016-02-23 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1778 https://link.springer.com/article/10.1007%2Fs00299-016-1956-4 1432-203X eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant cell reports 35 (6) : 1235–1246. (June 2016) |
| spellingShingle | Durazno Peaches Prunus persica Almacenamiento en Frío Cold Storage Metabolismo Metabolism Fisiología ´Postcosecha Postharvest Physiology Genero, Melisa Gismondi, Mauro Monti, Laura Gabilondo, Julieta Budde, Claudio Olaf Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Bustamante, Claudia Anabel Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title | Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title_full | Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title_fullStr | Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title_full_unstemmed | Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title_short | Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance |
| title_sort | cell wall related genes studies on peach cultivars with differential susceptibility to woolliness looking for candidates as indicators of chilling tolerance |
| topic | Durazno Peaches Prunus persica Almacenamiento en Frío Cold Storage Metabolismo Metabolism Fisiología ´Postcosecha Postharvest Physiology |
| url | http://hdl.handle.net/20.500.12123/1778 https://link.springer.com/article/10.1007%2Fs00299-016-1956-4 |
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