Exploring the cellulolytic activity of environmental mycobacteria
Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/17730 https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428 https://doi.org/10.1016/j.tube.2024.102516 |
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| author | Mon, Maria Laura Romano, Nelson Gaston Farace, Pablo Daniel Tortone, Claudia Andrea Oriani, Delia Susana Picariello, Gianluca Zumarraga, Martin Jose Gioffre, Andrea Talia, Paola Mónica |
| author_browse | Farace, Pablo Daniel Gioffre, Andrea Mon, Maria Laura Oriani, Delia Susana Picariello, Gianluca Romano, Nelson Gaston Talia, Paola Mónica Tortone, Claudia Andrea Zumarraga, Martin Jose |
| author_facet | Mon, Maria Laura Romano, Nelson Gaston Farace, Pablo Daniel Tortone, Claudia Andrea Oriani, Delia Susana Picariello, Gianluca Zumarraga, Martin Jose Gioffre, Andrea Talia, Paola Mónica |
| author_sort | Mon, Maria Laura |
| collection | INTA Digital |
| description | Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments. |
| format | Artículo |
| id | INTA17730 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA177302024-05-14T18:09:31Z Exploring the cellulolytic activity of environmental mycobacteria Mon, Maria Laura Romano, Nelson Gaston Farace, Pablo Daniel Tortone, Claudia Andrea Oriani, Delia Susana Picariello, Gianluca Zumarraga, Martin Jose Gioffre, Andrea Talia, Paola Mónica Cellulase Cellulolytic Microorganisms Celulasa Microorganismos Celulolíticos Mycolicibacterium septicum Micobacterias Ambientales Endoglucanasa Activa Environmental Mycobacteria Active Endoglucanase Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments. Instituto de Biotecnología Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Romano, Nelson Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina Fil: Picariello, Gianluca. Consiglio Nazionale Delle Ricerche. Istituto di Scienze Dell’Alimentazione; Italia Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-05-14T17:55:15Z 2024-05-14T17:55:15Z 2024-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17730 https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428 1472-9792 https://doi.org/10.1016/j.tube.2024.102516 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambiental info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud info:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genético info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado info:eu-repograntAgreement/INTA/2019-PT-E7-I159-001, Info e innovación p/ VA, agroind. y bioenergía info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Tuberculosis 147 : 102516 (July 2024) |
| spellingShingle | Cellulase Cellulolytic Microorganisms Celulasa Microorganismos Celulolíticos Mycolicibacterium septicum Micobacterias Ambientales Endoglucanasa Activa Environmental Mycobacteria Active Endoglucanase Mon, Maria Laura Romano, Nelson Gaston Farace, Pablo Daniel Tortone, Claudia Andrea Oriani, Delia Susana Picariello, Gianluca Zumarraga, Martin Jose Gioffre, Andrea Talia, Paola Mónica Exploring the cellulolytic activity of environmental mycobacteria |
| title | Exploring the cellulolytic activity of environmental mycobacteria |
| title_full | Exploring the cellulolytic activity of environmental mycobacteria |
| title_fullStr | Exploring the cellulolytic activity of environmental mycobacteria |
| title_full_unstemmed | Exploring the cellulolytic activity of environmental mycobacteria |
| title_short | Exploring the cellulolytic activity of environmental mycobacteria |
| title_sort | exploring the cellulolytic activity of environmental mycobacteria |
| topic | Cellulase Cellulolytic Microorganisms Celulasa Microorganismos Celulolíticos Mycolicibacterium septicum Micobacterias Ambientales Endoglucanasa Activa Environmental Mycobacteria Active Endoglucanase |
| url | http://hdl.handle.net/20.500.12123/17730 https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428 https://doi.org/10.1016/j.tube.2024.102516 |
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