Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases
Campylobacter fetus spp. is a bacterium associated to reproductive losses in cattle worldwide. It is a venereal infectious disease known as bovine campilobacteriosis, with high impact mainly in countries with extensive production systems. Here, we show pathogenesis and diagnostic methods for Campylo...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer Nature
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16880 https://link.springer.com/article/10.1007/s11259-023-10193-z https://doi.org/10.1007/s11259-023-10193-z |
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| author | Garcia, Juan Agustin Farace, Pablo Daniel Gioffre, Andrea Romeo, Florencia Verna, Andrea Elizabeth Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Signorini Porchiett, Marcelo Lisandro Paolicchi, Fernando |
| author_browse | Aller Atucha, Juan Florencio Farace, Pablo Daniel Garcia, Juan Agustin Gioffre, Andrea Mendez, Maria Alejandra Morsella, Claudia Graciela Paolicchi, Fernando Romeo, Florencia Signorini Porchiett, Marcelo Lisandro Verna, Andrea Elizabeth |
| author_facet | Garcia, Juan Agustin Farace, Pablo Daniel Gioffre, Andrea Romeo, Florencia Verna, Andrea Elizabeth Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Signorini Porchiett, Marcelo Lisandro Paolicchi, Fernando |
| author_sort | Garcia, Juan Agustin |
| collection | INTA Digital |
| description | Campylobacter fetus spp. is a bacterium associated to reproductive losses in cattle worldwide. It is a venereal infectious disease known as bovine campilobacteriosis, with high impact mainly in countries with extensive production systems. Here, we show pathogenesis and diagnostic methods for Campylobacter fetus detection in cervico-vaginal mucus (CVM) samples from heifers experimentally infected and field cases from herds with low reproductive performance by campylobacteriosis infection. Bacterial culture, direct immunofluorescence test and qPCR were used as diagnostic methods to evaluate detection of C. fetus. In the experimental model 30 Aberdeen Angus and crossbred heifers and 4 Aberdeen Angus bulls for natural mating were assigned to 3 groups experimentally challenged with C. fetus subsp. fetus (Cff), C. fetus subsps venerealis (Cfv) and C. fetus subsp venerealis biovar intermedius (Cfvi), respectively, and a negative control group, all followed for 9 months. Also, field samples of CVM and aborted fetuses were recollected from seven beef cattle farms. Bacteriological culture had the higher C. fetus detection rate in CVM being the most appropriate, followed by qPCR (with commercial extraction DNA kit), direct immunofluorescence test and qPCR (with in-house extraction DNA method), in both, experimental model and field cases. From experimental model after natural mating, 62.5% and 25% heifers got pregnant from Cff and Cfvi groups, respectively, while from Cfv no pregnancy was detected. The strain more frequently detected was Cfvi, followed by Cff and Cfv. Colonization of Cff in female genital tract with high number of carriers and presence in aborted fetuses was evidenced, suggesting a high risk to bovine reproductive health. Bacteriemia was not detected after genital infection. Given the low detection rate of either test, we suggest the use of both, PCR based methods and bacterial culture could result in higher detection rate in farms with endemic campylobacteriosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16880 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA168802024-03-01T14:13:17Z Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases Garcia, Juan Agustin Farace, Pablo Daniel Gioffre, Andrea Romeo, Florencia Verna, Andrea Elizabeth Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Signorini Porchiett, Marcelo Lisandro Paolicchi, Fernando Campilobacteriosis Genital Bovina Rumiante Experimentación Enfermedades de Transmisión Sexual Bovine Genital Campylobacteriosis Ruminants Experimentation Sexually Transmitted Diseases Campylobacter fetus spp. is a bacterium associated to reproductive losses in cattle worldwide. It is a venereal infectious disease known as bovine campilobacteriosis, with high impact mainly in countries with extensive production systems. Here, we show pathogenesis and diagnostic methods for Campylobacter fetus detection in cervico-vaginal mucus (CVM) samples from heifers experimentally infected and field cases from herds with low reproductive performance by campylobacteriosis infection. Bacterial culture, direct immunofluorescence test and qPCR were used as diagnostic methods to evaluate detection of C. fetus. In the experimental model 30 Aberdeen Angus and crossbred heifers and 4 Aberdeen Angus bulls for natural mating were assigned to 3 groups experimentally challenged with C. fetus subsp. fetus (Cff), C. fetus subsps venerealis (Cfv) and C. fetus subsp venerealis biovar intermedius (Cfvi), respectively, and a negative control group, all followed for 9 months. Also, field samples of CVM and aborted fetuses were recollected from seven beef cattle farms. Bacteriological culture had the higher C. fetus detection rate in CVM being the most appropriate, followed by qPCR (with commercial extraction DNA kit), direct immunofluorescence test and qPCR (with in-house extraction DNA method), in both, experimental model and field cases. From experimental model after natural mating, 62.5% and 25% heifers got pregnant from Cff and Cfvi groups, respectively, while from Cfv no pregnancy was detected. The strain more frequently detected was Cfvi, followed by Cff and Cfv. Colonization of Cff in female genital tract with high number of carriers and presence in aborted fetuses was evidenced, suggesting a high risk to bovine reproductive health. Bacteriemia was not detected after genital infection. Given the low detection rate of either test, we suggest the use of both, PCR based methods and bacterial culture could result in higher detection rate in farms with endemic campylobacteriosis. EEA Balcarce Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Gioffré, Andrea. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Verna, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Méndez, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Signorini Porchiett, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Investigación de la Cadena Láctea; Argentina. Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2024-03-01T14:06:02Z 2024-03-01T14:06:02Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16880 https://link.springer.com/article/10.1007/s11259-023-10193-z 1573-7446 (Online) 0165-7380 (Print) https://doi.org/10.1007/s11259-023-10193-z eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Veterinary Research Communications 48 (1) : 113-124 (2024) |
| spellingShingle | Campilobacteriosis Genital Bovina Rumiante Experimentación Enfermedades de Transmisión Sexual Bovine Genital Campylobacteriosis Ruminants Experimentation Sexually Transmitted Diseases Garcia, Juan Agustin Farace, Pablo Daniel Gioffre, Andrea Romeo, Florencia Verna, Andrea Elizabeth Mendez, Maria Alejandra Morsella, Claudia Graciela Aller Atucha, Juan Florencio Signorini Porchiett, Marcelo Lisandro Paolicchi, Fernando Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title | Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title_full | Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title_fullStr | Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title_full_unstemmed | Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title_short | Bovine campylobacteriosis in heifer: pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| title_sort | bovine campylobacteriosis in heifer pathogenesis study and insights in the conventional and molecular diagnosis in an experimental bovine model and field cases |
| topic | Campilobacteriosis Genital Bovina Rumiante Experimentación Enfermedades de Transmisión Sexual Bovine Genital Campylobacteriosis Ruminants Experimentation Sexually Transmitted Diseases |
| url | http://hdl.handle.net/20.500.12123/16880 https://link.springer.com/article/10.1007/s11259-023-10193-z https://doi.org/10.1007/s11259-023-10193-z |
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