The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil

There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n =...

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Autores principales: Garcia, Florencia, Colombatto, Darío, Brunetti, Maria Alejandra, Martinez, Maria Jose, Moreno, Maria Valeria, Scorcione Turcato, Maria Carolina, Lucini, Enrique Iván, Frossasco, Georgina, Martinez Ferrer, Jorge
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: MDPI 2024
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Acceso en línea:http://hdl.handle.net/20.500.12123/17681
https://www.mdpi.com/2076-2615/10/5/786
https://doi.org/10.3390/ani10050786
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author Garcia, Florencia
Colombatto, Darío
Brunetti, Maria Alejandra
Martinez, Maria Jose
Moreno, Maria Valeria
Scorcione Turcato, Maria Carolina
Lucini, Enrique Iván
Frossasco, Georgina
Martinez Ferrer, Jorge
author_browse Brunetti, Maria Alejandra
Colombatto, Darío
Frossasco, Georgina
Garcia, Florencia
Lucini, Enrique Iván
Martinez Ferrer, Jorge
Martinez, Maria Jose
Moreno, Maria Valeria
Scorcione Turcato, Maria Carolina
author_facet Garcia, Florencia
Colombatto, Darío
Brunetti, Maria Alejandra
Martinez, Maria Jose
Moreno, Maria Valeria
Scorcione Turcato, Maria Carolina
Lucini, Enrique Iván
Frossasco, Georgina
Martinez Ferrer, Jorge
author_sort Garcia, Florencia
collection INTA Digital
description There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n = 5) and additive (n = 6, increasing doses: 0, 0.3, 3, 30, and 300 µL/L of essential oils—EO—of Lippia turbinata or Tagetes minuta, and monensin at 1.87 mg/L). The two EO utilized were selected because they differ markedly in their chemical composition, especially in the proportion of oxygenated compounds. For both EO, the interaction between the substrate and additive was significant for all variables; however, the interaction behaved differently for the two EO. Within each substrate, the response was dose-dependent, without effects at a low level of EO and a negative outcome at the highest dose. The intermediate dose (30 µL/L) inhibited methane with a slight reduction on substrate digestibility, with L. turbinata being more effective than T. minuta. It is concluded that the effectiveness of the EO to reduce methane production depends on interactions between the substrate that is fermented and the additive dose that generates different characteristics within the incubation medium (e.g., pH); and thus, the chemical nature of the compounds of the EO modulates the magnitude of this response.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA176812024-05-09T11:03:04Z The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil Garcia, Florencia Colombatto, Darío Brunetti, Maria Alejandra Martinez, Maria Jose Moreno, Maria Valeria Scorcione Turcato, Maria Carolina Lucini, Enrique Iván Frossasco, Georgina Martinez Ferrer, Jorge Greenhouse Gases Rumen Digestion Bioactive Compounds Chemical Composition Gases de Efecto Invernadero Digestión Ruminal Compuestos Bioactivos Composición Química Plant Secondary Metabolites Metabolitos Secundarios de las Plantas There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n = 5) and additive (n = 6, increasing doses: 0, 0.3, 3, 30, and 300 µL/L of essential oils—EO—of Lippia turbinata or Tagetes minuta, and monensin at 1.87 mg/L). The two EO utilized were selected because they differ markedly in their chemical composition, especially in the proportion of oxygenated compounds. For both EO, the interaction between the substrate and additive was significant for all variables; however, the interaction behaved differently for the two EO. Within each substrate, the response was dose-dependent, without effects at a low level of EO and a negative outcome at the highest dose. The intermediate dose (30 µL/L) inhibited methane with a slight reduction on substrate digestibility, with L. turbinata being more effective than T. minuta. It is concluded that the effectiveness of the EO to reduce methane production depends on interactions between the substrate that is fermented and the additive dose that generates different characteristics within the incubation medium (e.g., pH); and thus, the chemical nature of the compounds of the EO modulates the magnitude of this response. EEA Manfredi Fil: Garcia, Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Colombatto, Darío. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Fil: Brunetti, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Martinez, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Moreno, Maria Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Scorcione Turcato, M.C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lucini, Enrique Iván. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Frossasco, Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Martinez Ferrer, Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina 2024-05-09T10:45:21Z 2024-05-09T10:45:21Z 2020-05-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17681 https://www.mdpi.com/2076-2615/10/5/786 2076-2615 https://doi.org/10.3390/ani10050786 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Animals 10 (5) : 786. (May 2020)
spellingShingle Greenhouse Gases
Rumen Digestion
Bioactive Compounds
Chemical Composition
Gases de Efecto Invernadero
Digestión Ruminal
Compuestos Bioactivos
Composición Química
Plant Secondary Metabolites
Metabolitos Secundarios de las Plantas
Garcia, Florencia
Colombatto, Darío
Brunetti, Maria Alejandra
Martinez, Maria Jose
Moreno, Maria Valeria
Scorcione Turcato, Maria Carolina
Lucini, Enrique Iván
Frossasco, Georgina
Martinez Ferrer, Jorge
The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title_full The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title_fullStr The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title_full_unstemmed The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title_short The Reduction of Methane Production in the In Vitro Ruminal Fermentation of Different Substrates is Linked with the Chemical Composition of the Essential Oil
title_sort reduction of methane production in the in vitro ruminal fermentation of different substrates is linked with the chemical composition of the essential oil
topic Greenhouse Gases
Rumen Digestion
Bioactive Compounds
Chemical Composition
Gases de Efecto Invernadero
Digestión Ruminal
Compuestos Bioactivos
Composición Química
Plant Secondary Metabolites
Metabolitos Secundarios de las Plantas
url http://hdl.handle.net/20.500.12123/17681
https://www.mdpi.com/2076-2615/10/5/786
https://doi.org/10.3390/ani10050786
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