A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice
In this work, we evaluated recombinant receptor binding domain (RBD)-based vaccine formulation prototypes with potential for further clinical development. We assessed different formulations containing RBD plus alum, AddaS03, AddaVax, or the combination of alum and U-Omp19: a novel Brucella spp. prot...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Frontiers Media
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16609 https://www.frontiersin.org/articles/10.3389/fimmu.2022.844837/full https://doi.org/10.3389/fimmu.2022.844837 |
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| author | Coria, Lorena M. Saposnik, Lucas M. Pueblas Castro, Celeste Castro, Eliana Florencia Bruno, Laura A. Stone, William B. Pérez, Paula S. Darriba, Maria Laura Chemes, Lucia B. Alcain, Julieta Mazzitelli, Ignacio Varese, Augusto Salvatori, Melina Auguste, Albert J. Álvarez, Diego E. Pasquevich, Karina A. Cassataro, Juliana |
| author_browse | Alcain, Julieta Auguste, Albert J. Bruno, Laura A. Cassataro, Juliana Castro, Eliana Florencia Chemes, Lucia B. Coria, Lorena M. Darriba, Maria Laura Mazzitelli, Ignacio Pasquevich, Karina A. Pueblas Castro, Celeste Pérez, Paula S. Salvatori, Melina Saposnik, Lucas M. Stone, William B. Varese, Augusto Álvarez, Diego E. |
| author_facet | Coria, Lorena M. Saposnik, Lucas M. Pueblas Castro, Celeste Castro, Eliana Florencia Bruno, Laura A. Stone, William B. Pérez, Paula S. Darriba, Maria Laura Chemes, Lucia B. Alcain, Julieta Mazzitelli, Ignacio Varese, Augusto Salvatori, Melina Auguste, Albert J. Álvarez, Diego E. Pasquevich, Karina A. Cassataro, Juliana |
| author_sort | Coria, Lorena M. |
| collection | INTA Digital |
| description | In this work, we evaluated recombinant receptor binding domain (RBD)-based vaccine formulation prototypes with potential for further clinical development. We assessed different formulations containing RBD plus alum, AddaS03, AddaVax, or the combination of alum and U-Omp19: a novel Brucella spp. protease inhibitor vaccine adjuvant. Results show that the vaccine formulation composed of U-Omp19 and alum as adjuvants has a better performance: it significantly increased mucosal and systemic neutralizing antibodies in comparison to antigen plus alum, AddaVax, or AddaS03. Antibodies induced with the formulation containing U-Omp19 and alum not only increased their neutralization capacity against the ancestral virus but also cross-neutralized alpha, lambda, and gamma variants with similar potency. Furthermore, the addition of U-Omp19 to alum vaccine formulation increased the frequency of RBD-specific geminal center B cells and plasmablasts. Additionally, U-Omp19+alum formulation induced RBD-specific Th1 and CD8+ T-cell responses in spleens and lungs. Finally, this vaccine formulation conferred protection against an intranasal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge of K18-hACE2 mice. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16609 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA166092024-02-15T10:40:58Z A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice Coria, Lorena M. Saposnik, Lucas M. Pueblas Castro, Celeste Castro, Eliana Florencia Bruno, Laura A. Stone, William B. Pérez, Paula S. Darriba, Maria Laura Chemes, Lucia B. Alcain, Julieta Mazzitelli, Ignacio Varese, Augusto Salvatori, Melina Auguste, Albert J. Álvarez, Diego E. Pasquevich, Karina A. Cassataro, Juliana Vaccine Adjuvants Proteinase Inhibitors Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies Mice Coadyuvantes de Vacunas Inhibidores de Proteinasas Coronavirus del Síndrome Respiratorio Agudo Grave 2 Anticuerpos Ratón Germinal Center Cells Células del Centro Germinal In this work, we evaluated recombinant receptor binding domain (RBD)-based vaccine formulation prototypes with potential for further clinical development. We assessed different formulations containing RBD plus alum, AddaS03, AddaVax, or the combination of alum and U-Omp19: a novel Brucella spp. protease inhibitor vaccine adjuvant. Results show that the vaccine formulation composed of U-Omp19 and alum as adjuvants has a better performance: it significantly increased mucosal and systemic neutralizing antibodies in comparison to antigen plus alum, AddaVax, or AddaS03. Antibodies induced with the formulation containing U-Omp19 and alum not only increased their neutralization capacity against the ancestral virus but also cross-neutralized alpha, lambda, and gamma variants with similar potency. Furthermore, the addition of U-Omp19 to alum vaccine formulation increased the frequency of RBD-specific geminal center B cells and plasmablasts. Additionally, U-Omp19+alum formulation induced RBD-specific Th1 and CD8+ T-cell responses in spleens and lungs. Finally, this vaccine formulation conferred protection against an intranasal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge of K18-hACE2 mice. Instituto de Virología Fil: Coria, Lorena M. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Coria, Lorena M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saposnik, Lucas M. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Saposnik, Lucas M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pueblas Castro, Celeste. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Pueblas Castro, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castro, Eliana Florencia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Castro, Eliana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castro, Eliana Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Bruno, Laura A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Bruno, Laura A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stone, William B. Virginia Polytechnic Institute and State University. Fralin Life Science Institute. College of Agriculture and Life Sciences. Department of Entomology; Estados Unidos Fil: Pérez, Paula S. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS); Argentina Fil: Pérez, Paula S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Darriba, Maria Laura. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Darriba, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chemes, Lucia B. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Chemes, Lucia B. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alcain, Julieta. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Alcain, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mazzitelli, Ignacio. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS); Argentina Fil: Mazzitelli, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Varese, Augusto. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS); Argentina Fil: Varese, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Salvatori, Melina. Universidad de Buenos Aires. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS); Argentina Fil: Salvatori, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Auguste, Albert J. Virginia Polytechnic Institute and State University. Fralin Life Science Institute. College of Agriculture and Life Sciences. Department of Entomology; Estados Unidos Fil: Auguste, Albert J. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod-borne Pathogens; Estados Unidos Fil: Álvarez, Diego E. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Álvarez, Diego E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pasquevich, Karina A. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Pasquevich, Karina A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cassataro, Juliana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde; Argentina Fil: Cassataro, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-02-15T10:31:44Z 2024-02-15T10:31:44Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16609 https://www.frontiersin.org/articles/10.3389/fimmu.2022.844837/full 1664-3224 https://doi.org/10.3389/fimmu.2022.844837 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Immunology 13 : 844837 (Febrero 2022) |
| spellingShingle | Vaccine Adjuvants Proteinase Inhibitors Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies Mice Coadyuvantes de Vacunas Inhibidores de Proteinasas Coronavirus del Síndrome Respiratorio Agudo Grave 2 Anticuerpos Ratón Germinal Center Cells Células del Centro Germinal Coria, Lorena M. Saposnik, Lucas M. Pueblas Castro, Celeste Castro, Eliana Florencia Bruno, Laura A. Stone, William B. Pérez, Paula S. Darriba, Maria Laura Chemes, Lucia B. Alcain, Julieta Mazzitelli, Ignacio Varese, Augusto Salvatori, Melina Auguste, Albert J. Álvarez, Diego E. Pasquevich, Karina A. Cassataro, Juliana A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title | A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title_full | A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title_fullStr | A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title_full_unstemmed | A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title_short | A novel bacterial protease inhibitor adjuvant in RBD-based COVID-19 vaccine formulations containing alum increases neutralizing antibodies, specific germinal center B cells and confers protection against SARS-CoV-2 infection in mice |
| title_sort | novel bacterial protease inhibitor adjuvant in rbd based covid 19 vaccine formulations containing alum increases neutralizing antibodies specific germinal center b cells and confers protection against sars cov 2 infection in mice |
| topic | Vaccine Adjuvants Proteinase Inhibitors Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies Mice Coadyuvantes de Vacunas Inhibidores de Proteinasas Coronavirus del Síndrome Respiratorio Agudo Grave 2 Anticuerpos Ratón Germinal Center Cells Células del Centro Germinal |
| url | http://hdl.handle.net/20.500.12123/16609 https://www.frontiersin.org/articles/10.3389/fimmu.2022.844837/full https://doi.org/10.3389/fimmu.2022.844837 |
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