Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples
Killer yeasts are frequently used to combat and prevent contamination by wild-type yeasts during wine production and they can even dominate the wine fermentation. Stuck and sluggish fermentations can be caused by an unbalanced ratio of killer to sensitive yeasts in the bioreactor, and therefore it i...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1706 https://link.springer.com/article/10.1007%2Fs11274-012-1123-1 https://doi.org/10.1007/s11274-012-1123-1 |
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| author | Maturano, Yolanda Paola Nally, María Cristina Toro, María Eugenia Castellanos de Figueroa, Lucía Inés Combina, Mariana Vazquez, Fabio |
| author_browse | Castellanos de Figueroa, Lucía Inés Combina, Mariana Maturano, Yolanda Paola Nally, María Cristina Toro, María Eugenia Vazquez, Fabio |
| author_facet | Maturano, Yolanda Paola Nally, María Cristina Toro, María Eugenia Castellanos de Figueroa, Lucía Inés Combina, Mariana Vazquez, Fabio |
| author_sort | Maturano, Yolanda Paola |
| collection | INTA Digital |
| description | Killer yeasts are frequently used to combat and prevent contamination by wild-type yeasts during wine production and they can even dominate the wine fermentation. Stuck and sluggish fermentations can be caused by an unbalanced ratio of killer to sensitive yeasts in the bioreactor, and therefore it is important to determine the proportion of both populations. The aim of this study was to provide a simple tool to monitor killer yeast populations during controlled mixed microvinifications of killer and sensitive Saccharomyces cerevisiae. Samples were periodically extracted during vinification, seeded on Petri dishes and incubated at 25 and 37 °C; the latter temperature was assayed for possible inactivation of killer toxin production. Colonies developed under the described conditions were randomly transferred to killer phenotype detection medium. Significant differences in the killer/sensitive ratio were observed between both incubation temperatures in all microvinifications. These results suggest that 37 °C seems a better option to determine the biomass of sensitive yeasts, in order to avoid underestimation of sensitive cells in the presence of killer yeasts during fermentations. Incubation at a toxin-inhibiting temperature clearly showed the real ratio of killer to sensitive cells in fermentation systems. |
| format | Artículo |
| id | INTA1706 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA17062017-11-21T12:54:04Z Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples Maturano, Yolanda Paola Nally, María Cristina Toro, María Eugenia Castellanos de Figueroa, Lucía Inés Combina, Mariana Vazquez, Fabio Levadura Temperatura Vinificación Yeasts Temperature Winemaking Killer yeasts are frequently used to combat and prevent contamination by wild-type yeasts during wine production and they can even dominate the wine fermentation. Stuck and sluggish fermentations can be caused by an unbalanced ratio of killer to sensitive yeasts in the bioreactor, and therefore it is important to determine the proportion of both populations. The aim of this study was to provide a simple tool to monitor killer yeast populations during controlled mixed microvinifications of killer and sensitive Saccharomyces cerevisiae. Samples were periodically extracted during vinification, seeded on Petri dishes and incubated at 25 and 37 °C; the latter temperature was assayed for possible inactivation of killer toxin production. Colonies developed under the described conditions were randomly transferred to killer phenotype detection medium. Significant differences in the killer/sensitive ratio were observed between both incubation temperatures in all microvinifications. These results suggest that 37 °C seems a better option to determine the biomass of sensitive yeasts, in order to avoid underestimation of sensitive cells in the presence of killer yeasts during fermentations. Incubation at a toxin-inhibiting temperature clearly showed the real ratio of killer to sensitive cells in fermentation systems. Fil: Maturano, Yolanda Paola. Universidad Nacional de San Juan. Instituto de Biotecnología; Argentina Fil: Nally, María Cristina. Universidad Nacional de San Juan. Instituto de Biotecnología; Argentina Fil: Toro, María Eugenia. Universidad Nacional de San Juan. Instituto de Biotecnología; Argentina Fil: Castellanos de Figueroa, Lucía Inés. PROIMI; Argentina. Universidad Nacional de Tucumán. FBQyF; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Fabio. Universidad Nacional de San Juan. Instituto de Biotecnología; Argentina 2017-11-08T12:38:43Z 2017-11-08T12:38:43Z 2012-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1706 https://link.springer.com/article/10.1007%2Fs11274-012-1123-1 0959-3993 (Print) 1573-0972 (Online) https://doi.org/10.1007/s11274-012-1123-1 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf World Journal of Microbiology and Biotechnology 28 (11) : 3135–3142 (November 2012) |
| spellingShingle | Levadura Temperatura Vinificación Yeasts Temperature Winemaking Maturano, Yolanda Paola Nally, María Cristina Toro, María Eugenia Castellanos de Figueroa, Lucía Inés Combina, Mariana Vazquez, Fabio Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title | Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title_full | Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title_fullStr | Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title_full_unstemmed | Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title_short | Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples |
| title_sort | monitoring of killer yeast populations in mixed cultures influence of incubation temperature of microvinifications samples |
| topic | Levadura Temperatura Vinificación Yeasts Temperature Winemaking |
| url | http://hdl.handle.net/20.500.12123/1706 https://link.springer.com/article/10.1007%2Fs11274-012-1123-1 https://doi.org/10.1007/s11274-012-1123-1 |
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