Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina

The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeog...

Full description

Bibliographic Details
Main Authors: Flores, Fernando Sebastian, Sebastian, Patrick Stephan, Nava, Santiago
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer 2024
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/16746
https://link.springer.com/article/10.1007/s11259-023-10219-6
https://doi.org/10.1007/s11259-023-10219-6
_version_ 1855037552975675392
author Flores, Fernando Sebastian
Sebastian, Patrick Stephan
Nava, Santiago
author_browse Flores, Fernando Sebastian
Nava, Santiago
Sebastian, Patrick Stephan
author_facet Flores, Fernando Sebastian
Sebastian, Patrick Stephan
Nava, Santiago
author_sort Flores, Fernando Sebastian
collection INTA Digital
description The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeographic Province. Molecular detection of order Rickettsiales agents was performed using gltA (Rickettsia spp.), 16 S rRNA and groEL (Anaplasmataceae) genes as targets. A total of 67 ticks of Amblyomma aureolatum (20 nymphs and 4 adults), Amblyomma dubitatum (15 nymphs) and Haemaphysalis juxtakochi (24 nymphs and 4 adults) were collected. While all tested ticks were negative for Rickettsia spp., and Anaplasmataceae agents could neither be detected in A. aureolatum nor in A. dubitatum, Candidatus Ehrlichia pampeana was detected in one male of H. juxtakochi. DNA sequences of this microorganism (16 S rDNA and groEL) are related to sequences of Ehrlichia ewingii. The findings of the current study represent the first report of this Ehrlichia strain for Argentina.
format info:ar-repo/semantics/artículo
id INTA16746
institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2024
publishDateRange 2024
publishDateSort 2024
publisher Springer
publisherStr Springer
record_format dspace
spelling INTA167462024-02-22T10:54:51Z Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina Flores, Fernando Sebastian Sebastian, Patrick Stephan Nava, Santiago Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeographic Province. Molecular detection of order Rickettsiales agents was performed using gltA (Rickettsia spp.), 16 S rRNA and groEL (Anaplasmataceae) genes as targets. A total of 67 ticks of Amblyomma aureolatum (20 nymphs and 4 adults), Amblyomma dubitatum (15 nymphs) and Haemaphysalis juxtakochi (24 nymphs and 4 adults) were collected. While all tested ticks were negative for Rickettsia spp., and Anaplasmataceae agents could neither be detected in A. aureolatum nor in A. dubitatum, Candidatus Ehrlichia pampeana was detected in one male of H. juxtakochi. DNA sequences of this microorganism (16 S rDNA and groEL) are related to sequences of Ehrlichia ewingii. The findings of the current study represent the first report of this Ehrlichia strain for Argentina. EEA Rafaela Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT); Argentina Fil: Flores, Fernando S. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina Fil: Sebastian, Patrick Stephan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2024-02-22T10:50:10Z 2024-02-22T10:50:10Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16746 https://link.springer.com/article/10.1007/s11259-023-10219-6 0165-7380 1573-7446 https://doi.org/10.1007/s11259-023-10219-6 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Springer Veterinary Research Communications 48 (1) : 585-589 (February 2024)
spellingShingle Ehrlichia
Ixodidae
Haemaphysalis
Amblyomma
Identification
Identificación
Argentina
Ehrlichia pampeana
Haemaphysalis juxtakochi
Garrapatas
Ticks
Flores, Fernando Sebastian
Sebastian, Patrick Stephan
Nava, Santiago
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title_full Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title_fullStr Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title_full_unstemmed Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title_short Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
title_sort molecular detection of candidatus ehrlichia pampeana rickettsiales anaplasmataceae in haemaphysalis juxtakochi acari ixodidae from central argentina
topic Ehrlichia
Ixodidae
Haemaphysalis
Amblyomma
Identification
Identificación
Argentina
Ehrlichia pampeana
Haemaphysalis juxtakochi
Garrapatas
Ticks
url http://hdl.handle.net/20.500.12123/16746
https://link.springer.com/article/10.1007/s11259-023-10219-6
https://doi.org/10.1007/s11259-023-10219-6
work_keys_str_mv AT floresfernandosebastian moleculardetectionofcandidatusehrlichiapampeanarickettsialesanaplasmataceaeinhaemaphysalisjuxtakochiacariixodidaefromcentralargentina
AT sebastianpatrickstephan moleculardetectionofcandidatusehrlichiapampeanarickettsialesanaplasmataceaeinhaemaphysalisjuxtakochiacariixodidaefromcentralargentina
AT navasantiago moleculardetectionofcandidatusehrlichiapampeanarickettsialesanaplasmataceaeinhaemaphysalisjuxtakochiacariixodidaefromcentralargentina