Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model
Limosilactobacillus fermentum Lf2 (Lf2) is an autochthonous strain that produces high levels of exopolysaccharides (EPS). The objective of this work was to evaluate the probiotic potential of Lf2 and its relationship with these metabolites in a mouse model of TNBS (trinitrobenzene sulfonic acid)-ind...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16875 https://www.mdpi.com/2311-5637/10/2/77 https://doi.org/10.3390/fermentation10020077 |
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| author | Ale, Elisa C. Irazoqui, Jose Matias Ale, Analia Peralta, Guillermo H. Puntillo, Melisa Burns, Patricia Graciela Correa Olivar, Gabriela Cazenave, Jimena Bergamini, Carina Viviana Amadio, Ariel Binetti, Ana |
| author_browse | Ale, Analia Ale, Elisa C. Amadio, Ariel Bergamini, Carina Viviana Binetti, Ana Burns, Patricia Graciela Cazenave, Jimena Correa Olivar, Gabriela Irazoqui, Jose Matias Peralta, Guillermo H. Puntillo, Melisa |
| author_facet | Ale, Elisa C. Irazoqui, Jose Matias Ale, Analia Peralta, Guillermo H. Puntillo, Melisa Burns, Patricia Graciela Correa Olivar, Gabriela Cazenave, Jimena Bergamini, Carina Viviana Amadio, Ariel Binetti, Ana |
| author_sort | Ale, Elisa C. |
| collection | INTA Digital |
| description | Limosilactobacillus fermentum Lf2 (Lf2) is an autochthonous strain that produces high levels of exopolysaccharides (EPS). The objective of this work was to evaluate the probiotic potential of Lf2 and its relationship with these metabolites in a mouse model of TNBS (trinitrobenzene sulfonic acid)-induced chronic colitis. Mice were treated intrarectally with increasing doses of TNBS resuspended in 50% ethanol for 14 days. In parallel, they received different treatments by gavage (lactose 10% as the matrix): freeze-dried Lf2 (L); purified EPS (E); and lactose 10% (T). A healthy control group (H) was treated with 50% alcohol without TNBS (intrarectally) and 10% lactose (by gavage). In the small intestine, there was a significant increase in IgA levels for the group that received EPS and a decrease in IFN-γ for mice treated with the strain compared to the other groups. In the large intestine, IL-2 and IFN-γ presented the lowest levels in the groups treated with EPS and the strain. The concentrations of acetic and propionic acids in mice that received Lf2 were the highest, while the levels of butyric acid were comparable to the healthy control group. An increase in the abundance of SCFA-producing bacteria was observed for mice treated with EPS and the strain in comparison with the colitis control group. The enzyme activity of catalase was higher in all the treatments compared to the TNBS-induced colitis control mice. To summarize the results obtained, a principal component analysis (PCA) was performed, clearly grouping the treatments in different clusters according to the variables studied. This is one of the first studies to address the role of a potential probiotic strain in a chronic colitis mouse model, trying to elucidate the relationship between its properties and the EPS synthesized. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16875 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA168752025-02-26T11:56:14Z Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model Ale, Elisa C. Irazoqui, Jose Matias Ale, Analia Peralta, Guillermo H. Puntillo, Melisa Burns, Patricia Graciela Correa Olivar, Gabriela Cazenave, Jimena Bergamini, Carina Viviana Amadio, Ariel Binetti, Ana Lactobacillus Exopolysaccharides Chronic Course Colitis Immunomodulation Exopolisacáridos Proceso Crónico Inmunomodulación Limosilactobacillus fermentum Limosilactobacillus fermentum Lf2 (Lf2) is an autochthonous strain that produces high levels of exopolysaccharides (EPS). The objective of this work was to evaluate the probiotic potential of Lf2 and its relationship with these metabolites in a mouse model of TNBS (trinitrobenzene sulfonic acid)-induced chronic colitis. Mice were treated intrarectally with increasing doses of TNBS resuspended in 50% ethanol for 14 days. In parallel, they received different treatments by gavage (lactose 10% as the matrix): freeze-dried Lf2 (L); purified EPS (E); and lactose 10% (T). A healthy control group (H) was treated with 50% alcohol without TNBS (intrarectally) and 10% lactose (by gavage). In the small intestine, there was a significant increase in IgA levels for the group that received EPS and a decrease in IFN-γ for mice treated with the strain compared to the other groups. In the large intestine, IL-2 and IFN-γ presented the lowest levels in the groups treated with EPS and the strain. The concentrations of acetic and propionic acids in mice that received Lf2 were the highest, while the levels of butyric acid were comparable to the healthy control group. An increase in the abundance of SCFA-producing bacteria was observed for mice treated with EPS and the strain in comparison with the colitis control group. The enzyme activity of catalase was higher in all the treatments compared to the TNBS-induced colitis control mice. To summarize the results obtained, a principal component analysis (PCA) was performed, clearly grouping the treatments in different clusters according to the variables studied. This is one of the first studies to address the role of a potential probiotic strain in a chronic colitis mouse model, trying to elucidate the relationship between its properties and the EPS synthesized. EEA Rafaela Fil: Ale, E.C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Ale, E.C. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Ale, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Nacional de Limnología; Argentina Fil: Ale, Analía. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Peralta, Guillermo H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Peralta, Guillermo H. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Puntillo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Puntillo, Melisa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina Fil: Burns, Patricia Graciela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina Fil: Correa Olivar, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Correa Olivar, Gabriela. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Nacional de Limnología; Argentina Fil: Cazenave, Jimena. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Bergamini, Carina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Bergamini, Carina Viviana. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Binetti, A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Binetti, Ana. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina 2024-03-01T13:29:03Z 2024-03-01T13:29:03Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16875 https://www.mdpi.com/2311-5637/10/2/77 2311-5637 https://doi.org/10.3390/fermentation10020077 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Fermentation 10 (2) : 77 (January 2024) |
| spellingShingle | Lactobacillus Exopolysaccharides Chronic Course Colitis Immunomodulation Exopolisacáridos Proceso Crónico Inmunomodulación Limosilactobacillus fermentum Ale, Elisa C. Irazoqui, Jose Matias Ale, Analia Peralta, Guillermo H. Puntillo, Melisa Burns, Patricia Graciela Correa Olivar, Gabriela Cazenave, Jimena Bergamini, Carina Viviana Amadio, Ariel Binetti, Ana Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title | Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title_full | Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title_fullStr | Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title_full_unstemmed | Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title_short | Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model |
| title_sort | protective role of limosilactobacillus fermentum lf2 and its exopolysaccharides eps in a tnbs induced chronic colitis mouse model |
| topic | Lactobacillus Exopolysaccharides Chronic Course Colitis Immunomodulation Exopolisacáridos Proceso Crónico Inmunomodulación Limosilactobacillus fermentum |
| url | http://hdl.handle.net/20.500.12123/16875 https://www.mdpi.com/2311-5637/10/2/77 https://doi.org/10.3390/fermentation10020077 |
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