Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies
The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus Autographa californica multiple...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/16611 https://www.mdpi.com/2306-7381/8/11/278 https://doi.org/10.3390/vetsci8110278 |
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| author | Molina, Guido Nicolas Amalfi, Sabrina Otero, Ignacio Taboga, Oscar Alberto Molinari, Maria Paula |
| author_browse | Amalfi, Sabrina Molina, Guido Nicolas Molinari, Maria Paula Otero, Ignacio Taboga, Oscar Alberto |
| author_facet | Molina, Guido Nicolas Amalfi, Sabrina Otero, Ignacio Taboga, Oscar Alberto Molinari, Maria Paula |
| author_sort | Molina, Guido Nicolas |
| collection | INTA Digital |
| description | The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16611 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA166112024-02-15T11:08:05Z Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies Molina, Guido Nicolas Amalfi, Sabrina Otero, Ignacio Taboga, Oscar Alberto Molinari, Maria Paula Baculovirus Interferons Swine Antiviral Agents Interferonas Cerdo Autographa californica Viricidas The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved. Instituto de Biotecnología Fil: Molina, Guido Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Molina, Guido Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Amalfi, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Otero, Ignacio. Universidad Maimónides. Centro de Ciencias Veterinarias; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-02-15T11:01:33Z 2024-02-15T11:01:33Z 2021-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16611 https://www.mdpi.com/2306-7381/8/11/278 2306-7381 https://doi.org/10.3390/vetsci8110278 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Veterinary Sciences 8 (11) : 278 (Noviembre 2021) |
| spellingShingle | Baculovirus Interferons Swine Antiviral Agents Interferonas Cerdo Autographa californica Viricidas Molina, Guido Nicolas Amalfi, Sabrina Otero, Ignacio Taboga, Oscar Alberto Molinari, Maria Paula Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title | Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title_full | Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title_fullStr | Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title_full_unstemmed | Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title_short | Baculovirus vectors induce the production of interferons in swine : their potential in the development of antiviral strategies |
| title_sort | baculovirus vectors induce the production of interferons in swine their potential in the development of antiviral strategies |
| topic | Baculovirus Interferons Swine Antiviral Agents Interferonas Cerdo Autographa californica Viricidas |
| url | http://hdl.handle.net/20.500.12123/16611 https://www.mdpi.com/2306-7381/8/11/278 https://doi.org/10.3390/vetsci8110278 |
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