Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina
The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae family was detected by different PCR assays. The phylogenetic analyses of the obta...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/17543 https://www.sciencedirect.com/science/article/pii/S0147957123000383 https://doi.org/10.1016/j.cimid.2023.101980 |
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| author | Sebastian, Patrick Mazzucco Panizza, Matilde Nahimé García Ríos, Ignacio José María Tarragona, Evelina Luisa Trova, Gabriela Beatriz Sánchez Negrette, Olga Primo, María Evangelina Nava, Santiago |
| author_browse | García Ríos, Ignacio José María Mazzucco Panizza, Matilde Nahimé Nava, Santiago Primo, María Evangelina Sebastian, Patrick Sánchez Negrette, Olga Tarragona, Evelina Luisa Trova, Gabriela Beatriz |
| author_facet | Sebastian, Patrick Mazzucco Panizza, Matilde Nahimé García Ríos, Ignacio José María Tarragona, Evelina Luisa Trova, Gabriela Beatriz Sánchez Negrette, Olga Primo, María Evangelina Nava, Santiago |
| author_sort | Sebastian, Patrick |
| collection | INTA Digital |
| description | The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae family was detected by different PCR assays. The phylogenetic analyses of the obtained partial DNA sequences of the 16 S rDNA gene resulted in the identification of two different Anaplasma spp.: (I) Anaplasma platys-like bacteria (in blood sample from cattle and pools of R. microplus larvae and (II) Candidatus Anaplasma boleense (in blood samples from goats and one pool of R. microplus larvae of R. microplus). Candidatus A. boleense was found in two provinces that belong to different biogeographic regions, which leads to the conclusion that this bacterium may be widely distributed in Argentina. Interestingly, both Anaplasma spp. were found in the same R. microplus population in Chaco province, indicating that these two strains of Anaplasma are circulating in the same tick population. The results of this work represent the first report of the circulation of A. platys-like bacteria and Ca. A. boleense in domestic ruminants and free-living R. microplus ticks in Argentina. Further studies to determine the prevalence of infection, dispersion, clinical impact, transmission routes and cross-reactivity in serological tests of both Anaplasma species are needed. |
| format | Artículo |
| id | INTA17543 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA175432024-04-25T14:22:27Z Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina Sebastian, Patrick Mazzucco Panizza, Matilde Nahimé García Ríos, Ignacio José María Tarragona, Evelina Luisa Trova, Gabriela Beatriz Sánchez Negrette, Olga Primo, María Evangelina Nava, Santiago Livestock Domestic Animals Metastigmata Ganado Animales Domésticos Argentina Anaplasma Rhipicephalus Rhipicephalus microplus Garrapatas Ticks The present study aimed at the molecular detection of Anaplasma spp. in different samples obtained from cattle, goats and free-living Rhipicephalus microplus ticks from Argentina. DNA of members of the Anaplasmataceae family was detected by different PCR assays. The phylogenetic analyses of the obtained partial DNA sequences of the 16 S rDNA gene resulted in the identification of two different Anaplasma spp.: (I) Anaplasma platys-like bacteria (in blood sample from cattle and pools of R. microplus larvae and (II) Candidatus Anaplasma boleense (in blood samples from goats and one pool of R. microplus larvae of R. microplus). Candidatus A. boleense was found in two provinces that belong to different biogeographic regions, which leads to the conclusion that this bacterium may be widely distributed in Argentina. Interestingly, both Anaplasma spp. were found in the same R. microplus population in Chaco province, indicating that these two strains of Anaplasma are circulating in the same tick population. The results of this work represent the first report of the circulation of A. platys-like bacteria and Ca. A. boleense in domestic ruminants and free-living R. microplus ticks in Argentina. Further studies to determine the prevalence of infection, dispersion, clinical impact, transmission routes and cross-reactivity in serological tests of both Anaplasma species are needed. EEA Rafaela Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Mazzucco Panizza, Matilde Nahimé. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: García Ríos, Ignacio José María. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: García Ríos, Ignacio José María. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Trova, Gabriela Beatriz. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Trova, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Sánchez Negrette, Olga. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Sánchez Negrette, Olga. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina 2024-04-25T13:57:17Z 2024-04-25T13:57:17Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17543 https://www.sciencedirect.com/science/article/pii/S0147957123000383 0147-9571 https://doi.org/10.1016/j.cimid.2023.101980 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 96 : 101980 (May 2023) |
| spellingShingle | Livestock Domestic Animals Metastigmata Ganado Animales Domésticos Argentina Anaplasma Rhipicephalus Rhipicephalus microplus Garrapatas Ticks Sebastian, Patrick Mazzucco Panizza, Matilde Nahimé García Ríos, Ignacio José María Tarragona, Evelina Luisa Trova, Gabriela Beatriz Sánchez Negrette, Olga Primo, María Evangelina Nava, Santiago Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title | Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title_full | Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title_fullStr | Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title_full_unstemmed | Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title_short | Molecular detection and phylogenetic analysis of Anaplasma platys-like and Candidatus Anaplasma boleense strains from Argentina |
| title_sort | molecular detection and phylogenetic analysis of anaplasma platys like and candidatus anaplasma boleense strains from argentina |
| topic | Livestock Domestic Animals Metastigmata Ganado Animales Domésticos Argentina Anaplasma Rhipicephalus Rhipicephalus microplus Garrapatas Ticks |
| url | http://hdl.handle.net/20.500.12123/17543 https://www.sciencedirect.com/science/article/pii/S0147957123000383 https://doi.org/10.1016/j.cimid.2023.101980 |
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