Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina
Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal pr...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1727 https://link.springer.com/article/10.1007/s10658-012-9951-2 https://doi.org/10.1007/s10658-012-9951-2 |
| _version_ | 1855034845517840384 |
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| author | Fernandez, Franco Daniel Conci, Vilma Cecilia Kirschbaum, Daniel Santiago Conci, Luis Rogelio |
| author_browse | Conci, Luis Rogelio Conci, Vilma Cecilia Fernandez, Franco Daniel Kirschbaum, Daniel Santiago |
| author_facet | Fernandez, Franco Daniel Conci, Vilma Cecilia Kirschbaum, Daniel Santiago Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99–98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches’ broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwide |
| format | info:ar-repo/semantics/artículo |
| id | INTA1727 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA17272018-06-29T12:14:03Z Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina Fernandez, Franco Daniel Conci, Vilma Cecilia Kirschbaum, Daniel Santiago Conci, Luis Rogelio Fresa Fitoplasmas Biología Molecular Strawberries Phytoplasmas Molecular Biology Frutilla Argentina Strawberry (Fragaria x ananassa Duch. cv. Camino Real) plants showing phyllody symptoms were detected in production fields in Bella Vista (Corrientes province, Argentina). DNA from all symptomatic samples analyzed yielded fragments of the expected size in PCR reactions using phytoplasma universal primers. DNA from asymptomatic plants yielded no products. The associated phytoplasma was designated as Argentinean Strawberry Phyllody (ASP). The ASP sequence of the 16S rRNA gene showed 99–98% homology with members of the 16SrVII ash yellows group. The putative RFLP profile was indistinguishable from the Argentinean alfalfa witches’ broom (ArAWB) phytoplasma. The phylogenetic analysis of nearly full-length 16S rDNA sequence and 16S-23S spacer region yielded a consensus tree wherein ASP clustered into the ash yellows group (16Sr VII) with high confidence values (95), generating a separated branch (100 bootstrap value) together with ArAWB (16Sr VII-C). In Argentina, the 16Sr VII group was also detected in alfalfa (Medicago sativa L.) crops, and weeds [Artemisa annua L. and Conyza bonariensis (L.) Cronquist]. To our knowledge, this is the first report of a phytoplasma from the 16Sr VII group affecting strawberries in Argentina and worldwide Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kirschbaum, Daniel Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba; Argentina 2017-11-09T13:20:48Z 2017-11-09T13:20:48Z 2013-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1727 https://link.springer.com/article/10.1007/s10658-012-9951-2 0929-1873 (Print) 1573-8469 (Online) https://doi.org/10.1007/s10658-012-9951-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) European journal of plant pathology 135 (1) : 1–4. (January 2013) |
| spellingShingle | Fresa Fitoplasmas Biología Molecular Strawberries Phytoplasmas Molecular Biology Frutilla Argentina Fernandez, Franco Daniel Conci, Vilma Cecilia Kirschbaum, Daniel Santiago Conci, Luis Rogelio Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title | Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title_full | Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title_fullStr | Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title_full_unstemmed | Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title_short | Molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry (Fragaria x ananassa Duch.) plants in Argentina |
| title_sort | molecular characterization of a phytoplasma of the ash yellows group occurring in strawberry fragaria x ananassa duch plants in argentina |
| topic | Fresa Fitoplasmas Biología Molecular Strawberries Phytoplasmas Molecular Biology Frutilla Argentina |
| url | http://hdl.handle.net/20.500.12123/1727 https://link.springer.com/article/10.1007/s10658-012-9951-2 https://doi.org/10.1007/s10658-012-9951-2 |
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