Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells
Varicellovirus bovinealpha 1 (formerly bovine alphaherpesvirus type 1, BoAHV-1) is associated with several syndromes in cattle, including respiratory disease and is one of the main agents involved in the bovine respiratory disease complex (BRDC). Its infectious cycle is characterized by latent infec...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/17008 https://link.springer.com/article/10.1007/s00203-023-03806-3 https://doi.org/10.1007/s00203-023-03806-3 |
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| author | Pérez, Sandra Miró, María Victoria Verna, Andrea Elizabeth González Altamiranda, Erika Barcos, Oscar Lanusse, Carlos Edmundo Lifschitz, Adrián Luis |
| author_browse | Barcos, Oscar González Altamiranda, Erika Lanusse, Carlos Edmundo Lifschitz, Adrián Luis Miró, María Victoria Pérez, Sandra Verna, Andrea Elizabeth |
| author_facet | Pérez, Sandra Miró, María Victoria Verna, Andrea Elizabeth González Altamiranda, Erika Barcos, Oscar Lanusse, Carlos Edmundo Lifschitz, Adrián Luis |
| author_sort | Pérez, Sandra |
| collection | INTA Digital |
| description | Varicellovirus bovinealpha 1 (formerly bovine alphaherpesvirus type 1, BoAHV-1) is associated with several syndromes in cattle, including respiratory disease and is one of the main agents involved in the bovine respiratory disease complex (BRDC). Its infectious cycle is characterized by latent infections with sporadic virus reactivation and transmission. Although the acute disease can be prevented by the use of vaccines, specific therapeutic measures are not available. Ivermectin (IVM) is a semi-synthetic avermectin with a broad-spectrum antiparasitic activity, which has previously shown to have potential as an antiviral drug. In this study, IVM antiviral activity against BoAHV-1 was characterized in two cell lines (MDBK [Madin Darby bovine kidney] and BT [bovine turbinate]), including the measurement of intracellular drug accumulation within virus-infected cells. IVM antiviral activity was assessed at three different drug concentrations (1.25, 2.5 and 5 µM) after incubation for 24, 48 and 72 h. Slight cytotoxicity was only observed with 5 µM IVM. Even the lowest IVM dose was able to induce a significant reduction in virus titers in both cell lines. These findings indicate that the antiviral effects of IVM were evident in our experimental model within the range of concentrations achievable through therapeutic in vivo administration. Consequently, additional in vivo trials are necessary to validate the potential utility of these results in effectively managing BoAHV-1 in infected cattle. |
| format | Artículo |
| id | INTA17008 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA170082024-09-24T11:08:02Z Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells Pérez, Sandra Miró, María Victoria Verna, Andrea Elizabeth González Altamiranda, Erika Barcos, Oscar Lanusse, Carlos Edmundo Lifschitz, Adrián Luis Ganado Bovino Herpes Virus Bovino Ivermectina Medicamentos Cattle Bovine Herpesvirus Ivermectin Herpesviridae Drugs Varicellovirus bovinealpha 1 (formerly bovine alphaherpesvirus type 1, BoAHV-1) is associated with several syndromes in cattle, including respiratory disease and is one of the main agents involved in the bovine respiratory disease complex (BRDC). Its infectious cycle is characterized by latent infections with sporadic virus reactivation and transmission. Although the acute disease can be prevented by the use of vaccines, specific therapeutic measures are not available. Ivermectin (IVM) is a semi-synthetic avermectin with a broad-spectrum antiparasitic activity, which has previously shown to have potential as an antiviral drug. In this study, IVM antiviral activity against BoAHV-1 was characterized in two cell lines (MDBK [Madin Darby bovine kidney] and BT [bovine turbinate]), including the measurement of intracellular drug accumulation within virus-infected cells. IVM antiviral activity was assessed at three different drug concentrations (1.25, 2.5 and 5 µM) after incubation for 24, 48 and 72 h. Slight cytotoxicity was only observed with 5 µM IVM. Even the lowest IVM dose was able to induce a significant reduction in virus titers in both cell lines. These findings indicate that the antiviral effects of IVM were evident in our experimental model within the range of concentrations achievable through therapeutic in vivo administration. Consequently, additional in vivo trials are necessary to validate the potential utility of these results in effectively managing BoAHV-1 in infected cattle. EEA Balcarce Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Miró, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Verna, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Gonzalez Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Barcos, Oscar. Laboratorio Colón; Argentina. Fil: Lanusse, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Lanusse, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Lifschitz, Adrián. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Lifschitz, Adrián. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2024-03-12T17:47:52Z 2024-03-12T17:47:52Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/pubishedVersion http://hdl.handle.net/20.500.12123/17008 https://link.springer.com/article/10.1007/s00203-023-03806-3 1432-072X (online) 0302-8933 (print) https://doi.org/10.1007/s00203-023-03806-3 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Archives of Microbiology 206 : 78 (January 2024). |
| spellingShingle | Ganado Bovino Herpes Virus Bovino Ivermectina Medicamentos Cattle Bovine Herpesvirus Ivermectin Herpesviridae Drugs Pérez, Sandra Miró, María Victoria Verna, Andrea Elizabeth González Altamiranda, Erika Barcos, Oscar Lanusse, Carlos Edmundo Lifschitz, Adrián Luis Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title | Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title_full | Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title_fullStr | Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title_full_unstemmed | Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title_short | Ivermectin antiviral activity against Varicellovirus bovinealpha 1: assessment of intracellular drug accumulation in virus‑infected cells |
| title_sort | ivermectin antiviral activity against varicellovirus bovinealpha 1 assessment of intracellular drug accumulation in virus infected cells |
| topic | Ganado Bovino Herpes Virus Bovino Ivermectina Medicamentos Cattle Bovine Herpesvirus Ivermectin Herpesviridae Drugs |
| url | http://hdl.handle.net/20.500.12123/17008 https://link.springer.com/article/10.1007/s00203-023-03806-3 https://doi.org/10.1007/s00203-023-03806-3 |
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