Role of "well known" proteins on cell wall degradation and softening
Trabajo presentado al V International Conference Postharvest Unlimited, Chipre, 2015
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
International Society for Horticultural Science
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/17434 https://doi.org/10.17660/ActaHortic.2015.1079.5 |
| _version_ | 1855037666253340672 |
|---|---|
| author | Vicente, Ariel Roberto Rodoni, Luis Maria Salato, Gloria Silvia Basanta, Maria Florencia Ponce, Nora Marta Andrea Stortz, Carlos Arturo Raffo Benegas, Maria Dolores Powell, Ann L.T. Bennett, A.B. Labavitch, John Marcus |
| author_browse | Basanta, Maria Florencia Bennett, A.B. Labavitch, John Marcus Ponce, Nora Marta Andrea Powell, Ann L.T. Raffo Benegas, Maria Dolores Rodoni, Luis Maria Salato, Gloria Silvia Stortz, Carlos Arturo Vicente, Ariel Roberto |
| author_facet | Vicente, Ariel Roberto Rodoni, Luis Maria Salato, Gloria Silvia Basanta, Maria Florencia Ponce, Nora Marta Andrea Stortz, Carlos Arturo Raffo Benegas, Maria Dolores Powell, Ann L.T. Bennett, A.B. Labavitch, John Marcus |
| author_sort | Vicente, Ariel Roberto |
| collection | INTA Digital |
| description | Trabajo presentado al V International Conference Postharvest Unlimited, Chipre, 2015 |
| format | info:ar-repo/semantics/artículo |
| id | INTA17434 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Society for Horticultural Science |
| publisherStr | International Society for Horticultural Science |
| record_format | dspace |
| spelling | INTA174342024-04-17T12:05:46Z Role of "well known" proteins on cell wall degradation and softening Vicente, Ariel Roberto Rodoni, Luis Maria Salato, Gloria Silvia Basanta, Maria Florencia Ponce, Nora Marta Andrea Stortz, Carlos Arturo Raffo Benegas, Maria Dolores Powell, Ann L.T. Bennett, A.B. Labavitch, John Marcus Fruits Cell Walls Polygalacturonase Frutas Pared Celular Poligalacturonasa Softening Expansin Reblandecimiento Expansina Trabajo presentado al V International Conference Postharvest Unlimited, Chipre, 2015 Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and extent of their wall disassembly was quite similar. No marked pectin or hemicellulose depolymerization was observed and a similar reduction in tightly bound pectins and hemicellulose was detected in both cultivars during ripening. However, firm cherries presented pectic polymers with lower proportion of neutral sugars compared to uronic acids, suggesting that the variation in total wall polysaccharide and the branching of pectins assembled early in development or the proportion of homogalacturonan (HG) to type rhamnogalacturonan-I (RG-I) may contribute to the differences in firmness between cultivars. Dismantling of the cell wall by the action of relatively “well known” loosening agents is involved in the progressive softening occurring during ripening. Two of these proteins include polygalacturonases (PG) which are known to hydrolyze homogalacturonans and expansins (Exp) believed to participate in the relaxation of the cell wall by reducing hydrogen bonding between cellulose microfibrils and xyloglucan. We investigated the in vivo roles of these wall-disassembling proteins, by overexpressing PG and Exp1 both alone and in combination in a non-ripening rin tomato background. The simultaneous overexpression of PG and Exp1 in rin fruit restored softening in these non-ripening fruit. Unexpectedly, PG overexpression resulted in higher hemicellulose depolymerization while increased levels of Exp1 accelerated pectin turnover. This shows that besides their “well known” in vitro functions these proteins act in muro by facilitating the degradation of non directly targeted wall components, likely by increasing the accessibility of pre-existing wall-degrading proteins to their polysaccharide substrates. EEA Alto Valle Fil: Vicente, Ariel Roberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Rodoni, Luis Maria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Salato, Gloria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Basanta, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Raffo Benegas, María Dolores. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina Fil: Powell, Ann L.T. University of California. Department of Plant Sciences, Estados Unidos Fil: Bennett, A.B. University of California. Department of Plant Sciences, Estados Unidos Fil: Labavitch, John Marcus. University of California. Department of Plant Sciences, Estados Unidos 2024-04-17T11:53:17Z 2024-04-17T11:53:17Z 2015-03-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17434 0567-7572 https://doi.org/10.17660/ActaHortic.2015.1079.5 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf International Society for Horticultural Science Acta Horticulturae 1079 : 61-69 (2015) |
| spellingShingle | Fruits Cell Walls Polygalacturonase Frutas Pared Celular Poligalacturonasa Softening Expansin Reblandecimiento Expansina Vicente, Ariel Roberto Rodoni, Luis Maria Salato, Gloria Silvia Basanta, Maria Florencia Ponce, Nora Marta Andrea Stortz, Carlos Arturo Raffo Benegas, Maria Dolores Powell, Ann L.T. Bennett, A.B. Labavitch, John Marcus Role of "well known" proteins on cell wall degradation and softening |
| title | Role of "well known" proteins on cell wall degradation and softening |
| title_full | Role of "well known" proteins on cell wall degradation and softening |
| title_fullStr | Role of "well known" proteins on cell wall degradation and softening |
| title_full_unstemmed | Role of "well known" proteins on cell wall degradation and softening |
| title_short | Role of "well known" proteins on cell wall degradation and softening |
| title_sort | role of well known proteins on cell wall degradation and softening |
| topic | Fruits Cell Walls Polygalacturonase Frutas Pared Celular Poligalacturonasa Softening Expansin Reblandecimiento Expansina |
| url | http://hdl.handle.net/20.500.12123/17434 https://doi.org/10.17660/ActaHortic.2015.1079.5 |
| work_keys_str_mv | AT vicentearielroberto roleofwellknownproteinsoncellwalldegradationandsoftening AT rodoniluismaria roleofwellknownproteinsoncellwalldegradationandsoftening AT salatogloriasilvia roleofwellknownproteinsoncellwalldegradationandsoftening AT basantamariaflorencia roleofwellknownproteinsoncellwalldegradationandsoftening AT poncenoramartaandrea roleofwellknownproteinsoncellwalldegradationandsoftening AT stortzcarlosarturo roleofwellknownproteinsoncellwalldegradationandsoftening AT raffobenegasmariadolores roleofwellknownproteinsoncellwalldegradationandsoftening AT powellannlt roleofwellknownproteinsoncellwalldegradationandsoftening AT bennettab roleofwellknownproteinsoncellwalldegradationandsoftening AT labavitchjohnmarcus roleofwellknownproteinsoncellwalldegradationandsoftening |