The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion
Glycosylphosphatidylinositol-anchored proteins are abundant on the surface of pathogenic protozoans and might play an important role for parasite survival. In the present work, the relevance of GPI-anchored proteins for erythrocyte invasion of the cattle hemoparasite Babesia bovis was studied. We sh...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1755 https://www.sciencedirect.com/science/article/pii/S1877959X1400034X https://doi.org/10.1016/j.ttbdis.2013.12.011 |
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| author | Rodriguez, Anabel Elisa Florin-Christensen, Mónica Flores, Daniela Echaide, Ignacio Eduardo Suarez, Carlos Esteban Schnittger, Leonhard |
| author_browse | Echaide, Ignacio Eduardo Flores, Daniela Florin-Christensen, Mónica Rodriguez, Anabel Elisa Schnittger, Leonhard Suarez, Carlos Esteban |
| author_facet | Rodriguez, Anabel Elisa Florin-Christensen, Mónica Flores, Daniela Echaide, Ignacio Eduardo Suarez, Carlos Esteban Schnittger, Leonhard |
| author_sort | Rodriguez, Anabel Elisa |
| collection | INTA Digital |
| description | Glycosylphosphatidylinositol-anchored proteins are abundant on the surface of pathogenic protozoans and might play an important role for parasite survival. In the present work, the relevance of GPI-anchored proteins for erythrocyte invasion of the cattle hemoparasite Babesia bovis was studied. We show that cleavage of GPI-anchored antigens from the merozoite parasite stage by phosphatidylinositol-specific phospholipase C abolished invasion of erythrocytes demonstrating the importance of this class of molecules for parasite propagation. In addition, the repertoire of GPI-anchored proteins of B. bovis was predicted with high fidelity by searching its genome with available web-based bioinformatic tools. Altogether 17 GPI-anchored proteins were identified, 5 of which represent the already characterized variable merozoite surface antigens (VMSAs). Fifteen of the identified GPI-anchored proteins contain 2–26 amino acid repeats indicating that they are likely involved in functions of recognition, adhesion, or transport. Repeats were found to contain an increased frequency of proline, indicative of unstructured regions; and were estimated to be 3.21 times more hydrophilic than non-repeat regions. This suggests that they might represent eminent antibody epitopes. The majority of the putative GPI-anchored antigens reported in this work have so far remained unnoticed, though they may represent potential candidates for inclusion in a subunit vaccine. |
| format | Artículo |
| id | INTA1755 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA17552019-01-24T17:53:14Z The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion Rodriguez, Anabel Elisa Florin-Christensen, Mónica Flores, Daniela Echaide, Ignacio Eduardo Suarez, Carlos Esteban Schnittger, Leonhard Babesia Bovis Eritrocitos Erythrocytes Babesiosis Glycosylphosphatidylinositol Glycosylphosphatidylinositol-anchored proteins are abundant on the surface of pathogenic protozoans and might play an important role for parasite survival. In the present work, the relevance of GPI-anchored proteins for erythrocyte invasion of the cattle hemoparasite Babesia bovis was studied. We show that cleavage of GPI-anchored antigens from the merozoite parasite stage by phosphatidylinositol-specific phospholipase C abolished invasion of erythrocytes demonstrating the importance of this class of molecules for parasite propagation. In addition, the repertoire of GPI-anchored proteins of B. bovis was predicted with high fidelity by searching its genome with available web-based bioinformatic tools. Altogether 17 GPI-anchored proteins were identified, 5 of which represent the already characterized variable merozoite surface antigens (VMSAs). Fifteen of the identified GPI-anchored proteins contain 2–26 amino acid repeats indicating that they are likely involved in functions of recognition, adhesion, or transport. Repeats were found to contain an increased frequency of proline, indicative of unstructured regions; and were estimated to be 3.21 times more hydrophilic than non-repeat regions. This suggests that they might represent eminent antibody epitopes. The majority of the putative GPI-anchored antigens reported in this work have so far remained unnoticed, though they may represent potential candidates for inclusion in a subunit vaccine. Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Flores, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. ANPCyT; Argentina Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Suarez, Carlos Esteban. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos Fil: Schnittger, Leonhard. . Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-11-13T13:38:21Z 2017-11-13T13:38:21Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1755 https://www.sciencedirect.com/science/article/pii/S1877959X1400034X 1877-959X (Print) 1877-9603 (Online) https://doi.org/10.1016/j.ttbdis.2013.12.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Ticks and tick-borne diseases 5 (3) : 343-348. (April 2014) |
| spellingShingle | Babesia Bovis Eritrocitos Erythrocytes Babesiosis Glycosylphosphatidylinositol Rodriguez, Anabel Elisa Florin-Christensen, Mónica Flores, Daniela Echaide, Ignacio Eduardo Suarez, Carlos Esteban Schnittger, Leonhard The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title | The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title_full | The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title_fullStr | The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title_full_unstemmed | The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title_short | The glycosylphosphatidylinositol-anchored protein repertoire of Babesia bovis and its significance for erythrocyte invasion |
| title_sort | glycosylphosphatidylinositol anchored protein repertoire of babesia bovis and its significance for erythrocyte invasion |
| topic | Babesia Bovis Eritrocitos Erythrocytes Babesiosis Glycosylphosphatidylinositol |
| url | http://hdl.handle.net/20.500.12123/1755 https://www.sciencedirect.com/science/article/pii/S1877959X1400034X https://doi.org/10.1016/j.ttbdis.2013.12.011 |
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