Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test
The role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one of the major hosts of the virus that can develop persistent asymptomatic infection highlights the importance of sustaining surveillance on the antibody response elicited by vaccination in these animals. There is gap in t...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/17408 https://www.frontiersin.org/articles/10.3389/fvets.2023.1162477/full https://doi.org/10.3389/fvets.2023.1162477 |
| _version_ | 1855485954983198720 |
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| author | Sala, Juan Manuel Mansilla, Florencia Celeste Miraglia, Maria Cruz Caspe, Sergio Gaston Perez Filgueira, Daniel Mariano Capozzo, Alejandra |
| author_browse | Capozzo, Alejandra Caspe, Sergio Gaston Mansilla, Florencia Celeste Miraglia, Maria Cruz Perez Filgueira, Daniel Mariano Sala, Juan Manuel |
| author_facet | Sala, Juan Manuel Mansilla, Florencia Celeste Miraglia, Maria Cruz Caspe, Sergio Gaston Perez Filgueira, Daniel Mariano Capozzo, Alejandra |
| author_sort | Sala, Juan Manuel |
| collection | INTA Digital |
| description | The role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one of the major hosts of the virus that can develop persistent asymptomatic infection highlights the importance of sustaining surveillance on the antibody response elicited by vaccination in these animals. There is gap in the knowledge on how serological assays that measure antibodies against capsid proteins perform with buffalo samples and which would be the most reliable test to substitute the virus neutralization test (VNT) a cumbersome and low-throughput tool for field surveillance. Alternatively, the liquid-phase blocking sandwich ELISA (LPBE) is commonly used. Previous data from our laboratory demonstrated that the vaccine-induced antibodies assessed by the LPBE yielded low specificity with buffaloes’ samples. In contrast, a single-dilution avidity ELISA (AE) aimed to detect high-avidity antibodies against exposed epitopes, combined with an indirect ELISA (IE) to assess IgG levels, produced more reliable results. Here we analyzed for the first time the kinetics of the antibodies induced by vaccination in two different buffalo herds (n = 91) over 120 days using AE, IE, LPBE, and the VNT. Kinetics were similar in the different assays, with an increase of antibodies between 0- and 14-days post-vaccination (dpv) which were maintained thereafter. VNT and AE results were concordant (Kappa value = 0.76), and both assays revealed a decay in the antibody response in calves with maternal antibodies at 90 and 120 dpv, which was not evidenced by the LPBE. These results show that kinetics of antibody responses to FMD vaccination are similar in buffalo and cattle, and support the use of indirect ELISA assays, in particular Avidity ELISA, as alternatives to the VNT for vaccine-immunity monitoring irrespectively of the animal’s passive or active immune status. |
| format | Artículo |
| id | INTA17408 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA174082024-04-15T11:42:58Z Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test Sala, Juan Manuel Mansilla, Florencia Celeste Miraglia, Maria Cruz Caspe, Sergio Gaston Perez Filgueira, Daniel Mariano Capozzo, Alejandra Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Anticuerpos Serología Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines ELISA Antibodies Serology The role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one of the major hosts of the virus that can develop persistent asymptomatic infection highlights the importance of sustaining surveillance on the antibody response elicited by vaccination in these animals. There is gap in the knowledge on how serological assays that measure antibodies against capsid proteins perform with buffalo samples and which would be the most reliable test to substitute the virus neutralization test (VNT) a cumbersome and low-throughput tool for field surveillance. Alternatively, the liquid-phase blocking sandwich ELISA (LPBE) is commonly used. Previous data from our laboratory demonstrated that the vaccine-induced antibodies assessed by the LPBE yielded low specificity with buffaloes’ samples. In contrast, a single-dilution avidity ELISA (AE) aimed to detect high-avidity antibodies against exposed epitopes, combined with an indirect ELISA (IE) to assess IgG levels, produced more reliable results. Here we analyzed for the first time the kinetics of the antibodies induced by vaccination in two different buffalo herds (n = 91) over 120 days using AE, IE, LPBE, and the VNT. Kinetics were similar in the different assays, with an increase of antibodies between 0- and 14-days post-vaccination (dpv) which were maintained thereafter. VNT and AE results were concordant (Kappa value = 0.76), and both assays revealed a decay in the antibody response in calves with maternal antibodies at 90 and 120 dpv, which was not evidenced by the LPBE. These results show that kinetics of antibody responses to FMD vaccination are similar in buffalo and cattle, and support the use of indirect ELISA assays, in particular Avidity ELISA, as alternatives to the VNT for vaccine-immunity monitoring irrespectively of the animal’s passive or active immune status. EEA Mercedes Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentina 2024-04-15T11:39:13Z 2024-04-15T11:39:13Z 2023-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17408 https://www.frontiersin.org/articles/10.3389/fvets.2023.1162477/full 2297-1769 https://doi.org/10.3389/fvets.2023.1162477 eng info:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 10 : 1162477. (November 2023) |
| spellingShingle | Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Anticuerpos Serología Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines ELISA Antibodies Serology Sala, Juan Manuel Mansilla, Florencia Celeste Miraglia, Maria Cruz Caspe, Sergio Gaston Perez Filgueira, Daniel Mariano Capozzo, Alejandra Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title | Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title_full | Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title_fullStr | Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title_full_unstemmed | Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title_short | Kinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization test |
| title_sort | kinetics of foot and mouth disease vaccine induced antibody responses in buffaloes bubalus bubalis avidity elisa as an alternative to the virus neutralization test |
| topic | Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Anticuerpos Serología Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines ELISA Antibodies Serology |
| url | http://hdl.handle.net/20.500.12123/17408 https://www.frontiersin.org/articles/10.3389/fvets.2023.1162477/full https://doi.org/10.3389/fvets.2023.1162477 |
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