3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets
Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollu...
| Main Authors: | , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/17680 https://www.sciencedirect.com/science/article/pii/S240584402201026X https://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X https://doi.org/10.1016/j.heliyon.2022.e09738 |
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| author | Garcia, Florencia Muñoz, Camila Martinez Ferrer, Jorge Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Immig, Irmgard Kindermann, Maik Walker, Nicola Ungerfeld, Emilio M. |
| author_browse | Garcia, Florencia Immig, Irmgard Kindermann, Maik Martinez Ferrer, Jorge Martínez, Emilio D. Muñoz, Camila Saldivia, Marcelo Ungerfeld, Emilio M. Urrutia, Natalie L. Walker, Nicola |
| author_facet | Garcia, Florencia Muñoz, Camila Martinez Ferrer, Jorge Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Immig, Irmgard Kindermann, Maik Walker, Nicola Ungerfeld, Emilio M. |
| author_sort | Garcia, Florencia |
| collection | INTA Digital |
| description | Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources. |
| format | info:ar-repo/semantics/artículo |
| id | INTA17680 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA176802024-05-09T10:42:36Z 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets Garcia, Florencia Muñoz, Camila Martinez Ferrer, Jorge Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Immig, Irmgard Kindermann, Maik Walker, Nicola Ungerfeld, Emilio M. Producción Lechera Dietas Restrictas en Proteínas Metano Efecto Invernadero Milk Production Protein Restricted Diets Methane Greenhouse Effect Dairy Cows Vacas Lecheras 3-nitrooxipropanol Methane is a potent but short-lived greenhouse gas targeted for short-term amelioration of climate change, with enteric methane emitted by ruminants being the most important anthropogenic source of methane. Ruminant production also releases nitrogen to the environment, resulting in groundwater pollution and emissions of greenhouse gas nitrous oxide. We hypothesized that inhibiting rumen methanogenesis in dairy cows with chemical inhibitor 3-nitrooxypropanol (3-NOP) would redirect metabolic hydrogen towards synthesis of microbial amino acids. Our objective was to investigate the effects of 3-NOP on methane emissions, rumen fermentation and nitrogen metabolism of dairy cows fed true protein or urea as nitrogen sources. Eight ruminally-cannulated cows were fed a plant protein or a urea-containing diet during a Control experimental period followed by a methanogenesis inhibition period with 3-NOP supplementation. All diets were unintentionally deficient in nitrogen, and diets supplemented with 3-NOP had higher fiber than diets fed in the Control period. Higher dietary f iber content in the 3-NOP period would be expected to cause higher methane emissions; however, methane emissions adjusted by dry matter and digested organic matter intake were 54% lower with 3-NOP supplementation. Also, despite of the more fibrous diet, 3-NOP shifted rumen fermentation from acetate to propionate. The post-feeding rumen ammonium peak was substantially lower in the 3-NOP period, although that did not translate into greater rumen microbial protein production nor lesser nitrogen excretion in urine. Presumably, because all diets resulted in low rumen ammonium, and intake of digestible organic matter was lower in the 3-NOP period compared to the Control period, the synthesis of microbial amino acids was limited by nitrogen and energy, precluding the evaluation of our hypothesis. Supplementation with 3-NOP was highly effective at decreasing methane emissions with a lower quality diet, both with true protein and urea as nitrogen sources. EEA Manfredi Fil: Garcia, Florencia. Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias; Argentina Fil: Muñoz, Camila. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile Fil: Martinez Ferrer, Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Urrutia, Natalie L. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Remehue; Chile Fil: Martínez, Emilio D. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile Fil: Saldivia, Marcelo. Universidad Austral de Chile. Facultad de Ciencias Veterinarias; Chile Fil: Immig, Irmgard. DSM Nutritional Products. Animal Nutrition and Health; Suiza Fil: Kindermann, Maik. DSM Nutritional Products. Animal Nutrition and Health; Suiza Fil: Walker, Nicola. DSM Nutritional Products. Animal Nutrition and Health; Suiza Fil. Ungerfeld, Emilio M. Instituto de Investigaciones Agropecuarias. Centro Regional de Investigacion Carillanca; Chile 2024-05-09T10:31:21Z 2024-05-09T10:31:21Z 2022-06-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17680 https://www.sciencedirect.com/science/article/pii/S240584402201026X https://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X 2405-8440 https://doi.org/10.1016/j.heliyon.2022.e09738 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 8 : e09738. (2022) |
| spellingShingle | Producción Lechera Dietas Restrictas en Proteínas Metano Efecto Invernadero Milk Production Protein Restricted Diets Methane Greenhouse Effect Dairy Cows Vacas Lecheras 3-nitrooxipropanol Garcia, Florencia Muñoz, Camila Martinez Ferrer, Jorge Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Immig, Irmgard Kindermann, Maik Walker, Nicola Ungerfeld, Emilio M. 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title | 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title_full | 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title_fullStr | 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title_full_unstemmed | 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title_short | 3-Nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein- or urea-containing diets |
| title_sort | 3 nitrooxypropanol substantially decreased enteric methane emissions of dairy cows fed true protein or urea containing diets |
| topic | Producción Lechera Dietas Restrictas en Proteínas Metano Efecto Invernadero Milk Production Protein Restricted Diets Methane Greenhouse Effect Dairy Cows Vacas Lecheras 3-nitrooxipropanol |
| url | http://hdl.handle.net/20.500.12123/17680 https://www.sciencedirect.com/science/article/pii/S240584402201026X https://www.cell.com/heliyon/fulltext/S2405-8440(22)01026-X https://doi.org/10.1016/j.heliyon.2022.e09738 |
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