A safe and effective vaccine against bovine leukemia virus
Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based on an attenuated BLV provirus by deleting genes dispensable for infectivity bu...
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/17466 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.980514/full https://doi.org/10.3389/fimmu.2022.980514 |
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| author | Suarez Archilla, Guillermo Gutierrez, Gerónimo Camussone, Cecilia Calvinho, Luis Fernando Abdala, Alejandro Ariel Alvarez, Irene Petersen, Marcos Iván Franco, Lautaro Nahuel De Stefano, Gabriel Alejandro Monti, Gustavo Jacques, Jean-Rock Joris, Thomas Willems, Luc Trono, Karina Gabriela |
| author_browse | Abdala, Alejandro Ariel Alvarez, Irene Calvinho, Luis Fernando Camussone, Cecilia De Stefano, Gabriel Alejandro Franco, Lautaro Nahuel Gutierrez, Gerónimo Jacques, Jean-Rock Joris, Thomas Monti, Gustavo Petersen, Marcos Iván Suarez Archilla, Guillermo Trono, Karina Gabriela Willems, Luc |
| author_facet | Suarez Archilla, Guillermo Gutierrez, Gerónimo Camussone, Cecilia Calvinho, Luis Fernando Abdala, Alejandro Ariel Alvarez, Irene Petersen, Marcos Iván Franco, Lautaro Nahuel De Stefano, Gabriel Alejandro Monti, Gustavo Jacques, Jean-Rock Joris, Thomas Willems, Luc Trono, Karina Gabriela |
| author_sort | Suarez Archilla, Guillermo |
| collection | INTA Digital |
| description | Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based on an attenuated BLV provirus by deleting genes dispensable for infectivity but required for efficient replication. The ability of the vaccine to protect from natural BLV infection was investigated in the context of dairy productive conditions in an endemic region. The attenuated vaccine was tested in a farm in which the prevalence rose from 16.7% in young cattle at the beginning of the study to more than 90% in adult individuals. Sterilizing immunity was obtained in 28 out of 29 vaccinated heifers over a period of 48 months, demonstrating the effectiveness of the vaccine. As indicated by the antiviral antibody titers, the humoral response was slightly reduced compared to wild-type infection. After initial post-vaccination bursts, the proviral loads of the attenuated vaccine remained most frequently undetectable. During the first dairy cycle, proviral DNA was not detected by nested-PCR in milk samples from vaccinated cows. During the second dairy cycle, provirus was sporadically detected in milk of two vaccinated cows. Forty-two calves born from vaccinated cows were negative for proviral DNA but had antiviral antibodies in their peripheral blood. The attenuated strain was not transmitted to sentinels, further supporting the safety of the vaccine. Altogether, these data thus demonstrate that the vaccine against BLV is safe and effective in herd conditions characterized by a very high incidence. This cost-effective approach will thus decrease the prevalence of BLV without modification of production practices. After facing a series of challenges pertaining to effectiveness and biosafety, the vaccine is now available for further large-scale delivery. The different challenges and hurdles that were bypassed may be informative for the development of a vaccine against HTLV-1. |
| format | Artículo |
| id | INTA17466 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA174662025-12-26T13:32:50Z A safe and effective vaccine against bovine leukemia virus Suarez Archilla, Guillermo Gutierrez, Gerónimo Camussone, Cecilia Calvinho, Luis Fernando Abdala, Alejandro Ariel Alvarez, Irene Petersen, Marcos Iván Franco, Lautaro Nahuel De Stefano, Gabriel Alejandro Monti, Gustavo Jacques, Jean-Rock Joris, Thomas Willems, Luc Trono, Karina Gabriela Vaccines Bovine Leukemia Virus Vacuna Virus Leucemia Bovina BLV Retrovirus HTLV Cepa Atenuada Attenuated Strain Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based on an attenuated BLV provirus by deleting genes dispensable for infectivity but required for efficient replication. The ability of the vaccine to protect from natural BLV infection was investigated in the context of dairy productive conditions in an endemic region. The attenuated vaccine was tested in a farm in which the prevalence rose from 16.7% in young cattle at the beginning of the study to more than 90% in adult individuals. Sterilizing immunity was obtained in 28 out of 29 vaccinated heifers over a period of 48 months, demonstrating the effectiveness of the vaccine. As indicated by the antiviral antibody titers, the humoral response was slightly reduced compared to wild-type infection. After initial post-vaccination bursts, the proviral loads of the attenuated vaccine remained most frequently undetectable. During the first dairy cycle, proviral DNA was not detected by nested-PCR in milk samples from vaccinated cows. During the second dairy cycle, provirus was sporadically detected in milk of two vaccinated cows. Forty-two calves born from vaccinated cows were negative for proviral DNA but had antiviral antibodies in their peripheral blood. The attenuated strain was not transmitted to sentinels, further supporting the safety of the vaccine. Altogether, these data thus demonstrate that the vaccine against BLV is safe and effective in herd conditions characterized by a very high incidence. This cost-effective approach will thus decrease the prevalence of BLV without modification of production practices. After facing a series of challenges pertaining to effectiveness and biosafety, the vaccine is now available for further large-scale delivery. The different challenges and hurdles that were bypassed may be informative for the development of a vaccine against HTLV-1. EEA Rafaela Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Gutierrez, Gerónimo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Calvinho, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Calvinho, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Abdala, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Abdala, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Petersen, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Petersen, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Monti, Gustavo. Wageningen University and Research. Quantitative Veterinary Epidemiology Group; Países Bajos Fil: Jacques, Jean-Rock. University of Liège. Molecular and Cellular Epigenetics (GIGA) and Molecular Biology (TERRA); Bélgica Fil: Jacques, Jean-Rock. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA); Bélgica Fil: Joris, Thomas. University of Liège. Molecular and Cellular Epigenetics (GIGA) and Molecular Biology (TERRA); Bélgica Fil: Joris, Thomas. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA); Bélgica Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics (GIGA) and Molecular Biology (TERRA); Bélgica Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA); Bélgica Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-04-19T10:43:21Z 2024-04-19T10:43:21Z 2022-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17466 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.980514/full 1664-3224 (electronic) https://doi.org/10.3389/fimmu.2022.980514 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I113-001, Abordaje integral para la conservación, mejoramiento y rescate de especies amenazadas de importancia para el SAAA en diferentes ambientes info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Immunology 13 : 980514 (2022) |
| spellingShingle | Vaccines Bovine Leukemia Virus Vacuna Virus Leucemia Bovina BLV Retrovirus HTLV Cepa Atenuada Attenuated Strain Suarez Archilla, Guillermo Gutierrez, Gerónimo Camussone, Cecilia Calvinho, Luis Fernando Abdala, Alejandro Ariel Alvarez, Irene Petersen, Marcos Iván Franco, Lautaro Nahuel De Stefano, Gabriel Alejandro Monti, Gustavo Jacques, Jean-Rock Joris, Thomas Willems, Luc Trono, Karina Gabriela A safe and effective vaccine against bovine leukemia virus |
| title | A safe and effective vaccine against bovine leukemia virus |
| title_full | A safe and effective vaccine against bovine leukemia virus |
| title_fullStr | A safe and effective vaccine against bovine leukemia virus |
| title_full_unstemmed | A safe and effective vaccine against bovine leukemia virus |
| title_short | A safe and effective vaccine against bovine leukemia virus |
| title_sort | safe and effective vaccine against bovine leukemia virus |
| topic | Vaccines Bovine Leukemia Virus Vacuna Virus Leucemia Bovina BLV Retrovirus HTLV Cepa Atenuada Attenuated Strain |
| url | http://hdl.handle.net/20.500.12123/17466 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.980514/full https://doi.org/10.3389/fimmu.2022.980514 |
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