Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phyloge...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16975 https://link.springer.com/article/10.1007/s11056-024-10034-0 https://doi.org/10.1007/s11056-024-10034-0 |
| _version_ | 1855485866643816448 |
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| author | Aparicio, Alejandro Gabriel El Mujtar, Veronica Andrea Azpilicueta, Maria Marta Martinez Meier, Alejandro Mondino, Victor Alejandro |
| author_browse | Aparicio, Alejandro Gabriel Azpilicueta, Maria Marta El Mujtar, Veronica Andrea Martinez Meier, Alejandro Mondino, Victor Alejandro |
| author_facet | Aparicio, Alejandro Gabriel El Mujtar, Veronica Andrea Azpilicueta, Maria Marta Martinez Meier, Alejandro Mondino, Victor Alejandro |
| author_sort | Aparicio, Alejandro Gabriel |
| collection | INTA Digital |
| description | In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change. |
| format | Artículo |
| id | INTA16975 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA169752024-03-08T13:26:35Z Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson Aparicio, Alejandro Gabriel El Mujtar, Veronica Andrea Azpilicueta, Maria Marta Martinez Meier, Alejandro Mondino, Victor Alejandro Pinus ponderosa Variación Genética ADN Mitocondrial Crecimiento Argentina Genetic Variation Mitochondrial DNA Growth In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change. EEA Bariloche Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Martinez Meier, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Mondino, Victor Alejandro. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Esquel; Argentina 2024-03-08T13:23:00Z 2024-03-08T13:23:00Z 2024-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16975 https://link.springer.com/article/10.1007/s11056-024-10034-0 0169-4286 1573-5095 https://doi.org/10.1007/s11056-024-10034-0 eng info:eu-repograntAgreement/INTA/PNFOR-1104062/AR./Mejoramiento genético de especies forestales introducidas para usos de alto valor. info:eu-repograntAgreement/INTA/2019-PE-E6-I146-001, Mejoramiento genético de especies forestales cultivadas de rápido crecimiento: un desarrollo clave para el fortalecimiento de la foresto industria nacional info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Springer New Forests : 1-21 (Published: 05 March 2024) |
| spellingShingle | Pinus ponderosa Variación Genética ADN Mitocondrial Crecimiento Argentina Genetic Variation Mitochondrial DNA Growth Aparicio, Alejandro Gabriel El Mujtar, Veronica Andrea Azpilicueta, Maria Marta Martinez Meier, Alejandro Mondino, Victor Alejandro Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title | Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title_full | Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title_fullStr | Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title_full_unstemmed | Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title_short | Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson |
| title_sort | mitochondrial dna lineages and genetic variation of early growth traits in the argentinean breeding population of pinus ponderosa douglas ex c lawson |
| topic | Pinus ponderosa Variación Genética ADN Mitocondrial Crecimiento Argentina Genetic Variation Mitochondrial DNA Growth |
| url | http://hdl.handle.net/20.500.12123/16975 https://link.springer.com/article/10.1007/s11056-024-10034-0 https://doi.org/10.1007/s11056-024-10034-0 |
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