Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Cera...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Wiley
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/17329 https://onlinelibrary.wiley.com/doi/10.1111/eea.13417 https://doi.org/10.1111/eea.13417 |
| _version_ | 1855037646175207424 |
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| author | Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando |
| author_browse | Bachmann, Guillermo Enrique Devescovi, Francisco Fernández, Patricia Nussenbaum, Ana Laura Segura, Diego Fernando |
| author_facet | Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando |
| author_sort | Devescovi, Francisco |
| collection | INTA Digital |
| description | Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA17329 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA173292024-04-08T14:14:42Z Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes. Instituto de Genética Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Devescovi, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Química de Biomoléculas; Argentina Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Fernández, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bachmann, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2024-04-08T13:43:06Z 2024-04-08T13:43:06Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17329 https://onlinelibrary.wiley.com/doi/10.1111/eea.13417 1570-7458 https://doi.org/10.1111/eea.13417 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Entomologia Experimentalis et Applicata : 1–11 (First published: 06 February 2024) |
| spellingShingle | Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title | Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title_full | Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title_fullStr | Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title_full_unstemmed | Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title_short | Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata |
| title_sort | direct and indirect host related volatile compounds attract a fruit fly parasitoid diachasmimorpha longicaudata |
| topic | Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada |
| url | http://hdl.handle.net/20.500.12123/17329 https://onlinelibrary.wiley.com/doi/10.1111/eea.13417 https://doi.org/10.1111/eea.13417 |
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