Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs
In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were ide...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1694 http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb20 http://dx.doi.org/10.1080/03601234.2012.706558 |
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| author | Pizzolitto, Romina P. Armando, María R. Combina, Mariana Cavaglieri, Lilia Reneé Dalcero, Ana María Salvano, Mario A. |
| author_browse | Armando, María R. Cavaglieri, Lilia Reneé Combina, Mariana Dalcero, Ana María Pizzolitto, Romina P. Salvano, Mario A. |
| author_facet | Pizzolitto, Romina P. Armando, María R. Combina, Mariana Cavaglieri, Lilia Reneé Dalcero, Ana María Salvano, Mario A. |
| author_sort | Pizzolitto, Romina P. |
| collection | INTA Digital |
| description | In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host. |
| format | Artículo |
| id | INTA1694 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA16942019-01-23T17:17:42Z Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs Pizzolitto, Romina P. Armando, María R. Combina, Mariana Cavaglieri, Lilia Reneé Dalcero, Ana María Salvano, Mario A. Saccharomyces Cerevisiae Aflatoxinas Aves de Corral Piensos Aflatoxins Poultry Feeds Probiotics Probióticos In this study the aflatoxin B1 (AFB1) removal capacity, the tolerance to salivary and gastrointestinal conditions, autoaggregation and coaggregation with pathogenic bacteria of Saccharomyces cerevisiae strains isolated from broiler feces, were evaluated. Only four of twelve isolated strains were identified as Saccharomyces cerevisiae using molecular techniques. The results obtained in AFB1 binding studies indicated that the amount of AFB1 removed was both strain and mycotoxin-concentration dependent. Therefore, a theoretical model was applied in order to select the most efficient strain to remove AFB1 in a wide range of mycotoxin concentration. The results indicated that S. cerevisiae 08 and S. cerevisiae 01 strains were the most efficient microorganisms in the mycotoxin removal. Viability on simulated salivary and gastrointestinal conditions was investigated and S. cerevisiae 08 strain showed the best results, achieving 98% of total survival whereas S. cerevisiae 01 reached only 75%. Autoaggregation and coaggregation assays showed S. cerevisiae 08 as the most appropriate strain, mainly because it was the unique strain able to coaggregate with the four bacterial pathogens assayed. Consequently, S. cerevisiae 08 is the best candidate for future in vivo studies useful to prevent aflatoxicosis. Further quantitative in vitro and in vivo studies are required to evaluate the real impact of yeast-binding activity on the bioavailability of AFB1 in poultry. However, this study could be useful in selecting efficient strains in terms of AFB1 binding and provide an important contribution to research into microorganisms with potential probiotic effects on the host. Fil: Pizzolitto, Romina P. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Armando, María R. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Dalcero, Ana María. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Salvano, Mario A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Biología Molecular; Argentina 2017-11-07T13:53:06Z 2017-11-07T13:53:06Z 2012-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1694 http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb20 0360-1234 (Print) 1532-4109 (Online) http://dx.doi.org/10.1080/03601234.2012.706558 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of environmental science and health. Part B 47 (10) : 933-941. (2012) |
| spellingShingle | Saccharomyces Cerevisiae Aflatoxinas Aves de Corral Piensos Aflatoxins Poultry Feeds Probiotics Probióticos Pizzolitto, Romina P. Armando, María R. Combina, Mariana Cavaglieri, Lilia Reneé Dalcero, Ana María Salvano, Mario A. Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title | Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title_full | Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title_fullStr | Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title_full_unstemmed | Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title_short | Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs |
| title_sort | evaluation of saccharomyces cerevisiae strains as probiotic agent with aflatoxin b1 adsorption ability for use in poultry feedstuffs |
| topic | Saccharomyces Cerevisiae Aflatoxinas Aves de Corral Piensos Aflatoxins Poultry Feeds Probiotics Probióticos |
| url | http://hdl.handle.net/20.500.12123/1694 http://www.tandfonline.com/doi/abs/10.1080/03601234.2012.706558?journalCode=lesb20 http://dx.doi.org/10.1080/03601234.2012.706558 |
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