Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos
Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16814 https://www.sciencedirect.com/science/article/pii/S0378113523002791 https://doi.org/10.1016/j.vetmic.2023.109925 |
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| author | Cagnoli, Claudia Inés Chiapparrone, María Laura Cacciato, Claudio Santiago Rodríguez, Marcelo Gastón Catena, María del Carmen Aller Atucha, Juan Florencio |
| author_browse | Aller Atucha, Juan Florencio Cacciato, Claudio Santiago Cagnoli, Claudia Inés Catena, María del Carmen Chiapparrone, María Laura Rodríguez, Marcelo Gastón |
| author_facet | Cagnoli, Claudia Inés Chiapparrone, María Laura Cacciato, Claudio Santiago Rodríguez, Marcelo Gastón Catena, María del Carmen Aller Atucha, Juan Florencio |
| author_sort | Cagnoli, Claudia Inés |
| collection | INTA Digital |
| description | Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16814 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA168142024-02-28T10:54:07Z Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos Cagnoli, Claudia Inés Chiapparrone, María Laura Cacciato, Claudio Santiago Rodríguez, Marcelo Gastón Catena, María del Carmen Aller Atucha, Juan Florencio Campylobacter Bovinae Pathogenesis Animal Embryos Animal Reproduction Patogénesis Embriones Animales In Vitro Reproducción Animal Campylobacter fetus is an important veterinary pathogen that causes campylobacteriosis. This disease causes decreased productivity of cattle by inducing reproductive losses. Although several virulence factors have been recognized in C. fetus, including a cytolethal distending toxin (CDT), the exact mechanism responsible for embryonic death remains unknown. The aim of this work was to evaluate the effect of C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv), and their toxin activity on the in vitro fertilization of bovine ova and early embryonic development. Two different experiments were performed. In Experiment 1, a total of 1524 cumulus–oocyte complexes (COCs) were inseminated, distributed into three groups: two of them infected with the microorganism (Cff, Cfv) and a control group. Percentages COCs cleaved were similar among groups (p = 0.1243); however, the embryonic development rate (blastocyst at day 7) in the control group was greater (p < 0.001) than those obtained in Cff and Cfv groups. In experiment 2, a total of 746 COCs were inseminated, divided into three groups: two of them treated with the bacterial-free culture filtrates to test toxin activity (Cff-CDT, Cfv-CDT) and a control group. Both cleavage and embryonic development rates were greater (p < 0.001) in the control group than those obtained in Cff-CDT and Cfv-CDT groups. This study provides evidence that both subspecies of C. fetus do not interfere with fertilization but do affect in vitro embryonic development. It is the first report on the biological effect of the CDT on bovine embryonic development. EEA Balcarce Fil: Cagnoli, Claudia Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina. Fil: Chiapparrone, María Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina. Fil: Cacciato, Claudio Santiago. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina. Fil: Rodríguez, Marcelo Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Catena, María del Carmen. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión de Investigaciones Cientificas; Argentina.Centro de Investigación Vaterinaria de Tandil; Argentina. Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. 2024-02-28T10:43:08Z 2024-02-28T10:43:08Z 2024-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16814 https://www.sciencedirect.com/science/article/pii/S0378113523002791 1873-2542 (online) 0378-1135 (print) https://doi.org/10.1016/j.vetmic.2023.109925 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Microbiology 288 : 109925 (January 2024) |
| spellingShingle | Campylobacter Bovinae Pathogenesis Animal Embryos Animal Reproduction Patogénesis Embriones Animales In Vitro Reproducción Animal Cagnoli, Claudia Inés Chiapparrone, María Laura Cacciato, Claudio Santiago Rodríguez, Marcelo Gastón Catena, María del Carmen Aller Atucha, Juan Florencio Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title | Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title_full | Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title_fullStr | Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title_full_unstemmed | Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title_short | Effect of Campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| title_sort | effect of campylobacter fetus on in vitro fertilization and embryonic development of preimplantation bovine embryos |
| topic | Campylobacter Bovinae Pathogenesis Animal Embryos Animal Reproduction Patogénesis Embriones Animales In Vitro Reproducción Animal |
| url | http://hdl.handle.net/20.500.12123/16814 https://www.sciencedirect.com/science/article/pii/S0378113523002791 https://doi.org/10.1016/j.vetmic.2023.109925 |
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