Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers
Neospora caninum is an obligate intracellular parasite that causes abortion in ruminants. Different strains produce differences in the severity of disease outcomes. These differences may cause physiological or pathological changes in cells, modifying the intercellular interactions and intracellular...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/17321 https://www.sciencedirect.com/science/article/pii/S0021997524000288 https://doi.org/10.1016/j.jcpa.2024.02.006 |
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| author | Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Della Rosa, Paola Ortega-Mora, Luis M. Bacigalupe, Diana Venturini, María Cecilia Campero, Carlos Manuel Barbeito, Claudio Gustavo |
| author_browse | Bacigalupe, Diana Barbeito, Claudio Gustavo Campero, Carlos Manuel Caspe, Sergio Gaston Della Rosa, Paola Konrad, Jose Luis Moore, Prando Dadin Ortega-Mora, Luis M. Sala, Juan Manuel Venturini, María Cecilia |
| author_facet | Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Della Rosa, Paola Ortega-Mora, Luis M. Bacigalupe, Diana Venturini, María Cecilia Campero, Carlos Manuel Barbeito, Claudio Gustavo |
| author_sort | Caspe, Sergio Gaston |
| collection | INTA Digital |
| description | Neospora caninum is an obligate intracellular parasite that causes abortion in ruminants. Different strains produce differences in the severity of disease outcomes. These differences may cause physiological or pathological changes in cells, modifying the intercellular interactions and intracellular transport pathways that could be evidenced by identifying the terminal sugars. This study aimed to characterize the oligosaccharide pattern in the bovine placenta and uterus after infection with tachyzoites of three different strains of N. caninum (Nc-1, Nc-6 Argentina and Nc Spain-7) during early gestation. Fourteen heifers were inoculated intravenously on day 70 of gestation with 2 × 108 N. caninum tachyzoites and samples of placentae and uteri were analysed by histology and lectin histochemistry. In the infected groups, severe placentitis was associated with changes in lectin binding in the vascular endothelium by Lens culinaris agglutinin (LCA), Pisum sativum agglutinin (PSA) and Ricinus communis I (RCA-I) lectins, in the epithelial cells of the endometrial glands by RCA-I, Dolichos biflorus agglutinin (DBA), succinylated wheat germ agglutinin, peanut agglutinin (PNA), concanavalin-A (CON-A), LCA, PSA and Phaseolus vulgaris erythroagglutinin (PHA-e), and in the trophoblast layer by PNA, CON-A, LCA, PSA, PHA-e, soybean agglutinin, RCA-I, DBA and Bandieraea simplicifolia agglutinin (BSA-I). The results suggest that N. caninum causes changes in the glycosylation pattern in the maternofetal interface tissues and might cause abortions in early gestation due to changes in the cellular structure of the placenta. |
| format | info:ar-repo/semantics/artículo |
| id | INTA17321 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA173212024-04-08T11:24:05Z Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Della Rosa, Paola Ortega-Mora, Luis M. Bacigalupe, Diana Venturini, María Cecilia Campero, Carlos Manuel Barbeito, Claudio Gustavo Neospora caninum Cattle Heifers Placenta Infection Glycosides Animal Diseases Ganado Bovino Novilla Infección Glicosidos Enfermedades de los Animales Neospora caninum is an obligate intracellular parasite that causes abortion in ruminants. Different strains produce differences in the severity of disease outcomes. These differences may cause physiological or pathological changes in cells, modifying the intercellular interactions and intracellular transport pathways that could be evidenced by identifying the terminal sugars. This study aimed to characterize the oligosaccharide pattern in the bovine placenta and uterus after infection with tachyzoites of three different strains of N. caninum (Nc-1, Nc-6 Argentina and Nc Spain-7) during early gestation. Fourteen heifers were inoculated intravenously on day 70 of gestation with 2 × 108 N. caninum tachyzoites and samples of placentae and uteri were analysed by histology and lectin histochemistry. In the infected groups, severe placentitis was associated with changes in lectin binding in the vascular endothelium by Lens culinaris agglutinin (LCA), Pisum sativum agglutinin (PSA) and Ricinus communis I (RCA-I) lectins, in the epithelial cells of the endometrial glands by RCA-I, Dolichos biflorus agglutinin (DBA), succinylated wheat germ agglutinin, peanut agglutinin (PNA), concanavalin-A (CON-A), LCA, PSA and Phaseolus vulgaris erythroagglutinin (PHA-e), and in the trophoblast layer by PNA, CON-A, LCA, PSA, PHA-e, soybean agglutinin, RCA-I, DBA and Bandieraea simplicifolia agglutinin (BSA-I). The results suggest that N. caninum causes changes in the glycosylation pattern in the maternofetal interface tissues and might cause abortions in early gestation due to changes in the cellular structure of the placenta. EEA Mercedes Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Konrad, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Konrad, Jose Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Della Rosa, Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Ortega-Mora, Luis M. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Bacigalupe, Diana. Municipalidad de Rauch. Departamento de Zoonosis; Argentina Fil: Venturini, María C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Campero, Carlos Manuel. Moredun Research Institute; Reino Unido Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-04-08T11:17:41Z 2024-04-08T11:17:41Z 2024-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17321 https://www.sciencedirect.com/science/article/pii/S0021997524000288 0021-9975 1532-3129 https://doi.org/10.1016/j.jcpa.2024.02.006 eng info:eu-repograntAgreement/INTA/PNSA-1115053/AR./Biotecnologías reproductivas y desarrollo de metodologías de diagnóstico, control y prevención de las enfermedades infecciosas y parasitarias que afectan la concepción, gestación y período neonatal en especies de interés zootécnico. info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of Comparative Pathology 210 : 29-37. (April 2024) |
| spellingShingle | Neospora caninum Cattle Heifers Placenta Infection Glycosides Animal Diseases Ganado Bovino Novilla Infección Glicosidos Enfermedades de los Animales Caspe, Sergio Gaston Konrad, Jose Luis Moore, Prando Dadin Sala, Juan Manuel Della Rosa, Paola Ortega-Mora, Luis M. Bacigalupe, Diana Venturini, María Cecilia Campero, Carlos Manuel Barbeito, Claudio Gustavo Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title | Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title_full | Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title_fullStr | Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title_full_unstemmed | Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title_short | Infection with different Neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of Neospora caninum-infected heifers |
| title_sort | infection with different neospora caninum strains causes differences in the glycosylation pattern in the uteri and placentae of neospora caninum infected heifers |
| topic | Neospora caninum Cattle Heifers Placenta Infection Glycosides Animal Diseases Ganado Bovino Novilla Infección Glicosidos Enfermedades de los Animales |
| url | http://hdl.handle.net/20.500.12123/17321 https://www.sciencedirect.com/science/article/pii/S0021997524000288 https://doi.org/10.1016/j.jcpa.2024.02.006 |
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