Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/17381 https://link.springer.com/article/10.1007/s00709-023-01922-x https://doi.org/10.1007/s00709-023-01922-x |
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| author | Palacios, María Belén Rizzo, Alex Joel Heredia, Tatiana Belén Roqueiro, Gonzalo Maldonado, Sara Murgida, Daniel Horacio Burrieza, Hernán Pablo |
| author_browse | Burrieza, Hernán Pablo Heredia, Tatiana Belén Maldonado, Sara Murgida, Daniel Horacio Palacios, María Belén Rizzo, Alex Joel Roqueiro, Gonzalo |
| author_facet | Palacios, María Belén Rizzo, Alex Joel Heredia, Tatiana Belén Roqueiro, Gonzalo Maldonado, Sara Murgida, Daniel Horacio Burrieza, Hernán Pablo |
| author_sort | Palacios, María Belén |
| collection | INTA Digital |
| description | Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA17381 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA173812024-04-11T12:37:49Z Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress Palacios, María Belén Rizzo, Alex Joel Heredia, Tatiana Belén Roqueiro, Gonzalo Maldonado, Sara Murgida, Daniel Horacio Burrieza, Hernán Pablo Chenopodium quinoa Estrés Osmótico Osmotic Strees Quinoa Quinua Estrés Salino Salt Stress Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation. EEA San Juan Fil: Palacios, María Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Palacios, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rizzo, Alex Joel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Rizzo, Alex Joel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heredia, Tatiana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heredia, Tatiana Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Maldonado, Sara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Maldonado, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil. Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica Analítica y Química Física. INQUIMAE; Argentina Fil: Burrieza, Hernán Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Burrieza, Hernán Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-04-11T12:20:44Z 2024-04-11T12:20:44Z 2024-01-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17381 https://link.springer.com/article/10.1007/s00709-023-01922-x 1615-6102 0033-183X https://doi.org/10.1007/s00709-023-01922-x eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Protoplasma : 1-15. (Published: 13 January 2024) |
| spellingShingle | Chenopodium quinoa Estrés Osmótico Osmotic Strees Quinoa Quinua Estrés Salino Salt Stress Palacios, María Belén Rizzo, Alex Joel Heredia, Tatiana Belén Roqueiro, Gonzalo Maldonado, Sara Murgida, Daniel Horacio Burrieza, Hernán Pablo Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title | Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title_full | Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title_fullStr | Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title_full_unstemmed | Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title_short | Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| title_sort | structure ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress |
| topic | Chenopodium quinoa Estrés Osmótico Osmotic Strees Quinoa Quinua Estrés Salino Salt Stress |
| url | http://hdl.handle.net/20.500.12123/17381 https://link.springer.com/article/10.1007/s00709-023-01922-x https://doi.org/10.1007/s00709-023-01922-x |
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