Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, id...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Taylor and Francis
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/17119 https://www.tandfonline.com/doi/10.1080/15324982.2023.2233933 https://doi.org/10.1080/15324982.2023.2233933 |
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| author | Carrasco, Franca Denise Miranda, Victoria Sede, Silvana M. Bustos, Sebastian González, Valeria Otero, Maria Laura Fracchia, Sebastián |
| author_browse | Bustos, Sebastian Carrasco, Franca Denise Fracchia, Sebastián González, Valeria Miranda, Victoria Otero, Maria Laura Sede, Silvana M. |
| author_facet | Carrasco, Franca Denise Miranda, Victoria Sede, Silvana M. Bustos, Sebastian González, Valeria Otero, Maria Laura Fracchia, Sebastián |
| author_sort | Carrasco, Franca Denise |
| collection | INTA Digital |
| description | Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments. |
| format | Artículo |
| id | INTA17119 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Taylor and Francis |
| publisherStr | Taylor and Francis |
| record_format | dspace |
| spelling | INTA171192024-03-20T10:46:17Z Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae Carrasco, Franca Denise Miranda, Victoria Sede, Silvana M. Bustos, Sebastian González, Valeria Otero, Maria Laura Fracchia, Sebastián Biological Control Semi-arid Zones Control Biológico Zona Semiárida Verticillium dahliae Trichoderma Olea europaea Biocontrol Olive Disease Root Endophyte Semiarid Arid Soils Olivo Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments. Instituto de Patología Vegetal Fil: Carrasco, Franca Denise. Instituto Nacional de Tecnologıa Agropecuaria (INTA).Estacion Experimental Agropecuaria Catamarca; Argentina Fil: Miranda, Victoria. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de la Rioja (CRILAR).Consejo Nacional de Investigaciones Científicas y Técnicas- Gobierno de La Rioja. Universidad Nacional de Catamarca.Servicio Geológico Minero Argentino.Universidad Nacional de La Rioja; Argentina Fil: Sede, Silvana M. Instituto de Bot anica Darwinion (IBODA). Academia Nacional de Ciencias Exactas, Físicas y Naturales (ANCEFN).Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Bustos, Sebastian. Universidad Nacional de Catamarca (UNCa). Facultad de Ciencias Agrarias; Argentina Fil: González, Valeria. Universidad Nacional de C ordoba (UNC). Facultad de Ciencias Agropecuarias (FCA); Argentina Fil: Otero, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Otero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Fracchia, Sebastián. Universidad de Buenos Aires. Facultas de Ciencias Exactas y Naturales. Instituto de Micologıa y Botanica (INMIBO); Argentina 2024-03-20T10:40:20Z 2024-03-20T10:40:20Z 2023-07-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17119 https://www.tandfonline.com/doi/10.1080/15324982.2023.2233933 1532-4982 1532-4990 https://doi.org/10.1080/15324982.2023.2233933 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor and Francis Arid Land Research and Management 38 (1) : 122-143 (2024) |
| spellingShingle | Biological Control Semi-arid Zones Control Biológico Zona Semiárida Verticillium dahliae Trichoderma Olea europaea Biocontrol Olive Disease Root Endophyte Semiarid Arid Soils Olivo Carrasco, Franca Denise Miranda, Victoria Sede, Silvana M. Bustos, Sebastian González, Valeria Otero, Maria Laura Fracchia, Sebastián Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title | Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title_full | Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title_fullStr | Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title_full_unstemmed | Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title_short | Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae |
| title_sort | screening for native trichoderma strains as potential biocontrollers of the olive pathogen verticillium dahliae |
| topic | Biological Control Semi-arid Zones Control Biológico Zona Semiárida Verticillium dahliae Trichoderma Olea europaea Biocontrol Olive Disease Root Endophyte Semiarid Arid Soils Olivo |
| url | http://hdl.handle.net/20.500.12123/17119 https://www.tandfonline.com/doi/10.1080/15324982.2023.2233933 https://doi.org/10.1080/15324982.2023.2233933 |
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