Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata
Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspect...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BioMed Central
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/17219 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-6 https://doi.org/10.1186/s12864-024-10034-6 |
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| author | Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz |
| author_browse | Lanzavecchia, Silvia Beatriz Latorre Estivalis, Jose M. Segura, Diego Fernando Traverso, Lucila M. Wulff, Juan Pedro |
| author_facet | Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz |
| author_sort | Wulff, Juan Pedro |
| collection | INTA Digital |
| description | Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition.
Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males.
Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes. |
| format | Artículo |
| id | INTA17219 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA172192024-03-26T14:34:29Z Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata Background: Diachasmimorpha longicaudata is a hymenopteran fruit fly endoparasitoid. Females of this species find their hosts for oviposition by using complex sensorial mechanisms in response to physical and chemical stimuli associated with the host and host habitat. Ecological and behavioral aspects related to host-seeking behavior for oviposition have been extensively studied in D. longicaudata, including the identification of volatile organic compounds acting as attractants to females. In this sense, molecular mechanisms of chemoreception have been explored in this species, including a preliminary characterization of odorant-binding proteins (OBPs), chemosensory proteins (CSPs) and odorant receptors (ORs), among other proteins. Functional assays on OBP and CSP have been conducted as a first approach to identify molecular mechanisms associated with the female host-seeking behavior for oviposition. The aims of the present study were to identify the D. longicaudata sensory gene repertoire expressed in the antenna of sexually mature and mated individuals of both sexes, and subsequently, characterize transcripts differentially expressed in the antennae of females to identify candidate genes associated with the female host-seeking behavior for oviposition. Results: A total of 33,745 predicted protein-coding sequences were obtained from a de novo antennal transcriptome assembly. Ten sensory-related gene families were annotated as follows: 222 ORs, 44 ionotropic receptors (IRs), 25 gustatory receptors (GRs), 9 CSPs, 13 OBPs, 2 ammonium transporters (AMTs), 8 pickpocket (PPKs) receptors, 16 transient receptor potential (TRP) channels, 12 CD36/SNMPs and 3 Niemann-Pick type C2 like proteins (NPC2-like). The differential expression analysis revealed 237 and 151 transcripts up- and downregulated, respectively, between the female and male antennae. Ninety-seven differentially expressed transcripts corresponded to sensory-related genes including 88 transcripts being upregulated (87 ORs and one TRP) and nine downregulated (six ORs, two CSPs and one OBP) in females compared to males. Conclusions: The sensory gene repertoire of D. longicaudata was similar to that of other taxonomically related parasitoid wasps. We identified a high number of ORs upregulated in the female antenna. These results may indicate that this gene family has a central role in the chemoreception of sexually mature females during the search for hosts and host habitats for reproductive purposes. Instituto de Genética Fil: Wulff, Juan Pedro. North Carolina State University. Entomology and Plant Pathology; Estados Unidos Fil: Traverso, Lucila M. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos. Laboratorio de Neurobiología de Insectos; Argentina Fil: Traverso, Lucila M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología. Laboratorio de Insectos Sociales, Biología Molecular y Neurociencias; Argentina Fil: Latorre Estivalis, Jose M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina 2024-03-26T14:27:26Z 2024-03-26T14:27:26Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17219 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-6 1471-2164 https://doi.org/10.1186/s12864-024-10034-6 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC Genomics 25 (1) : 147 (Febrero 2024) |
| spellingShingle | Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata Wulff, Juan Pedro Traverso, Lucila M. Latorre Estivalis, Jose M. Segura, Diego Fernando Lanzavecchia, Silvia Beatriz Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title | Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title_full | Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title_fullStr | Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title_full_unstemmed | Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title_short | Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata |
| title_sort | identification of candidate genes associated with host seeking behavior in the parasitoid wasp diachasmimorpha longicaudata |
| topic | Candidate Genes Gene Expression Receptors Parasitoids Tephritidae Genes Candidatos Expresión Génica Receptor Parasitoides Biosteres longicaudatus Secuencia de ARN RNA Sequence Diachasmimorpha longicaudata |
| url | http://hdl.handle.net/20.500.12123/17219 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10034-6 https://doi.org/10.1186/s12864-024-10034-6 |
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