Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina
Strawberry red leaf phytoplasma was found in strawberry plants from production fields in Lules (Tucumán province) and Bella Vista (Corrientes province), Argentina. Characteristic strawberry red leaf symptoms were stunting, young leaves with yellowing at the edges, mature leaves which curled and were...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1725 http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/8/2741_ijs000276.pdf?expires=1510232001&id=id&accname=guest&checksum=225A4A05DA639BDAD277850E46C653C7 |
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| author | Fernandez, Franco Daniel Meneguzzi, Natalia Guzman, Fabiana Kirschbaum, Daniel Santiago Conci, Vilma Cecilia Nome Docampo, Claudia Conci, Luis Rogelio |
| author_browse | Conci, Luis Rogelio Conci, Vilma Cecilia Fernandez, Franco Daniel Guzman, Fabiana Kirschbaum, Daniel Santiago Meneguzzi, Natalia Nome Docampo, Claudia |
| author_facet | Fernandez, Franco Daniel Meneguzzi, Natalia Guzman, Fabiana Kirschbaum, Daniel Santiago Conci, Vilma Cecilia Nome Docampo, Claudia Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Strawberry red leaf phytoplasma was found in strawberry plants from production fields in Lules (Tucumán province) and Bella Vista (Corrientes province), Argentina. Characteristic strawberry red leaf symptoms were stunting, young leaves with yellowing at the edges, mature leaves which curled and were reddish at the abaxial face, flower and fruit deformation and death. The pathogen was detected with phytoplasma-universal primer pairs P1/P7 followed by R16F2n/R16R2 as nested primers in 13 diseased plants. Based on RFLP and sequence analysis of the amplified 16S rRNA gene, the phytoplasma was related to the 16SrXIII group (Mexican periwinkle virescence). In silico the RFLP profile of all the samples analysed revealed the presence of a unique pattern, showing that the novel phytoplasma is different from all the phytoplasmas currently composing the 16SrXIII group. The phylogenetic analysis was consistent with RFLP analysis as the strawberry red leaf phytoplasma was grouped within the 16SrXIII group, but formed a particular cluster. On this basis, the Strawberry red leaf phytoplasma associated with strawberry red leaf disease was assigned to a new subgroup, 16SrXIII-F. |
| format | Artículo |
| id | INTA1725 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA17252022-04-20T16:07:08Z Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina Fernandez, Franco Daniel Meneguzzi, Natalia Guzman, Fabiana Kirschbaum, Daniel Santiago Conci, Vilma Cecilia Nome Docampo, Claudia Conci, Luis Rogelio Fresa Fitoplasmas Identificación Enfermedades de las Plantas Strawberries Phytoplasmas Identification Plant Diseases Frutilla Argentina Strawberry red leaf phytoplasma was found in strawberry plants from production fields in Lules (Tucumán province) and Bella Vista (Corrientes province), Argentina. Characteristic strawberry red leaf symptoms were stunting, young leaves with yellowing at the edges, mature leaves which curled and were reddish at the abaxial face, flower and fruit deformation and death. The pathogen was detected with phytoplasma-universal primer pairs P1/P7 followed by R16F2n/R16R2 as nested primers in 13 diseased plants. Based on RFLP and sequence analysis of the amplified 16S rRNA gene, the phytoplasma was related to the 16SrXIII group (Mexican periwinkle virescence). In silico the RFLP profile of all the samples analysed revealed the presence of a unique pattern, showing that the novel phytoplasma is different from all the phytoplasmas currently composing the 16SrXIII group. The phylogenetic analysis was consistent with RFLP analysis as the strawberry red leaf phytoplasma was grouped within the 16SrXIII group, but formed a particular cluster. On this basis, the Strawberry red leaf phytoplasma associated with strawberry red leaf disease was assigned to a new subgroup, 16SrXIII-F. Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Meneguzzi, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Kirschbaum, Daniel Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba; Argentina 2017-11-09T12:52:45Z 2017-11-09T12:52:45Z 2015-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1725 http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/8/2741_ijs000276.pdf?expires=1510232001&id=id&accname=guest&checksum=225A4A05DA639BDAD277850E46C653C7 1466-5026 (Print) 1466-5034 (Online) doi: 10.1099/ijs.0.000276 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina (nation) International Journal of Systematic and Evolutionary Microbiology 65 (8) : 2741-2747 (August 2015) |
| spellingShingle | Fresa Fitoplasmas Identificación Enfermedades de las Plantas Strawberries Phytoplasmas Identification Plant Diseases Frutilla Argentina Fernandez, Franco Daniel Meneguzzi, Natalia Guzman, Fabiana Kirschbaum, Daniel Santiago Conci, Vilma Cecilia Nome Docampo, Claudia Conci, Luis Rogelio Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title | Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title_full | Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title_fullStr | Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title_full_unstemmed | Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title_short | Detection and identification of a novel 16SrXIII subgroup phytoplasma associated with strawberry red leaf disease in Argentina |
| title_sort | detection and identification of a novel 16srxiii subgroup phytoplasma associated with strawberry red leaf disease in argentina |
| topic | Fresa Fitoplasmas Identificación Enfermedades de las Plantas Strawberries Phytoplasmas Identification Plant Diseases Frutilla Argentina |
| url | http://hdl.handle.net/20.500.12123/1725 http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/8/2741_ijs000276.pdf?expires=1510232001&id=id&accname=guest&checksum=225A4A05DA639BDAD277850E46C653C7 |
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