New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions amo...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16612 https://www.sciencedirect.com/science/article/pii/S1049964419304827 https://doi.org/10.1016/j.biocontrol.2019.104152 |
| _version_ | 1855037519726379008 |
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| author | Moya, Paulina Barrera, Viviana Andrea Cipollone, Josefina Bedoya, Carolina Kohan, Lucila Toledo, Andrea Sisterna, Marina |
| author_browse | Barrera, Viviana Andrea Bedoya, Carolina Cipollone, Josefina Kohan, Lucila Moya, Paulina Sisterna, Marina Toledo, Andrea |
| author_facet | Moya, Paulina Barrera, Viviana Andrea Cipollone, Josefina Bedoya, Carolina Kohan, Lucila Toledo, Andrea Sisterna, Marina |
| author_sort | Moya, Paulina |
| collection | INTA Digital |
| description | The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16612 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA166122024-02-15T11:22:52Z New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina Moya, Paulina Barrera, Viviana Andrea Cipollone, Josefina Bedoya, Carolina Kohan, Lucila Toledo, Andrea Sisterna, Marina Hongos Fungi Barley Trichoderma Pyrenophora teres Cebada Hypocreales Antagonistic Effect The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Moya, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Cipollone, Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Cipollone, Josefina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina Fil: Bedoya, Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Kohan, Lucila. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Toledo, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Toledo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Sisterna, Marina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina Fil: Sisterna, Marina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina 2024-02-15T11:11:18Z 2024-02-15T11:11:18Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16612 https://www.sciencedirect.com/science/article/pii/S1049964419304827 1090-2112 1049-9644 https://doi.org/10.1016/j.biocontrol.2019.104152 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Biological Control 141 : 104152 (Febrero 2020) |
| spellingShingle | Hongos Fungi Barley Trichoderma Pyrenophora teres Cebada Hypocreales Antagonistic Effect Moya, Paulina Barrera, Viviana Andrea Cipollone, Josefina Bedoya, Carolina Kohan, Lucila Toledo, Andrea Sisterna, Marina New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title | New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title_full | New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title_fullStr | New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title_full_unstemmed | New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title_short | New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina |
| title_sort | new isolates of trichoderma spp as biocontrol and plant growth promoting agents in the pathosystem pyrenophora teres barley in argentina |
| topic | Hongos Fungi Barley Trichoderma Pyrenophora teres Cebada Hypocreales Antagonistic Effect |
| url | http://hdl.handle.net/20.500.12123/16612 https://www.sciencedirect.com/science/article/pii/S1049964419304827 https://doi.org/10.1016/j.biocontrol.2019.104152 |
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